Abstract
Macrophages(M(φs) are not only a kind of immune effector cells but also a kind of antigen-presenting cells(APC). In order to improve their antitumor effect, we transfected interferon-gamma(IFN-γ) gene into Mφs by recombinant adenovirus because IFN-γ is a kind of potent macrophage-activating factor(MAF). High level of IFN-γ could be detected in the supernatants of Mφs after IFN-γ gene transfection and IFN-γ secretion peaked at 20 hour. The cytotoxicity of IFN-γ gene-transfected Mφs increased significantly. The secretion of TNF, IL-1, nitric oxide(NO) also increased to some extent. The results demonstrated that recombinant adenovirus-mediated IFN-γ gene transfection could improve the effector functions of Mφs efficiently.
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This work was supported by the National High Biotechnology Foundation (Z20-01-03).
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Lei, H., Cao, X., Yu, Y. et al. The effector functions of macrophages transfected with interferon-gamma gene mediated by recombinant adenovirus. Chin J Cancer Res 9, 290–294 (1997). https://doi.org/10.1007/BF02974977
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DOI: https://doi.org/10.1007/BF02974977