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Chinese Journal of Cancer Research

, Volume 15, Issue 2, pp 149–151 | Cite as

Up-regulation of cyclooxygenase-2 gene expression correlates with tumor angiogenesis in human breast cancer

  • Dai Zhi-jun 
  • Wang Xi-jing 
  • Liu Xiao-xu 
  • Kang Hua-feng 
  • Jiang Jina-tao 
  • Guan Hai-tao 
  • Zhang Shu-qun 
  • Xue Xing-huan 
  • Xue Feng-jie 
Article
  • 16 Downloads

Abstract

Objective: To study the relationship between cyclooxygenase-2 (COX-2) expression and tumor angiogenesis in human breast cancer. Methods: Archival primary breast carcinomas (n=62), adjacent ductal carcinomain situ (DCIS, n=13) and DCIS alone (n=5) were analyzed for COX-2 and VEGF expression by immunohistochemistry using specific monoclonal antibodies. Microvessel density (MVD) was also examined the using CD34 staining. Results: A significant correlation was found between COX-2 and VEGF expression (P<0.01). Both COX-2 and VEGF were significantly correlated with MVD (P<0.05) andP<0.01, respectively). COX-2 and VEGF genes were overexpressed in tumor specimens as compared with normal epithelia. Conclusion: COX-2 is related to tumor angiogenesis in breast cancer. It is likely that VEGF is one of the most important mediators of the COX-2 angiogenic pathway.

Key words

Breast carcinoma Cyclooxygenase-2 VEGF Microvessel density 

CLC number

R737.9 

References

  1. [1]
    Badawi AF, Archer MC. Effect of hormonal stutus on the expression of the cyclooxygenase 1 and 2 genes and prostaglandin synthesis in rat mammary glands[J]. Prostaglandins Other Lipid Mediat 1998; 56: 167–81.PubMedCrossRefGoogle Scholar
  2. [2]
    Hamid R, Singh J, Rddy BS, et al. Inhibition by dietary menhaden oil of cyclooxygenase-1 and -2 in N-nitrosomethylurea-induced rat mammary tumors[J]. IntJ Oncol 1999; 14:523–8.Google Scholar
  3. [3]
    Nakatsugi S, Ohta T, Kawamori T, et al. Chemoprevention by nimesulide, a selective cyclooxygenase-2 inhibitor, of 2-amino-l-methyl-6-phenylimidazo[4,5-b] pyridine-induced mammary gland carcinogenesis in rats[J]. Jpn J Cancer Res 2000; 91: 886–92.PubMedGoogle Scholar
  4. [4]
    Soslow RA, Dannenberg AJ, Rush D, et al. COX-2 is expressed in human pulmonary, colonic, and mammary tumors[J]. Cancer 2000; 89: 2637–45.PubMedCrossRefGoogle Scholar
  5. [5]
    Weidner n, Semple JP, Wei CH, et al. Tumor angiogenesis and metastasis-correlation in invasive breast carcinoma[J]. N Engl J Med 1991; 324: 1–9.PubMedGoogle Scholar
  6. [6]
    Howe LR, Subbaramaiah K, Brown AMC, et al. Cyclooxygenase-2: a target for the prevention and treatment of breast cancer[J]. Endocr-Related Cancer 2001; 8: 97–114.CrossRefGoogle Scholar
  7. [7]
    Harris RE, Namboodiri KK, Farrar WB. Nonsteroidal anti-inflammatory drugs and breast cancer[J]. Epidemiology 1996; 7: 203–5.PubMedCrossRefGoogle Scholar
  8. [8]
    Harris RE, Kasbari S, Farrar WB. Prospective study of nonsteroidal anti-inflammatory drugs and breast cancer[J]. Oncol Rep 1999; 6: 71–3.PubMedGoogle Scholar
  9. [9]
    Moran EM. Epidemiological and clinical and clinical aspects of nonsteroidal anti-inflammatory drugs and cancer risks[J]. J Environ Pathol Toxicol Oncol 2002; 21: 193–201.PubMedGoogle Scholar
  10. [10]
    Eli Y, Przedecki F, Levin G, et al. Comparative effects of indomethacin on cell cycle progression in tumor cells grown in vbitro and in vivo[J]. Biochem Pharmacol 2001; 61: 565–71.PubMedCrossRefGoogle Scholar
  11. [11]
    Sun WH, Tsuji S, Tsujii M, et al. Cyclooxygenase-2 inhibitor suppress epithelial cell kinetics and delay gastic wound healing in rats[J]. J Gastroenterol Hepatol2000; 15:752–61.PubMedCrossRefGoogle Scholar
  12. [12]
    Costa C, Soares R, Reis-Filho JS, et al. Cyclo-oxygenase 2 expression is associated with angiogenesis and lymph node metastasis in human breast cancer[J]. J Clin Pathol 2002; 55: 429–34.PubMedGoogle Scholar
  13. [14]
    O’Mahony CA, Beauchamp RD, Albo D, et al. cyclooxygenase-2 alters transforming growth factor betal response during intestinal tumor in genesis [J]. Surgery 1999; 26: 364–70.CrossRefGoogle Scholar
  14. [15]
    Kirkpatrick K, Ogunkolade W, Elkak A, et al. The mRNA expression of cyclo-oxygenase-2 (COX-2) and vascular endothelial growth factor (VEGF) in human breast cancer[J]. Curr Med Res Opin 2002; 18: 237–41.PubMedCrossRefGoogle Scholar
  15. [16]
    Gallo O, masini E, Bianchi B, et al. Prognostic significance of cyclooxygenase-2 pathway and angiogenesis in head and neck squamous cell carcinoma [J]. Hum pathol 2002; 33: 708–14.PubMedCrossRefGoogle Scholar
  16. [17]
    Ristimaki A, Sivula A, Lundin J, et al. Prognostic significance of elevated cyclooxygenase-2 expression in breast cancer[J]. Cancer Res 2002; 62: 632–5.PubMedGoogle Scholar

Copyright information

© Chinese Journal of Cancer Research 2003

Authors and Affiliations

  • Dai Zhi-jun 
    • 1
  • Wang Xi-jing 
    • 1
  • Liu Xiao-xu 
    • 1
  • Kang Hua-feng 
    • 1
  • Jiang Jina-tao 
    • 1
  • Guan Hai-tao 
    • 1
  • Zhang Shu-qun 
    • 1
  • Xue Xing-huan 
    • 1
  • Xue Feng-jie 
    • 1
  1. 1.Department of Oncology, Second HospitalX’ian Jiaotong UniversityXi’an

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