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Chinese Journal of Cancer Research

, Volume 15, Issue 2, pp 132–135 | Cite as

Clinical significance of telomerase activity and peripheral venous blood CK-20 expression in bladder transitional cell carcinoma

  • Zhong Wei-de 
  • Zeng Guang-qiao 
  • Cai Yue-bin 
  • Hu Jian-bo 
  • Wei Hong-ai 
Article
  • 8 Downloads

Abstract

Objective: The relationship between peripheral blood CK-20 mRNA expression and tissue telomerase activity in bladder transitional cell carcinoma (TCCB) was investigated to evaluate the feasibility of their combined detection in early-stage diagnosis and prognosis estimation of TCCB. Methods: the blood CK-20 was detected by semi-nested RT-PCR and telomerase activity in tumor tissue was examined with silver-stained TRAP reaction. Results: the blood CK-20 expression and tissue telomerase activity in TCCB were 41% and 93% respectively. No statistical significance was detected among pathological grading and clinical staging (P&s>0.05). Positive correlation was shown between CK-20 expression and telomerase activity with the pathologic grade or clinical stage. Conclusion: combined use of blood CK-20 and tissue telomerase activity detections might be of great importance for clinical diagnosis, treatment and prognosis evaluation.

Keywords

Bladder neoplasm Carcinoma Cytokeratin Telomerase 

CLC number

R737.14 

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References

  1. [1]
    Belldegrun AS, Franklin JR, O’Donnell MA, et al. Superficial bladder cancer: the role of interferon- alpha[J]. J Urol 1998; 159:1793–801.PubMedCrossRefGoogle Scholar
  2. [2]
    Murphy WM. Current status of urinary cytology in the evaluation of bladder neoplasms[J]. Hum Pathol 1990; 21: 886.PubMedCrossRefGoogle Scholar
  3. [3]
    Negrin RS, Blame KG. The use of the polymerase chain reaction for the detection of minimal residual malignant disease[J]. Blood 1991; 78: 255.PubMedGoogle Scholar
  4. [4]
    Kin NW, Piatyszek MA, Prowse KR et al. Specific association of human telomerase activity with immortal Cell and Cancer[J]. Science 1994; 266: 2011–5.CrossRefGoogle Scholar
  5. [5]
    Kamata S, Kageyama Y, Yonese J, et al. Significant toluene reduction in human superficial transitional cell carcinoma[J]. Br J Urol 1996; 78: 707.Google Scholar
  6. [6]
    Ogami M, Ikura Y N, Nishiguchi S et al. Quantitative analysis and in situ localization of human telomerase RNA in chronic liver disease and hepatocellular carcinoma[J]. Lab Invest 1999; 79:15.PubMedGoogle Scholar

Copyright information

© Chinese Journal of Cancer Research 2003

Authors and Affiliations

  • Zhong Wei-de 
    • 1
  • Zeng Guang-qiao 
    • 1
  • Cai Yue-bin 
    • 1
  • Hu Jian-bo 
    • 1
  • Wei Hong-ai 
    • 1
  1. 1.Department of Urology, the First People’s Municipal HospitalGuangzhou Medical CollegeGuangzhou

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