Chinese Journal of Cancer Research

, Volume 15, Issue 2, pp 102–106 | Cite as

Differential expression of genes involved in metabolism between tumorigenitic human leukemia cell lines K562 and K562-n

  • Lü Shu-qing 
  • Xü Xiao-ping 
  • Xia Fang 
  • Jü Xiao-ping 
  • Li Yao 
  • Ying Kang 
  • Mao Yu-min 


Objective: To study the molecular mechanism of different tumorigenicity in nude mice of human leukemia cell lines K562-n and K562. Methods: To analyze the genes differently expressed between K562 and K562-n cells by using cDNA microarray technique. Results: Among the 12800 genes detected, some genes involved in material metabolism and material transport were differently expressed between K562-n and K562 cells. These genes include homo sapiens placenta-specific ATP-binding cassette transporter gene, dihydrodiol dehydrogenase gene, hepatic dihydrodiol dehydrogenase gene, NAD-dependent methylene tetrahydrofolate dehydrogenase cyclohydrolase, lysophosphatidic acid acyltransferase, alpha gene, argininosuccinate lyase gene, mitochondrial isocitrtate dehydrogenase, adhesion protein SQM1 gene, dimethylarginine dimethylamino-hydrolase gene, Ml subunit of ribonucleotide reductase and farnesyl pyrophosphate synthetase gene. Conclusion: The high tumorigenicity of K562-n cells is related to the different expression of some genes concerned with cell metabolism and material transpoert.

Key words

Leukemic cell Tumorigenicity Gene expression Metabolism 

CLC number



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  1. [1]
    Xu XP, Ding XJ, Yan Y, et al. Establishment of leukemia cell line with high tumorigenicity in nude mice and observation of it’s biologic characteristics(in Chinese)[J]. Chin J Hematol 1996; 17: 142–5.Google Scholar
  2. [2]
    Xu XP, Ding XJ, Shi JH, et al. Ectogenesis wild type p53 gene inhibit growth of human leukemic cell in nude mice[J]. Chin J Cancer 1996; 6: 92–5.Google Scholar
  3. [3]
    Lu SHQ, Xu XP, Wang JM, et al. Study of the biological characteristics of human high tumorigenic leukemia cell line K562-n nude mice(in Chinese)[J]. Tumor 2002; 22: 459–62.Google Scholar
  4. [4]
    Schena M, Shaion D, Heller R, et al. Parallel human genome analysis: microarray-based expression monitoring of 1,000 genes. PNAS 1996; 93: 10614.PubMedCrossRefGoogle Scholar
  5. [5]
    Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanatephenol-chloroform extraction [J]. Anal Biochem 1987; 162: 156–9.PubMedCrossRefGoogle Scholar
  6. [6]
    Schena M, shaion D, Davis RW, et al. Quantitative monitoring of gene expression patterns with a complementary DNA microarray[J]. Science 1995; 270:467–70.PubMedCrossRefGoogle Scholar
  7. [7]
    Asada Y, Aoki S, Ishikura S, et al. Roles of His-79 and Tyr-180 of D-xylose/dihydrodiol dehydrogenase in catalytic function[J]. Biochem Biophys Res Commun 2000; 278: 333–7.PubMedCrossRefGoogle Scholar
  8. [8]
    Lou H, Hammond L, Sharma V, et al. Genomic organization and chromosomal localization of a novel human hepatic dihydrodiol dehydrogenase with igh affinity bile acid binding[J]. J Biol Chem 1994; 269: 8416–22.PubMedGoogle Scholar
  9. [9]
    Yang XM, Mackenzie RE. NAD-dependent methylenetrahydrofolate dehydrogenase-methenyltetra-hydrofolate cyclohydrolase is the mammalian homolog of the mitochondrial enzyme encoded by the yeast MIS1 gene[J]. Biochemistry 1993; 32: 11118–23.PubMedCrossRefGoogle Scholar
  10. [10]
    Peri KG, Mackenzie RE. NAD(+)-dependent methyl-lenetetrahydrofolate dehydrogenase-cyclo- hydrolase: detection of the mRNA in normal murine tissues and transcriptional regulation of the gene in cell lines [J]. Biochim Biophys Acta 1993; 1171: 281–7.PubMedGoogle Scholar
  11. [11]
    Allikmets R, Schriml LM, Hutchinson A, et al. A human placenta-specific ATP-binding cassette gene (ABCP) on chromosome 4q22 that is involved in multidrug resistance[J]. Cancer Res 1998; 58:5337–9.PubMedGoogle Scholar
  12. [12]
    West J, Tompkins CK, Balantac N, et al. Cloning and expression of two human lysophosphatidic acid acyltransferase cDNAs that enhance cytoine-induced signaling responses in cells[J]. DNA Cell Biol 1997; 16:691–701.PubMedGoogle Scholar
  13. [13]
    Byrne JA, Little MH, Smith PJ. The Ml subunit of ribonucleotide reductase refines mapping of genetic rearrangements at chromosome llpl5[J]. Cancer Genet Cytogenet 1992; 59: 206–9.PubMedCrossRefGoogle Scholar
  14. [14]
    Tay DL, Bhathal PS, Fox RM, et al. Quantitation of Go and G! phase cells in primary carcinomas. Antibody to Ml subunit of ribonucleotide reductase shows Gl phase restriction point block[J]. J Clin Invest 1991; 87: 519–27.PubMedCrossRefGoogle Scholar
  15. [15]
    Ribas G, Neville M, Wixon JL, et al. Genes encoding three new members of the leukocyte antigen 6 superfamily and a novel member of Ig superfamily, together with genes encoding the regulatory nuclear chloride ion channel protein (hRNCC) and an N omega-N omega-dimethylarginine dimethylamino-hydrolase homologue, are found in a 30-kb segment of the MHC class III regions[J]. J Immunol 1999; 163: 278–87.PubMedGoogle Scholar
  16. [16]
    Mori M, gotoh T. Regulation of nitric oxide production by arginine metabolic enzymes[J]. Biochem Biophys Res Commun 2000; 275: 715–9.PubMedCrossRefGoogle Scholar
  17. [17]
    D’Acquisto F, de Croistofaro F, Maiuri MC, et al. Protective role of nuclear factor kappa B against nitric oxide-induced apoptosis in J774 macrophages[J]. Cell Death Differ 2001; 8: 144–51.PubMedCrossRefGoogle Scholar
  18. [18]
    Jyothi MD, Khar A. Interleukin-2-induced nitric oxide synthase and nuclear factor-kappaB activity in activated natural killer cells and the production of interferon-gamma[J]. Scand J Immunol 2000; 52: 148–55.PubMedCrossRefGoogle Scholar
  19. [19]
    Wilkin DJ, Kutsunai SY, Edwards PA. Isolation and sequence of the human farnesyl pyrophosphate synthetase cDNA. Coordinate regulation of the mRNAs for farnestyl pyrophosphate synthetase, 3-hydroxy-3-methylglutaryl coenzyme A redfuctase, and 3-hydroxy-3-methylglutaryl coenzyme A synthase by phorbol ester[J]. J Biol Chem 1990; 265: 4607–14.PubMedGoogle Scholar
  20. [20]
    Bernal SD, de Villa RS, Wong YC, et al. Congruence of SQM1 protein expression with methotrexate sensitivity and transport[J]. Cancer Invest 1995; 13: 23–30.PubMedCrossRefGoogle Scholar
  21. [21]
    Wong YC, Bernal SD. Reversal of methotrexate resistance in human squamous carcinoma cells by SAM1 liposome[J]. Anticancer Res 1999; 19: 251–4.PubMedGoogle Scholar

Copyright information

© Chinese Journal of Cancer Research 2003

Authors and Affiliations

  • Lü Shu-qing 
    • 1
  • Xü Xiao-ping 
    • 1
  • Xia Fang 
    • 2
  • Jü Xiao-ping 
    • 2
  • Li Yao 
    • 2
  • Ying Kang 
    • 2
  • Mao Yu-min 
    • 2
  1. 1.Department of Hematology, Changhai HospitalSecond Military Medical UniversityShanghai
  2. 2.Institute of GeneticsFudan UniversityShanghai

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