Investigation of the therapeutic effect of expression of trailin vivo on mouse hepatocellular carcinoma
- 16 Downloads
Objective: To construct an eukaryotic expressing plasmid of mouse TRAIL (mTRAIL), and investigate its ability to induce the apoptosis of hepatocellular carcinoma cellsin vitro and in vivo, its inhibitory effect on the growth of hepatocellular carcinoma, and its synergism with pCH510, an eukaryotic expressing plasmid of recombinant human FN polypeptide. Methods: The eukaryotic expressing plasmid of mTRAIL was constructed by RT-PCR and DNA recombination techniques. Gene transfection was performedin vitro and in vivo. The apoptosis rate of hepatocellular carcinoma cells was measured by Flow Cytometry. The apoptosis of hepatocellular carcinoma cells was detected by TdT-mediated dUTP nick end labeling (TUNEL) and histochemistry techniques. The inhibitory effect of gene transfection on solid tumor was observed in mice. Results: The cDNA of mTRAIL was amplified by RT-PCR from the RNA of mouse spleen cells, and cloned into the eukaryotic expressing vector pcDNA3.1. The recombinant plasmid was designated as pXl. The BHK cells transfected with plasmid pXl could attack H22 hepatocellular carcinoma cells and induce the apoptosis of them. The transfection of plasmid pX1 through injection into mouse muscles could inhibit the growth of hepatocellular carcinoma by inducing the apoptosis of tumor cells. Plasmid pX1 and pCH510 had a synergistic inhibitory effect on the hepatocellular carcinoma growth. Conclusion: Plamid pX1 could be expressed in cells andin vivo in mouse. The expression of pX1in vivo andin vitro could induce the apoptosis of hepatocellular carcinoma cells and inhibit the growth of hepatocellular carcinoma. Plasmid pX1 and pCH510 had a synergistic inhibitory effect on the hepatocellular carcinoma growth.
Key wordsTRAIL Eukaryotic Expression Tumor therapy
Unable to display preview. Download preview PDF.
- Ye Shiqiao, Feng Zuohua, Li Dong, et al. Construction and expression of eukaryotic expressing vector pCH510 of polypeptide CH50 and its chemotaxis and antitumor function by transfectionin vivo(in Chinese)[J]. Chin J Cancer Biother 2001; 8: 23–6.Google Scholar
- Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning: A Laboratory Manual[M]. 2nd ed. New York: Cold Spring Harbor Laboratory Press, 1989. P???Google Scholar
- Sun Hong, Zhang Xiyin, Feng Youji. Investigation on the therapeutic effect of recombinant interleukin 2 in combination with chemotherapy on relapse and metastasis of oophoroma(in Chinese)[J]. Chin J Cancer Biother 1999; 6: 312–3.Google Scholar
- Huang Bo, Feng Zuohua, Zhang Guimei, et al. The therapeutic effect of eukaryotic expressing vector pCH510 of recombinant polypeptide of FN linking chemotherapy on tumor-bearing mice(in Chinese)[J]. Chin J Cancer Biother, 2001, 8: 168–172.Google Scholar