Inducement of antitumor-immunity by DC activated by Hsp70-H22 tumor antigen peptide
- 23 Downloads
Objective: To investigate the feasibility of decreasing the dosage of tumor antigen peptides by dendritic cell (DC)-presenting and the characteristics of modification of DC by heat shock protein (Hsp70) and antigen peptides. Methods: Peptides were bound to Hsp70 and used to modify DCin vitro. The metabolism of the modified DC and the cytokines secreted by the modified DC were determined. The activation of lymphocytes by the modified DC and Hsp70-H22 peptides was tested. The cytotoxicity of the activated lymphocytes to H22 tumor cells was analyzed. The inhibitory effect of tumor in mice by the injection of DC and Hsp70-H22 peptides was tested. Results: 0.15 µ g of H22 peptides bound with Hsp70 could make 2 ×105 DC mature. 4×103 matured DC could activate 2×106 lymphocytes. The same amount of lymphocytes could be activated to produce similar cytotoxicity to tumor cells by either DC modified by 0.003µg of peptides bound with Hsp70 or by direct stimulation with 0.15 µg of peptides bound with Hsp70. The dosage of peptides could be reduced by about 50 folds if the modified DC was used for injection instead of Hsp70-peptides. Peptides from normal hepatocytes, bound with Hsp70, could not make DC mature, nor activate lymphocytes through DC. Conclusion: The dosage of Hsp70-H22 peptides can be reduced significantly by DC-presenting to activate lymphocytes. Peptides from normal cells could not activate lymphocytes by either Hsp70-presenting or DC-presenting and they have little chance to induce autoimmunity.
Key wordsHsp70 H22 peptide Dosage DC-presenting Anti-tumor immunity
Unable to display preview. Download preview PDF.
- Huang Bo, Feng Zuohua, Zhang Guimei, et al. Investigation on the effect of peptides mixture from tumor cells inducing anti-tumor specific immune response[J]. Science in China (Series C) 2002; 32:165–71.Google Scholar
- Huang Bo, Feng Zuo-hua, Zhang Gui-mei, et al. The therapeutic effect of eukaryotic expressing vector pCH510 of recombinant polypeptide of FN linking chemotherapy on tumor-bearing mice(in Chinese)[J]. Chin J Cancer Biother 2001; 8:168–72.Google Scholar
- Huang Bo, Feng Zuo-hua, Zhang Gui-mei, et al. Experimental study on the therapy of murine tumor by mixed antigen peptides from H22 hepatocarcinoma cells presented by rhHsp70(in Chinese)[J]. Chin J Microbiol Immunol 2002; 22:160–3.Google Scholar