Abstract
MIT method was applied to assay the cytotoxicity of three reversors, verapamil (VER), dipyridamole (DPM) and cyclosporin (CSA) in KS62, K562/ADM and KB cell lines. S-P immunocytocbemical technique was applied to detect expressions of oncoproteins or tumor suppressor oncoproteins in these tumor cell lines before or after treatment with these reversors. Results showed that three reversors were capable of inhibiting to a certain extent growth of K562 or KB cell lines and reversing greatly adriamycin (ADM)-resistance in K562/ADM cell line. DPM and CsA were observed to inhibit, partly or wholly, expressions of p53, pl6, bcl-2, p21 or cerbB-2 oncoproteins. VER showed whole inhibition of expressions of p53, pl6, p21 and bcl-2 and partly expression of p53 oncoprotein in K562 cell line. These results suggest that growth inhibition in K562 or KB cell lines by the reversors may be associated with partial or whole inhibition of expressions of p53, p16, p21 or cerbB-2 oncoproteins. Inhibitions of expressions p53, pl6, p21 oncoproteins by VER but not DPM or CsA, may be respossible for reversing activity of VER for ADM-resistance in K562/ADM cell line.
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Xie, Z., Lin, X., Zhou, D. et al. Effect of drug-resistance reversors on expressions of oncogenes or tumor suppressor oncogenes of human tumor cell lines. Chin J Cancer Res 9, 111–115 (1997). https://doi.org/10.1007/BF02974675
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DOI: https://doi.org/10.1007/BF02974675