Abstract
The role of sex hormones in hepatic lipid peroxidation, and in hepatic aldehyde oxidase and xanthine oxidase activities were investigated using rat liver homogenates. It was observed that male rat had a significantly greater content of malondialdehyde in liver than female. Among the sex hormones tested, estradiol, one of female hormones, markeldy inhibited the formation of lipid peroxides in liver tissuesin vitro. Especially, the inhibitory effect of estradiol appeared more remarkably in Fe2-induced lipid peroxidation. The hepatic xanthine oxidase activity was decreased about 15% by 106M estradiol, whereas, the aldehyde oxidase activity was almost completely disappeared at the same concentration of estradiol. It implies that sex differences in lipid peroxidation is attributed to the suppression of free radical generating system by estradiol.
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References Cited
Beedham, C., Molybdenum hydrolase as drug metabolizing enzymes.Drug Met. Rev., 16, 119–156 (1985).
Bindoli, A., Cavallini, L., Rigobello, M. P., and Lisa, F. D., Modification of the xanthine-converting enzyme of perfused rat heart during ischemia and oxidative stress.Free Radical Biology and medicine, 4, 163–167 (1988).
Branzoli, U., and Massey, V., Preparation of aldehydeoxidase in its native and deflavo forms.J. Biol. Chem., 249, 4339–4345 (1974).
Comporti, M., Lipid peroxidation and cellular damage in toxic liver injuries.Lab. Invest. 53, 599–623 (1985).
Corte, E. D., and Stripe, F. The regulation of rat liver xanthine oxiase.J. Biol. Chem., 244, 3855–3863 (1969).
Dominique, B. T., Guerin, M. C., and Torreilles, J., Is malonaldehyde a valuableindicator of lipid peroxidation?.Biochem. Pharmacol., 44, 985–988 (1992).
Granger, D. N., Höllwarth, M. E., and Parks, D. A., Role of oxygen-drived free radicals.Acta Physiol Scand. Suppl., 548, 47–63 (1986).
Hashimi, R. S., Kachole, M. S., and Pawar, S. S., Alteration in hepatic microsomal mixed-function oxidase system during different levels of food restriction in adult male and female rats.J. Nutr., 116, 682–688 (1986).
Hearse, D. J., Manning, A. S., Downey, J. M., and Yellon, D. M., Xanthine oxidase: a critical mediator of myocardial injury during ischemia and reperfusion?Acta Physiol. Scand. Suppl., 548, 65–78 (1986).
Hirafuji, M., and Ogura, J., Lipid peroxidation modifies the effect of phenolic anti-inflammatory drug on prostaglandin biosynthesis.Biochem. Pharmacol., 34, 933 (1985).
Holman, W. I. M., and Wiegand, C., The chemical conversion of nicotinic acid and nicotinamide to derivatives of N-Methyl-2-Pyridone by methylation and oxidation.Biochem. J., 43, 423–426 (1948).
Li, J. L., Okada, S., Hamazaki, S., Deng, I. L., and Midorikawa, O., Sex differences in ferric nitrotriacetate-induced lipid peroxidation and nephrotoxicity in mice.Biochim. Biophys. Acta, 963, 82–87 (1988).
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., Protein measurement with the Folin phenol reagent.J. Biol. Chem., 193, 265–272 (1951).
Lunec, J., Free radicals: Their involvement in disease process.Ann. Clin. Biochem., 27, 173–182 (1990).
Mackelvey, T. C., Hollwarth, M. E., Granger, D. N., Urikelander, T. D. and Jones, H., Mechanisms of conversion of xanthine dehydrogenase to xanthine oxidase in ischemic rat liver and kidney.Am. J. Physiol., 254, G753–760 (1988).
McCord, J. M., The superoxide free radical: It's biochemistry and pathophysiology.Surgery, 94, 412 (1983).
Mezey, E., Sharma, S. Rennie, L., and Potter, J. J., Sex differences in gastric alcohol dehydrogenase activity in Sprague-Dawley rats.Gastroenterol., 103, 1804–1810 (1992).
Mizuta, Y., Mitzuta, K. and Kohno, M., Quantitative analysis of active oxygen generated in Fe-peroxide reaction system using ESR spin trapping mrthod.Med. Biochem. Chem. Asp. Free Radicals, 817–820 (1989).
Mottley, C., Robinson, R. E., and Mason, R. P., Free radical formation in the oxidation of malonaldehyde and acetylacetone by peroxidase enzymes.Arch. Biochim. Biophys., 289, 153–160 (1991).
Ohkawa, H., Ohishi, N., and Yagi, K., Assay for lipid peroxides in animal tissue by thiobarbituric acid reaction.Anal. Biochem., 95, 351 (1979).
Pikul, J., Leszcynski, F., and Kummerow, F. A. Evaluation of three modified TBA methods for measuring lipid peroxidation.J. Agric. Food Chem., 37, 1309–1313 (1989).
Ross, D., A radical perspective.J. Pharm. Pharmacol., 41, 505–511 (1989).
Stanulovic, M., and Chaykin, S., Metabolic origins of the pyridones of N′-methylnicotinamide in man and rat.Arch. Biochim. Biophys. 145, 35–42 (1971).
Stripe, F., and Corte, E. D., The regulation of rat liver xanthine oxidase.J. Biol. Chem., 244, 3855–3863 (1969).
Yaki, K., Lipid peroxides and human diseases.Chem. Phys. Lipid, 45, 337–343 (1987).
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Huh, K., Shin, US., Choi, JW. et al. Effect of sex hormones on lipid peroxidation in rat liver. Arch. Pharm. Res. 17, 109–114 (1994). https://doi.org/10.1007/BF02974233
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DOI: https://doi.org/10.1007/BF02974233