Abstract
The cytotoxicity of animal venoms (snakes, insects and marine animals) was measured against SNU-1 (stomach cancer cells) by dye uptake assay (MTT method). And also L-amino acid oxidase (AAO) activity of the venoms was compared. Among them, the venom fromOphiophagus hannah (king cobra) showed a strong AAO activity as well as a high potent cytotoxicity. Cytotoxic protein having a AAO was then partially purified by HPLC-GPC and two fractions (Fr. I and Fr. II) were collected. The IC50 values of Fr. I and Fr. II were 0.19 μg/ml and 1.36 μg/ml, respectively. The results suggested that the cytotoxicity of king cobra venom may be due to its AAO activity.
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Ahn, M.Y., Lee, B.M. & Kim, Y.S. Cytotoxicity and L-amino acid oxidase activity of animal venoms. Arch. Pharm. Res. 20, 13–16 (1997). https://doi.org/10.1007/BF02974035
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DOI: https://doi.org/10.1007/BF02974035