Abstract
A stereospecific HPLC method has been developed for the resolution of the enantiomers of salbutamol in human urine. After solid-phase extraction and derivatization with 2,3,4,6-tetra-O-acetyl-β-D-glucopyranosyl isothiocyanate, the diastereomeric derivatives were resolved (Rs=1. 83) on 5 μm octadecylsilan column using 35% acetonitrile in 0.05M ammonium acetate buffer (pH=6) as a mobile phase with electrochemical detection. The diastereomeric derivatives were formed within 30 min. The detection limit of each enantiomer was 20 ng/ml (S/N=3).
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Kim, K.H., Kim, T.K. Resolution of salbutamol enantiomers in human urine by reversed-phase high performance liquid chromatography after derivatization with 2,3,4,6-tetra-O-acetyl-β-D-glycopyranosyl isothiocyanate. Arch. Pharm. Res. 21, 217–222 (1998). https://doi.org/10.1007/BF02974031
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DOI: https://doi.org/10.1007/BF02974031