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Bioconversion of aniline to acetaminophen and overproduction of acetaminophen byStreptomyces spp.

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Abstract

In order to obtain acetaminophen, a popular analgesic-antipyretic, though microbial p-hydroxylation and N-acetylation of aniline, variousStreptomyces strains were screened. Aniline N-acetylation activity was rather ubiquitous but p-hydroxylation activity was selective. Microbial conversion pathway of aniline to acetaminophen was considered to be through N-acetylation and p-hydroxylation orvice versa. However, depending on species used, o-hydroxylation and its degradation activity (S. fradiae) and acetaminophen degradation activity (S. coelicolar) were also detected. Among the screenedStreptomyces strains.S. fradiae NRRL 2702 showed the highest acetanilide p-hydroxylation activity (2–3% conversion rate). Furthermore, inS. fradiae carbon source and its concentration, phosphate ion concentration and pH of growth medium were found to play the crucial roles in p-hydroxylation activity. Through the proper combination of factors mentioned above, the ten times more activity (26–30% conversion rate) was attained.

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  1. College of Pharmacy, Seoul National University, 151-742, Seoul, Korea

    Hyung Jong Jin, Ae Kyung Park & Sang Sup Lee

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  1. Hyung Jong Jin
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  2. Ae Kyung Park
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  3. Sang Sup Lee
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Jin, H.J., Park, A.K. & Lee, S.S. Bioconversion of aniline to acetaminophen and overproduction of acetaminophen byStreptomyces spp.. Arch. Pharm. Res. 15, 41–47 (1992). https://doi.org/10.1007/BF02973982

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  • DOI: https://doi.org/10.1007/BF02973982

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