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Apport de la cytométrie en flux à la technique du lavage bronchiolo-alvéolaire

Flow cytometry as an adjunct to bronchiolo-alveolar lavage

  • Published:
Acta Endoscopica

Résumé

Le lavage broncho-alvéolaire (LBA) est une méthode largement utilisée pour identifier l’alvéolite dans diverses affections respiratoires et principalement dans les maladies interstitielles pulmonaires. La cytométrie en flux permet le phénotypage immunologique des sous-groupes de lymphocytes et de monocytes et l’identification de microorganismes ainsi que l’évaluation quantitative du contenu en ADN des cellules tumorales. Cette technique demande à être validée mais constitue un complément utile de l’analyse des liquides de LBA et présente un intérêt particulier en immunopathologie pulmonaire et dans le suivi des manifestations respiratoires après greffe de poumon.

Summary

Bronchoalveolar lavage (BAL) is a widely used method to asess alveolitis in various respiratory disorders and mainly in the interstitial lung diseases. Flow cytometry allowed immunophenotyping of lymphocytes and monocytes subsets, identification of micro-organisms and quantification of tumoral cells DNA content. This technique needs to be validate but may represent a useful complement in the BAL fluids analysis and have specific interrest in lung immunopathology and assessment of the respiratory events following a lung transplantation.

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Références

  1. CAPRON F. — Le lavage broncho-alvéolaire.Ann. Pathol., 1990, 10,4, 232–246.

    CAS  PubMed  Google Scholar 

  2. DEINLEIN E., SANDER U., GREINER C., HORNSTEIN O. — Diagnostic significance of flow cytometric DNA analysis applied for the detection of cancer cells in bronchial washing fluid.Analyt. Quant. Cytol. Histol., 1988,10, 5, 360–364.

    CAS  Google Scholar 

  3. ELMENDORF S., McSHARRY J., LAFFIN J., FOGLEMAN D., LEHMAN J. — Detection of an early cytomegalovirus antigen with two-color quantitative flow cytometry.Cytrometry, 1988,9, 254–260.

    Article  CAS  Google Scholar 

  4. FUHR J., KATTINE A., SULLIVAN T., NELSON S. — Flow cytometric analysis of pulmonary fluids and cells for the detection of maligancies.Am. J. Pathol., 1992,141, 1, 211–215.

    CAS  PubMed  PubMed Central  Google Scholar 

  5. GRATZIOU C., CARROL M., WALLS A., HOWARTH P., HOLGATE S.T. — Early changes in T lymphocytes recovered by bronchoalveolar lavage after local allergen challenge of asthmatic airways.Am. Rev. Respir. Dis., 1992,145, 1259–1264.

    Article  CAS  PubMed  Google Scholar 

  6. GUILLON J.M., AUTRAN B., DENIS M., FOURET P., PLATA F., MAYAUD C., AKOUN G. — Human immunodeficiency virus-related lymphocytic alveolitis.Chest., 1988,94, 1264–1270.

    Article  CAS  PubMed  Google Scholar 

  7. LAPINSKY S., GLENCROSS D., CAR N., KALLENBACH J., ZWI S. — Quantification and assessment of viability of pneumocystis carinii organisms by flow cytometry.J. Clin. Microbiol., 1991, 29,5, 911–915.

    CAS  PubMed  PubMed Central  Google Scholar 

  8. PADOVAN C., BEHR J., ALLMELING A.M., GERLACH J., VOGELMEIER C., KROMBACH F. — Immunophenotyping of lymphocyte subsets in bronchoalveolar lavage fluid.J. Immunol. Methods., 1992,147, 27–32.

    Article  CAS  PubMed  Google Scholar 

  9. PIROZYNSKI M. — Bronchoalveolar lavage in the diagostic of peripheral, primary lung cancer.Chest., 1992,102, 372–374.

    Article  CAS  PubMed  Google Scholar 

  10. RABINOWICH H., ZEEVI A., YOUSSEM S., PARADIS I., DAUBER J., KORMOS R., HARDESTY R., GRIFFIT B., DUQUESNOY R. — Alloreactivity of lung biopsy and bronchoalveolar lavage-derived lymphocytes from pulmonary transplant patients: Correlation with acute rejection and bronchiolitis obliterans.Clin. transplantation, 1990,4, 376–384.

    Google Scholar 

  11. REYNOLDS H. — Bronchoalveolar lavage.Am. Rev. Respir. Dis., 1987,135, 250–263.

    CAS  PubMed  Google Scholar 

  12. SHENNIB H., NGUYEN D. — Bronchoalveolar lavage in lung transplantation.Ann. Thorac. Surg., 1991,51, 335–340.

    Article  CAS  PubMed  Google Scholar 

  13. USUI Y., AIDA H., KIMULA Y., MIURA H., AOYAGI Y., NAKAYAMA M., TAKAYAMA S. — A case of Cyclophosphamide-induced interstitial pneumonitis diagnosed by bronchoalveolar lavage.Respiration, 1992,59, 125–128.

    Article  CAS  PubMed  Google Scholar 

  14. WALKER C., BODE E., BOER L., HANSEL T., BLASER K., VIRCHOW J. — Allergic and non allergic asthmatics have distinct patterns of T-cell activation and cytokine production in peripheral blood and bronchoalveolar lavage.Am. rev. respir. dis., 1992,146, 109–115.

    Article  CAS  PubMed  Google Scholar 

  15. WHITE D., MATTHAY R. — Noninfectious pulmonary complications of infection with the human immunodeficiency virus.Am. rev. respir. dis., 1989,140, 1763–1787.

    Article  CAS  PubMed  Google Scholar 

  16. WILSON J., DJUKANOVIC R., HOWARTH P., HOLGATE S.T. — Lymphocyte activation in bronchoalveolar lavage and peripheral blood in atopic asthma.Am. Rev. Respir. Dis., 1992,145, 958–960.

    Article  CAS  PubMed  Google Scholar 

  17. YOSS E., BERD D., COHN J., PETERS S. — Flow cytometric evaluation of bronchoscopic washings and lavage fluid for DNA aneuploidy as an adjunct in the diagnosis of lung cancer and tumors metastatic to the lung.Chest., 1989,96, 1, 54–59.

    Article  CAS  PubMed  Google Scholar 

  18. ZEEVI A., RABINOVITCH H., PARADIS I. — Lymphocyte activation in bronchoalveolar lavages from heart lung transplant recipients.Transplant. Proc., 1988,20, 189–192.

    CAS  PubMed  Google Scholar 

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Author notes

  1. Valérie Costes

    Present address: Service d’Anatomie Pathologique B, Hôpital Lapeyronie, 34000, Montpellier, France

Authors and Affiliations

  1. Service des Maladies Respiratoires, Hôpital Arnaud de Villeneuve, Montpellier

    P. Chanez

  2. Service commun de cytométrie en flux, INSERM U291, Montpellier, France

    Ch. Duperray

  3. Service d’Anatomie Pathologique B, Hôpital Lapeyronie, 34000, Montpellier, France

    P. Baldet

Authors
  1. Valérie Costes
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  2. P. Chanez
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  3. Ch. Duperray
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  4. P. Baldet
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Costes, V., Chanez, P., Duperray, C. et al. Apport de la cytométrie en flux à la technique du lavage bronchiolo-alvéolaire. Acta Endosc 23, 295–299 (1993). https://doi.org/10.1007/BF02970003

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  • DOI: https://doi.org/10.1007/BF02970003

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