Abstract
The microarray of DNA probes with 5′-NH2 and 5′-Tex/3′-NH2 modified terminus on 10 μm carboxylate functional beads surface in the presence of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) is characterized in the present paper. It was found that the microarray capacity of DNA probes on the beads surface depends on the pH of the aqueous solution, the concentration of DNA probe and the total surface area of the beads. On optimal conditions, the minimum distance of 20 mer single-stranded DNA probe microarrayed on beads surface is about 14 nm, while that of 20 mer double-stranded DNA probes is about 27 nm. If the probe length increases from 20 mer to 35 mer, its microarray density decreases correspondingly. Mechanism study shows that the binding mode of DNA probes on the beads surface is nearly parallel to the beads surface.
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References
Hughes, M. P., Morgan, H., Dielectrophoretic characterization and separation of antibody-coated submicrometer latex spheres, Anal. Chem., 1999, 71: 3441.
Schena, M., Shalon, D., Davis, R. W. et al., Quantitative monitoring of gene expression patterns with a complementary DNA microarray, Science, 1995, 270: 467.
Guo, Z., Guilfoyle, R. A., Thiel, A. J. et al., Direct fluorescence analysis of genetic polymorphisms by hybridization with oligonucleotide arrays on glass supports, Nucleic Acids Res., 1994, 22: 5456.
Schena, M., Shalon, D., Heller, R. et al., Parallel human genome analysis: microarray-based expression monitoring of 1 000 genes, Proc. Natl. Acad. Sci. USA, 1996, 93: 10614.
Livshits, M. A., Mizabekov, A. D., Theoretical analysis of the kinetics of DNA hybridization with gel-immobilized oligonucleotides, Biophys. J., 1996, 71: 2795.
Hall, M., Kazakova, I., Yao, Y. M., High sensitivity immunoassays using particulate fluorescent labels, Anal. Biochem., 1999, 272: 165.
Xu, X. H., Bard, A. J., Immobilization and hybridization of DNA on an aluminum(III) alkanebisphosphonate thin film with electrogenerated chemiluminescent detection, J. Am. Chem. Soc., 1995, 117: 2627.
Lam, K. S., Lebl, M., Krchnack, V., One beads one compound, Chem. Rev., 1997, 97: 411.
Ohlmeyer, M. H. J., Swanson, R. N., Dillard, L. W. et al., Complex synthetic chemical libraries indexed with molecular tags, Proc. Natl. Acd. Sci. USA, 1993, 90: 10922.
Hall, M., Kazakov, I., Yao, Y. M., High sensitivity immunoassays using particulate fluorescence labels, Anal. Biochem., 1999, 272: 165.
Okano, K., Takahashi, S., Yasuda, K. et al. Using microparticle labeling and counting for attomole-level detection in heterogeneous immunoassay, Anal. Biochem., 1992, 202: 120.
Martens, C., Bakker, A., Rodriguez, A. et al., A generic particle-based nonradioactive homogeneous multiplex method for high-throughput screening using microvolume fluorimetry, Anal. Biochem., 1999, 273: 20.
Buranda, T., Jones, G. M., Nolan, J. P. et al., Ligand receptor dynamics at streptavidin-coated particle surfaces: A flow cy-tometric and spectrofluorimetric study, J. Phys. Chem. B, 1999, 103: 3399.
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Huang, C., Li, Y., Huang, X. et al. Microarray of DNA probes on carboxylate functional beads surface. Sc. China Ser. B-Chem. 43, 435–442 (2000). https://doi.org/10.1007/BF02969450
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DOI: https://doi.org/10.1007/BF02969450