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Protection of peroxynitrite-induced DNA damage by dietary antioxidants

Archives of Pharmacal Research volume 29, Article number: 213 (2006) Cite this article

Abstract

The present study was undertaken to test the hypothesis that dietary antioxidants protect DNA damage induced by peroxynitrite, a potent physiological inorganic toxin. The present study showed that dietary antioxidants such as (−)-epigallocatechin, gallate, quercerin, rutin, resveratrol, and ursolic acid inhibit single strand breaks in supercoiled plasmid DNA induced by 3-morpholinosyndromine N-ethylcarbamide (SIN-1), a generator of peroxynitrite, through the reaction between nitric oxide and superoxide anion. The formation of 8-hydroxy-2′-deoxyguanosine (8-OH-dG) in calf thymus DNA by SIN-1 was also inhibited by dietary antioxidants. When U937 cells were incubated with 1 mM SIN-1 bolus, a significant increase of 8-OH-dG level was observed. However, oxidative DNA damage was significantly lower in the cells pre-treated with dietary antioxidants when cells were exposed to SIN-1.

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References

  • Afanas'ev, I. B., Dorozhko, A. I., Brodskii, A. V., Kostyuk, V. A., and Potapovitch, A. I., Chelating and free radical scavenging mechanisms of inhibitory action of rutin and quercetin in lipid peroxidation.Biochem. Pharmacol., 38, 1763–1769 (1989).

    PubMed  Article  Google Scholar 

  • Ames, B. N., Gold, L. S., and Willet, W. C., The causes and prevention of cancer.Proc. Natl. Acad. Sci. USA, 92, 5258–5265 (1995).

    PubMed  Article  CAS  Google Scholar 

  • Balanehru, S. and Nagarajan, B., Protective effect of oleanolic acid and ursolic acid against lipid peroxidation.Biochem. Int., 24, 981–990 (1991).

    PubMed  CAS  Google Scholar 

  • Beckman, J. S., Beckman, T. W., Chen, J., Marshall, P. A., and Freeman, B. A., Apparent hydroxyl radical production by peroxynitrite: implications for endothelial injury from nitric oxide and superoxide.Proc. Natl. Acad. Sci. USA, 87, 1620–1624 (1990).

    PubMed  Article  CAS  Google Scholar 

  • Beckman, J. S., Carson, M., Smith, C. D., and Koppenol, W. H., ALS, SOD and Peroxynitrite.Nature, 364, 584 (1993).

    PubMed  Article  CAS  Google Scholar 

  • Carreras, M. C., Pargament, G. A., Catz, S. D., Poderosa, J. J., and Boveris, A., Kinetics of nitric oxide and hydrogen peroxide production and formation of peroxynitrite during the respiratory burst of human neutrophils.FEBS Lett., 341, 65–68 (1994).

    PubMed  Article  CAS  Google Scholar 

  • Galati, G. and O'Brien, P. J., Potential toxicity of flavonoids and other dietary phenolics: Significance for their chemopreventive and anticancer properties.Free Radic. Biol. Med., 37, 287–303 (2004).

    PubMed  Article  CAS  Google Scholar 

  • Hertog, M. G., Hollman, P. C., Katan, M. B., and Kromhout, D., Intake of potentially anticarcinogenic flavonoids and their determinants in adults in The Netherlands.Nutr. Cancer. 20, 21–29 (1993).

    PubMed  CAS  Article  Google Scholar 

  • Ischiropoulos, H., Zhu, L., and Beckman, J. S., Peroxynitrite formation from macrophage-derived nitric oxide.Arch. Biochem. Biophys., 298, 446–451 (1992).

    PubMed  Article  CAS  Google Scholar 

  • Juedes, M. J. and Wogan, G. N., Peroxynitrite-induced mutation spectra of pSP189 following replication in bacteria and in human cells.Mut. Res., 349, 51–61 (1996).

    Google Scholar 

  • Kaur, H. and Halliwell, B., Evidence for nitric oxide-mediated oxidative damage in chronic inflammation. Nitrotyrosine in serum and synovial fluid from rheumatoid patients.FEBS Lett., 350, 9–12 (1994).

    PubMed  Article  CAS  Google Scholar 

  • Kooy, N. W. and Royall, J. A., Agonist-induced peroxynitrite production from endothelial cells.Arch. Biochem. Biophys., 310, 352–359 (1994).

    PubMed  Article  CAS  Google Scholar 

  • Koppenol, W. H., Moreno, J. J., Pryor W. A., Ischiropoulos, H., and Beckman, J. S., Peroxynitrite, a cloaked oxidant formed by nitric oxide and superoxide.Chem. Res. Toxicol., 5, 834–842 (1992).

    PubMed  Article  CAS  Google Scholar 

  • Park, J.-W. and Floyd, R. A., Lipid peroxidation products mediate the formation of 8-hydroxydeoxyguanosine in DNA.Free Radic. Biol. Med., 12, 245–250 (1992).

    PubMed  Article  CAS  Google Scholar 

  • Park, J.-W. and Floyd R. A., Generation of strand breaks and formation of 8-hydroxy-2′-deoxyguanosine in DNA by a thiol/Fe3+/O2-catalyzed oxidation system.Arch. Biochem. Biophys., 12, 245–250 (1992).

    CAS  Google Scholar 

  • Radi, R., Beckman, J. S., Bush, K. M., and Freeman, B. A., Peroxynitrite oxidation of sulfhydryls. The cytotoxic potential of superoxide and nitric oxide.J. Biol. Chem., 266, 4244–4250 (1991).

    PubMed  CAS  Google Scholar 

  • Salgo, M. G. and Pryor, W. A., Trolox inhibits peroxynitrite-mediated oxidative stress and apoptosis in rat thymocytes.Arch. Biochem. Biophys., 333, 482–488 (1996).

    PubMed  Article  CAS  Google Scholar 

  • Shibutani, S., Takeshita, M., and Grollman, A. P., Insertion of specific base during DNA synthesis past the oxidation-damaged base 8-oxodG.Nature, 349, 431–434 (1991).

    PubMed  Article  CAS  Google Scholar 

  • Struthers, L., Patel, R., Clark, J., and Thomas, S., Direct detection of 8-oxodeoxyguanosine and 8-oxoguanine by avidin and its analogues.Anal. Biochem., 255, 20–31 (1998).

    PubMed  Article  CAS  Google Scholar 

  • Sun, A. Y., Simonyi, A., and Sun, G. Y., The “French paradox” and beyond: Neuroprotective effects of polyphenols.Free Radic. Biol. Med., 32, 314–318 (2002).

    PubMed  Article  CAS  Google Scholar 

  • Sundaresan, M., Yu, Z. Y., Ferrans, C. J., Irani, K., and Finkel, T., Requirement for generation of H2O2 for platelet-derived growth factor signal transduction.Science, 270, 296–299 (1995).

    PubMed  Article  CAS  Google Scholar 

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Authors and Affiliations

  1. School of Life Sciences and Biotechnology and Agro-Biotechnology Education Center, Kyungpook National University, 702-701, Taegu, Korea

    Hye Kyung Moon, Eun Sun Yang & Jeen Woo Park

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  1. Hye Kyung Moon
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  2. Eun Sun Yang
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  3. Jeen Woo Park
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Correspondence to Jeen Woo Park.

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Moon, H.K., Yang, E.S. & Park, J.W. Protection of peroxynitrite-induced DNA damage by dietary antioxidants. Arch Pharm Res 29, 213 (2006). https://doi.org/10.1007/BF02969396

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  • DOI: https://doi.org/10.1007/BF02969396

Key words

  • Dietary antioxidants
  • Peroxynitrite
  • DNA damage