Abstract
As part of our research on phytochemicals that exert protective effects against diseases related to reactive nitrogen specie, we have evaluated the scavenging activity of the yellow leaves ofGinkgo biloba on ONOO−. The methanol extract and ethyl acetate fraction obtained from yellow leaves ofG. biloba evidenced a marked scavenging activity on authentic, ONOO−. Repeated column chromatography of the active ethyl acetate soluble fraction on silica gel, Sephadex LH-20, and RP-18, resulted in the purification of 15 known compounds, including sciadopitysin (1), ginkgolide B (2), bilobalide (3), isoginkgetin (4), kaempferol (5), luteolin (6), protocatechuic acid (7), bilobetin (8), amentoflavone (9), β-sitosterol glucopyranoside (10), kaempferol 3-O-rhamnopyranoside (11), kaempferol 3-O-glucopyranoside (12), kaempferol 3-O-[6‴-O-p-coumaroyl-β-D-glucopyranosyl(1→2)-α-L-rhamnopyranoside] (13), kaempferol 3-O-rutinoside (14), and 6-hydroxykynurenic acid (15). Among the compounds isolated, flavonoids (5, 6 and11–14), protocatechuic acid (7), and 6-hydroxykynurenic acid (15) all exhibited marked scavenging activities on authentic ONOO−. The IC50 values of5–7, 11–14 and 15 were as follows: 2.86±0.70, 2.30±0.04, 2.85±0.10, 5.60±0.47, 4.16±1.65, 2.47±0.15, 3.02±0.48, and 6.24±0.27 μM respectively. DL-Penicillamine (IC50=4.98±0.27 μM) was utilized as a positive control. However, the other compounds (1–4, 8–10) exerted no effects against ONOO−.
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Hyun, S.K., Jung, H.A., Chung, H.Y. et al. In vitro peroxynitrite scavenging activity of 6-hydroxykynurenic acid and other flavonoids fromGingko biloba yellow leaves. Arch Pharm Res 29, 1074–1079 (2006). https://doi.org/10.1007/BF02969294
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DOI: https://doi.org/10.1007/BF02969294