Archives of Pharmacal Research

, Volume 29, Issue 7, pp 566–569 | Cite as

Influence of ginsenoside Rb1 on brain neurosteroid during acute immobilization stress

  • Sang Hee Lee
  • Byung Hwa Jung
  • Sang Yoon Choi
  • Sun Yeou Kim
  • Eunjoo H Lee
  • Bong Chul Chung
Articles Drug Devopment

Abstract

This study examined whether or not acute stress is linked to increases in the neurosteroid levels, which is a well-known neurotransmitters associated with stress stimuli. The ginsenoside, Rb1, was tested in order to better understand its potential effects on altering the neurosteroid levels and ultimately attenuating stress. The optimal stressed condition was checked by measuring the 5a-dihydroprogesterone (DHP) and allopregnanolone (THP) levels in the brain after immobilization stress at various times. Based on this result, an acute stress model was set up to give 30 min of immobilization stress. The DHP and THP brain levels of the stressed mice were then investigated after administering Rb1 orally (10 mg/kg). These results were compared with the neurosteroid level in the stressed mice not given Rb1. Saline was administered orally to the nonstressed mice to check the placebo effect. Acute immobilization stress induced an increase in the THP and DHP concentration in the frontal cortex and cerebellum. When Rb1 was administered orally prior to immobilization stress, the THP level in the frontal cortex and cerebellum was significantly lower than that in the stressed animals not given Rb1. On the other hand, the DHP level was lower in the cerebellum only. This suggests that the metabolism of the brain neurosteroids in linked to psychological stress, and Rb1 attenuates the stress-induced increase in neurosteroids.

Key words

Immobilization stress Neurosteroid Frontal cortex Cerebellum Ginsenoside Rb1 GC-MS 

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Copyright information

© The Pharmaceutical Society of Korea 2006

Authors and Affiliations

  • Sang Hee Lee
    • 1
    • 2
  • Byung Hwa Jung
    • 1
  • Sang Yoon Choi
    • 3
  • Sun Yeou Kim
    • 2
  • Eunjoo H Lee
    • 2
  • Bong Chul Chung
    • 1
  1. 1.Bioanalysis & Biotransformation Research CenterKorea Institute of Science & TechnologySeoulKorea
  2. 2.Graduate School of East-West Medical ScienceKyung Hee UniversitySeoulKorea
  3. 3.Korea Food Research InstituteSongnamKorea

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