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Utilisation de la réaction de polymérase en chaîne (PCR) et de l’hybridationin situ pour la détection d’infections du tube digestif

The use of the polymerase chain reaction andIn-situ hybridization in Detecting infections of the gastrointestinal tract

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Acta Endoscopica

Résumé

La réaction de polymérisation en chaîne (PCR) et l’hybridationin situ ont conduit à une explosion d’informations dans presque tous les domaines de la recherche scientifique. Au cours de ces dernières années, ces techniques ont commencé à quitter le royaume exclusif de la recherche fondamentale vers la routine quotidienne et le laboratoire d’analyses cliniques. Des tests sensibles et spécifiques qui permettent de détecter et de typer toutes sortes de germes allant des mycobactéries aux microsporidies font lentement leur chemin dans l’exercice clinique. Ce qui suit est une revue des techniques de PCR et d’hybridationin situ qui peuvent être utilisées pour le diagnostic de maladies infectieuses du tube digestif. Bien que ces tests soient largement utilisés à des fins de recherche ou épidémiologiques, je pense que ce n’est qu’une question de temps avant qu’ils ne deviennent des analyses cliniques de routine.

Summary

The polymerase chain reaction and in situ hybridization have led to an information explosion in nearly all fields of scientific research. In recent years, these techniques have started to move from the exclusive realm of the basic scientist to the daily routine of the clinical laboratory. Sensitive and specific assays to detect and speciate everything from mycobacteria to microsporidia are slowly making their way into clinical practice. The following is a review of polymerase chain reaction and in-situ hybridization assays which can be used to diagnose infectious diseases of the gastrointestinal tract. Although many of these tests are largely used for research and epidemiological purposes, I believe it is only a matter of time before they become routine clinical laboratory tests.

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Références

  1. PERSING D.H. Polymerase Chain Reaction: Trenches to Benches.J. Clin. Microbiol., 1991,29, 1281–1285.

    PubMed  CAS  Google Scholar 

  2. RETTINGER S.D., HAFENRICHTER D.G., FLYE M.W. Clinical Diagnostic Applications of the Polymerase Chain Reaction.Arch. Surg. 1993,128, 1253–1259.

    PubMed  CAS  Google Scholar 

  3. NUOVO G.J., GORGONE G.A., MACCONNELL P., MARGIOTTA M., GOREVIC P.D. —In situ localization of PCR-amplified human and viral cDNAs.PCR Methods Appl, 1992,2, 117–123.

    PubMed  CAS  Google Scholar 

  4. BASHIR M.S., LEWIS F.A., QUIRKE P., LEE A., DIXON M.F. —In situ hybridisation for the identification of Helicobacter pylori in paraffin wax embedded tissue.J. Clin. Pathol., 1994,47, 862–864.

    Article  PubMed  CAS  Google Scholar 

  5. KARTTUNEN T.J., GENTA R.M., YOFFE B., HACHEM C.Y., GRAHAM D.Y., EL-ZATTARI F.A.K. — Detection of Helicobacter pylori in Paraffin-Embedded Gastric Biopsy Specimens byIn situ Hybridization.Am. J. Clin. Pathol., 1996,106, 305–311.

    PubMed  CAS  Google Scholar 

  6. EL-ZAATARI F.A.K., NGUYEN A.-M.H., GENTA R.M., KLEIN P.D., GRAHAM D.Y. — Determination of Helicobacter pylori Status by Reverse Transcription-Polymerase Chain Reaction. Comparison with Urea Breath Test.Digestive Diseases and Sciences, 1995,40, 109–113.

    Article  PubMed  CAS  Google Scholar 

  7. MAPSTONE N.P., LYNCH D.A.F., LEWIS F.A., AXON A.T.R., TOMPKINS D.S., DIXON M.F., QUIRKE P. — Identification of Helicobacter pylori DNA in the mouths and stomachs of patients with gastritis using PCR.J. Clin. Pathol., 1993,46, 540–543.

    Article  PubMed  CAS  Google Scholar 

  8. KAWAMATA O., YOSHIDA H., HIROTA K., YOSHIDA A., KAWAGUCHI R., SHIRATORI Y., OMATA M. — Nested-Polymerase Chain Reaction for the Detection of Helicobacter pylori Infection with Novel Primers Designed by Sequence Analysis or Urease A Gene in Clinically Isolated Bacterial Strains.Biochemical and Biophysical Research Communications, 1996,219, 266–272.

    Article  PubMed  CAS  Google Scholar 

  9. MATSUKURA N., ONDA M., TOKUNAGA A., KATO S., YAMASHITA K., OHBAYASHI M. — Detection of Helicobacter pylori DNA in gastric juice by the polymerase chain reaction: Comparison with findings in bacterial culture and the detection of tissue IgA and serum IgG antibodies against Helicobacter pylori.J. Gastroenterol., 1995,30, 689–695.

    Article  PubMed  CAS  Google Scholar 

  10. FABRE R., SOBHANI I., LAURENT-PUIG P., HEDEF N., YAZIGI N., VISSUZAINE C., RODDE I., POTET F., MIGNON M., ETIENNE J.P., BRAQUET M. — Polymerase chain reaction assay for the detection of Helicobacter pylori in gastric biopsy specimens: comparison with culture, rapid urease test, and histopathological tests.Gut, 1994,35, 905–908.

    Article  PubMed  CAS  Google Scholar 

  11. CHONG S.K.F., LOU Q., ASNICAR M.A., ZIMMERMAN S.E., CROFFIE J.M., LEE C.-H., FITZGERALD J.F. — Helicobacter pylori Infection in Recurrent Abdominal Pain in Childhood: Comparison of Diagnostic Tests and Therapy.Pediatrics, 1995,96, 211–215.

    PubMed  CAS  Google Scholar 

  12. LAGE A.P., GODFROID E., FAUCONNIER A., BURETTE A., BUTZLER J.-P., BOLLEN A., GLUPCZYNSKI Y. — Diagnosis of Helicobacter pylori Infection by PCR: Comparison with Other Invasive Techniques and Detection of cagA Gene in Gastric Biopsy Specimens.J. Clin. Microbiol., 1995,33, 2752–2756.

    PubMed  CAS  Google Scholar 

  13. RELMAN D.A., SCHMIDT T.M., MACDERMOTT R.P., FALKOW S. — Identification of the Uncultured Bacillus of Whipple’s Disease.N. Engl. J. Med., 1992,327, 293–301.

    PubMed  CAS  Google Scholar 

  14. MAIWALD M., MEIER-WILLERSEN H.J., HARTMANN M., VON HERBAY A. — Detection of Tropheryma whippelii DNA in a Patient with AIDS.J. Clin. Microbiol., 1995,33, 1354–1356.

    PubMed  CAS  Google Scholar 

  15. VON HERBAY A., DITTON H.-J., MAIWALD M. — Diagnostic Application of a Polymerase Chain Reaction Assay for the Whipple’s Disease Bacterium to Intestinal Biopsies.Gastroenterology, 1996,110, 1735–1743.

    Article  Google Scholar 

  16. LOWSKY R., ARCHER G.L., FYLES G., MINDEN M., CURTIS J., MESSNER H., ATKINS H., PATTERSON B., WILLEY B.M., MCGEER A. — Brief Report: Diagnosis of Whipple’s Disease by Molecular Analysis of Peripheral Blood.N. Engl. J. Med., 1994,331, 1343–1346.

    Article  PubMed  CAS  Google Scholar 

  17. BRIAN M.J., FROSOLONO M., MURRAY B.E., MIRANDA A., LOPEZ E.L., GOMEZ H.F., CLEARY T.G. — Polymerase Chain Reaction for Diagnosis of Enterohemorrhagic Escherichia coli Infection and Hemolytic-Uremic Syndrome.J. Clin. Microbiol., 1992,30, 1801–1806.

    PubMed  CAS  Google Scholar 

  18. BEGUM D., STROCKBINE N.A., SOWERS E.G., JACKSON M.P. — Evaluation of a Technique for Identification of Shiga-Like Toxin-Producing Escherichia coli by Using Polymerase Chain Reaction and Digoxigenin-Labeled Probes.J. Clin. Microbiol., 1993,31, 3153–3156.

    PubMed  CAS  Google Scholar 

  19. GANNON V.P., KING R.K., KIM J.Y., THOMAS E.J. — Rapid and sensitive method for detection of Shiga-like toxin-producing Escherichia coli in ground beef using the polymerase chain reaction.Appl. Environ. Microbiol., 1992,58, 3809–3815.

    PubMed  CAS  Google Scholar 

  20. RAMOTAR K., WALDHART B., CHURCH D., SZUMSKI R., LOUIE T.J. — Direct Detection of Verotoxin-Producing Escherichia coli in Stool Samples by PCR.J. Clin. Microbiol., 1995,33, 519–524.

    PubMed  CAS  Google Scholar 

  21. FRANKEL G., RILEY L., GIRON J.A., VALMASSOI J., FRIEDMANN A., STROCKBINE N., FALKOW S., SCHOOLNIK G.K. — Detection of Shigella in Feces Using DNA Amplification.The Journal of Infectious Diseases, 1990,161, 1252–1256.

    PubMed  CAS  Google Scholar 

  22. KUHL S.J., TANG Y.J., NAVARRO L., GUMERLOCK P.H., SILVA J.J.R. — Diagnosis and monitoring of Clostridium difficile infections with the Polymerase Chain Reaction.Clin. Infect. Dis., 1993,16, s234–8.

    PubMed  Google Scholar 

  23. IBRAHIM A., LIESACK W., STACKEBRANDT E. — Polymerase Chain Reaction-gene probe detection system specific for pathogenic strains of Yersinia enterocolitica.J. Clin. Microbiol., 1992,30, 1942–1947.

    PubMed  CAS  Google Scholar 

  24. GHOSSEIN R.A., ROSS D.G., SALOMON R.N., RABSON A.R. Rapid detection and species identification of mycobacteria in paraffin-embedded tissues by polymerase chain reaction.Diagn. Mol. Pathol., 1992,1, 185–191.

    Article  PubMed  CAS  Google Scholar 

  25. COOK S.M., BARTOS R.E., PIERSON C.L., FRANK T.S. — Detection and characterization of atypical mycobacteria by the polymerase chain reaction.Diagn. Mol. Pathol., 1994,3, 53–58.

    Article  PubMed  CAS  Google Scholar 

  26. JACKSON K.M., EDWARDS R.M., BOWDEN D.S., LESLIE D.E. — Evaluation of the Roche Amplicor Polymerase Chain Reaction System for Detection of Mycobacterium tuberculosis complex in Specimens.Pathology, 1996,28, 65–67.

    Article  PubMed  CAS  Google Scholar 

  27. STRICKLER J.G., MANIVEL J.C., COPENHAVER C.M., KUBIC V.L. — Comparison ofIn situ Hybridization and Immunohistochemistry for Detection of Cytomegalovirus and Herpes Simplex Virus.Hum. Pathol. 1990,21, 443–448.

    Article  PubMed  CAS  Google Scholar 

  28. WU G.-D., SHINTAKU I.P., CHIEN K., GELLER S.A. — A Comparison of Routine Light Microscopy, Immunohistochemistry, andIn situ Hybridization for the Detection of Cytomegalovirus in Gastrointestinal Biopsies.The American Journal of Gastroenterology, 1989,84, 1517–1520.

    PubMed  CAS  Google Scholar 

  29. KEH W.C., GERBER M.A. —In situ Hybridization for Cytomegalovirus DNA in AIDS Patients.Am. J. Pathol., 1988,131, 490–496.

    PubMed  CAS  Google Scholar 

  30. ROBEY S.S., GAGE W.R., KUHAJDA F.P. — Comparison of Immunoperoxidase and DNAIn situ Hybridization Techniques in the Diagnosis of Cytomegalovirus Colitis.Am. J. Clin. Pathol., 1988,89, 666–671.

    PubMed  CAS  Google Scholar 

  31. LINDEN M.D., TUBBS R.R., BAUER T.W., PROFFITT M.R., RUTHERFORD I. — Detection of Cytomegalovirus Infections: Comparison ofIn situ Hybridization with Immunohistology.Am. J. Clin. Pathol., 1988,90, 493.

    Google Scholar 

  32. RATNAMOHAN V.M., MATHYS J.M., MCKENZIE A., CUNNINGHAM AL. — HMCV-DNA is detected more frequently than infectious virus in blood leukocytes of immunocompromised patients: a direct comparison of culture-immunofluorescence and PCR for detection of HCMV in clinical specimens.J. Med. Virol., 1992,38, 252–259.

    Article  PubMed  CAS  Google Scholar 

  33. BITSCH A., KIRCHNER H., DENNIN R., HOYER J., FRICKE L., STEINHOFF J., SACK K., BEIN G. — The long persistence of CMV DNA in the blood of renal transplant patients after recovery from CMV infection.Transplantation, 1993,56, 108–113.

    Article  PubMed  CAS  Google Scholar 

  34. GERNA G., ZIPETO D., PAREA M., REVELLO M.G., SILINI E., PERCIVALLE E., ZAVATTONI M., GROSSI P., MILANESI G. — Monitoring of human cytomegalovirus infections and ganciclovir treatment in heart transplant recipients by determination of viremia, antigenemia, and DNAe-mia.J. Infect. Dis. 1991,164, 488–498.

    PubMed  CAS  Google Scholar 

  35. BRAINARD J.A., GREENSON J.K., VESY C.J., TESI R.J., PAPP A.C., SNYDER P.J., WESTERN L., PRIOR T.W. — Detection of Cytomegalovirus in Liver Transplant Biopsies.Transplantation, 1994,57, 1753–1757.

    PubMed  CAS  Google Scholar 

  36. MICHEL D., MARRE E., HAMPL W., ROCZKOS J., MULLER S., HERTENSTEIN B., KERN P., HEYMER B., SALZBERGER B., ARASTEH K., MERTENS T. — Intestinal Cytomegalovirus Disease in Immunocompromised Patients May Be Ruled Out by Search for Cytomegalovirus DNA in Stool Samples.J. Clin. Microbiol., 1995,33, 3064–3067.

    PubMed  CAS  Google Scholar 

  37. TOMITA T., CHIGA M., LENAHAN M., BALACHANDRAN N. — Identification of herpes simplex virus infection by immunoperoxidase andIn situ hybridization methods.Virchows. Archiv. A. Pathol. Anat., 1991,419, 99–105.

    Article  CAS  Google Scholar 

  38. VESY C.J., GREENSON J.K., PAPP A.C., SNYDER P.J., QUALMAN S.J., PRIOR T.W. — Evaluation of Celiac Disease Biopsies for Adenovirus 12 DNA Using a Multiplex Polymerase Chain Reaction.Modern Pathology, 1993,6, 61–64.

    PubMed  CAS  Google Scholar 

  39. WILDE J., YOLKEN R., WILLOUGHBY R., EIDEN J. — Improved detection of rotavirus shedding by polymerase chain reaction.The Lancet, 1991,337, 323–326.

    Article  CAS  Google Scholar 

  40. XU L., HARBOUR D., MCCRAE M.A. — The application of polymerase chain reaction to the detection of rotaviruses in faeces.Journal of Virological Methods, 1990,27, 29–38.

    Article  PubMed  CAS  Google Scholar 

  41. JIANG X., WANG J., GRAHAM D.Y., ESTES M.K. — Detection of Norwalk Virus in Stool by Polymerase Chain Reaction.J. Clin. Microbiol., 1992,30, 2529–2534.

    PubMed  CAS  Google Scholar 

  42. BALATBAT A.B., JORDAN G.W., TANG Y.J., SILVA J. — Detection of Cryptosporidium parvum DNA in Human Feces by Nested PCR.J. Clin. Microbiol., 1996,34, 1769–1772.

    PubMed  CAS  Google Scholar 

  43. LAXER M.A., D’NICUOLA M.E., PATEL R.J. — Detection of Cryptosporidium Parvum DNA in Fixed, Paraffin-Embedded Tissue by the Polymerase Chain Reaction.Am. J. Trop. Med. Hyg., 1992,47, 450–455.

    PubMed  CAS  Google Scholar 

  44. MAYER C.L., PALMER C.J. — Evaluation of PCR, Nested PCR, and Fluorescent Antibodies for Detection of Giardia and Cryptosporidium Species in Wastewater.Appl. Environ. Microbiol., 1996,62, 2081–2085.

    PubMed  CAS  Google Scholar 

  45. GREENSON J.K., BELITSOS P.C., YARDLEY J.H., BARTLETT J.G. — AIDS Enteropathy: Occult Enteric Infections and Duodenal Mucosal Alterations in Chronic Diarrhea.Annals of Internal Medicine, 1991,114, 366–372.

    PubMed  CAS  Google Scholar 

  46. WEBER R., BRYAN R.T., SCHWARTZ D.A., OWEN R.L. — Human Microsporidial Infections.Clin. Microbiol. Rev., 1994,7, 426–461.

    PubMed  CAS  Google Scholar 

  47. DAVID F., SCHUITEMA A.R.J., SARFATI C., LIGUORY O., HARTSKEERL R.A., DEROUIN F., MOLINA J.-M. — Detection and Species Identification of Intestinal Microsporidia by Polymerase Chain Reaction in Duodenal Biopsies from Human Immunodeficiency Virus-Infected Patients.JID, 1996,174, 874–877.

    PubMed  CAS  Google Scholar 

  48. WEBER R., SAUER B., SPYCHER M.A., DEPLAZES P., KELLER R., AMMANN R., BRINER J., LUTHY R. — Detection of Septata intestinalis in Stool Specimens and Coprodiagnostic Monitoring of Successful Treatment with Albendazole.Clinical Infectious Diseases, 1994,19, 342–345.

    PubMed  CAS  Google Scholar 

  49. FRANZEN C., MULLER A., HEGENER P., HARTMANN P., SALZBERGER B., FRANZEN B., DIEHL V., FATKENHEUER G. — Polymerase chain reaction for microsporidian DNA in gastrointestinal biopsy specimens of HIV-infected patients.AIDS, 1996,10, F23-F27.

    Article  PubMed  CAS  Google Scholar 

  50. KATZWINKEL-WLADARSCH S., LIEB M., HEISE W., LOSCHER T., RINDER H. — Direct amplification and species determination of microsporidian DNA from stool specimens.Tropical Medicine and International Health, 1996,1, 373–378.

    Article  PubMed  CAS  Google Scholar 

  51. FEDORKO D.P., NELSON N.A., CARTWRIGHT C.P. — Identification of Microsporidia in Stool Specimens by Using PCR and Restriction Endonucleases.J. Clin. Microbiol., 1995,33, 1739–1741.

    PubMed  CAS  Google Scholar 

  52. MAHBUBANI M.H., BEJ A.K., PERLIN M., SCHAEFER F.W., JAKUBOWSKI W., ATLAS R.M. — Detection of Giardia Cysts by Using the Polymerase Chain Reaction and Distinguishing Live from Dead Cysts.Appl. Environ. Microbiol., 1991,57, 3456–3461.

    PubMed  CAS  Google Scholar 

  53. ACUNA-SOTO R., SAMUELSON J., DE GIROLAMI P., ZARATE L., MILLAN-VELASCO F., SCHOOLNICK G., WIRTH D. — Application of the Polymerase Chain Reaction to the Epidemiology of Pathogenic and Nonpathogenic Entamoeba Histolytica.Am. J. Trop. Med. Hyg., 1993,48, 58–70.

    PubMed  CAS  Google Scholar 

  54. TACHIBANA H., KOBAYASHI S., OKUZAWA E., MASUDA G. — Detection of Pathogenic Entamoeba Histolytica DNA in Liver Abscess Fluid by Polymerase Chain Reaction.International Journal for Parasitology, 1992,22, 1193–1196.

    Article  PubMed  CAS  Google Scholar 

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  1. J. K. Greenson M.D.

    Present address: Department of Pathology, University of Michigan Hospitals, Room 2G332, 1500 E. Medical Center Drive, Box 0054, 48109-0054, Ann Arbor, Michigan, USA

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    Greenson, J.K. Utilisation de la réaction de polymérase en chaîne (PCR) et de l’hybridationin situ pour la détection d’infections du tube digestif. Acta Endosc 27, 145–153 (1997). https://doi.org/10.1007/BF02968934

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