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La technique de PCR est une méthode plus précise pour déterminer la monoclonalité des lymphomes gastriques

The polymerase chain reaction is a more accurate method to determine monoclonality in gastric lymphomas

  • Published:
Acta Endoscopica

Résumé

Bien que les critères morphologiques en soient bien établis, le diagnostic du lymphome gastrique à partir de biopsies gastriques endoscopiques reste très difficile. Pour confirmer la nature néoplasique de l’infiltrat, il faut prouver qu’il est monoclonal. Puisque les succès obtenus en immuno-histochimie demeurent limités, de nouvelles techniques ont été développées au niveau moléculaire. Sa simplicité, son coût réduit, sa rapidité et sa grande sensibilité expliquent le succès de la technique de PCR. Sa grande sensibilité est cependant un inconvénient car un infiltrat monoclonal peut être retrouvé non seulement dans un lymphome gastrique mais aussi lors d’une gastrite. Ainsi, les résultats de cette technique doivent toujours être corrélés avec la morphologie des biopsies gastriques et le tableau clinique du patient.

Summary

Although the morphological criteria are well-established, the diagnosis of gastric lymphoma on gastric endoscopic biopsies remains very difficult. To confirm the neoplastic origin of the infiltrate, the monoclonal nature of the infiltrate has to be proven. Since the success of immunohistochemistry is limited, new techniques on molecular level have been developed. Simplicity, cost-effectiveness, rapidity and high sensitivity explain the success of the polymerase chain reaction. Its high sensitivity is however a drawback of the technique as a monoclonal infiltrate is not only found in gastric lymphomas but also in gastritis. Hence the results of this technique have always to be correlated with the morphology of the gastric biopsies and the clinical presentation of the patient.

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Références

  1. GREINER A., MÜLLER-HERMELINK H.K. — Recent advances in gastric extranodal B-cell lymphoma.Current Diagnostic Pathology, 1996,3, 91–98.

    Article  Google Scholar 

  2. DE KOSTER E., BUSET M., NYST J.F., DELTENRE M. — Gastric screening prospects.Eur. J. Cancer Prevention, 1993,2, 263–268.

    Article  CAS  Google Scholar 

  3. WEISENBURGER D. D. — Epidemiology of non-Hodgkin’s lymphoma: Recent findings regarding an emerging epidemic.Ann.Oncol., 1994,5 (Suppl 1), S19-S24.

    Google Scholar 

  4. CHAN J.K.C., ISAACSON P.G. — Relationship between high-grade lymphoma and low-grade B-cell mucosa-associated lymphoid tissue lymphoma (MAL Toma) of the stomach.Am. J. Pathol., 1990,136, 1153–1164.

    PubMed  CAS  Google Scholar 

  5. ARISTA-NASR J., JIMENEZ A., KEIRNS C., LARRAZA O., LARRIVA-SAHD J. — The role of the endoscopic biopsy in the diagnosis of gastric lymphoma: A morphologic and immunohistochemical reappraisal.Hum. Pathol., 1991,22, 339–348.

    Article  PubMed  CAS  Google Scholar 

  6. HARRIS N.L., JAFF E.S., STEIN H.et al. — A Revised European-American Classification of Lymphoid Neoplasms: A Proposal From The International Lymphoma Study Group.Blood, 1994,84, 1361–1392.

    PubMed  CAS  Google Scholar 

  7. KNOWLES D. M. — Lymphoid cell markers. Their distribution and usefulness in the immunophenotypic analysis of lymphoid neoplasms.Am. J. Surg. Pathol., 1985,9, Supplement:85–108.

    Google Scholar 

  8. INAGAKI H., NONAKA M., NAGAYA S., TATEYAMA H., SASAKI M., EIMOTO T. — Monoclonality in gastric lymphoma detected in formalin-fixed, paraffin-embedded endoscopic biopsy specimens using immunohistochemistry, in situ hybridization, and polymerase chain reaction.Diagn. Mol. Pathol., 1995,4, 32–38.

    Article  PubMed  CAS  Google Scholar 

  9. WOLF B. C., MARTIN A. W., REE H. J.et al. — Non-Hodgkin’s lymphomas of the gastrointestinal tract. An evaluation of paraffin section immunostaining.Am. J. Clin. Pathol., 1990,93, 233–239.

    PubMed  CAS  Google Scholar 

  10. DISS T. C., PAN L., PENG H., WOTHERSPOON A. C., ISAACSON P. G. — Sources of DNA for detecting B cell monoclonality using PCR.J. Clin. Pathol., 1994,47, 493–496.

    Article  PubMed  CAS  Google Scholar 

  11. SOUTHERN E. M. — Detection of specific sequences among DNA fragments separated by gel electrophoresis.J. Mol. Biol., 1975,98, 503–517.

    Article  PubMed  CAS  Google Scholar 

  12. GRODY W. W., GATTI R. A., NAEIM F. — Diagnostic molecular pathology.Mod. Pathol., 1989,2, 553–568.

    PubMed  CAS  Google Scholar 

  13. ARNOLD A., COSSMAN J., BAKHSHI A., JAFFE E. S., WALDMANN T. A., KORSMEYER S. J. — Immunoglobulin-gene rearrangements as unique clonal markers in human lymphoid neoplasms.N. Engl. J. Med., 1983,309, 1593–1599.

    Article  PubMed  CAS  Google Scholar 

  14. WAN J. H., TRAINOR K. J., BRISCO M. J., MORLEY A. A. — Monoclonality in B-cell lymphoma detected in paraffin wax embedded sections using the polymerase chain reaction.J. Clin. Pathol., 1990,43, 888–890.

    Article  PubMed  CAS  Google Scholar 

  15. DEANE M., NORTON J. D. — Detection of immunoglobulin gene rearrangement in B cell neoplasias by polymerase chain reaction gene amplification.Leukemia and Lymphoma, 1991,5, 9–22.

    Article  Google Scholar 

  16. OSBORNE B.M., PUGH W.C. — Practicality of molecular studies to evaluate small lymphocytic proliferations in endoscopic gastric biopsies.Am. J. Surg. Pathol., 1992,16, 838–844.

    Article  PubMed  CAS  Google Scholar 

  17. MULLIS K., FALOONA F., SCHARF S., SAIKI R., HORN G., EHRLICH H. — Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. Cold Spring Harb. Symp.Quant. Biol., 1986,51, 263–273.

    CAS  Google Scholar 

  18. SIOUTOS N., BAGG A., MICHAUD G. Y.,et al. — Polymerase chain reaction versus Southern blot hybridization.Diagn. Mol. Pathol. 1995,4, 8–13.

    Article  PubMed  CAS  Google Scholar 

  19. MIHALOV M. L., HUBER S., RACHWALSKI E., PRICE J. S., HOSSO J. M., DIZIKES G. J. — Detection of clonal immunoglobulin heavy chain gene rearrangements in cases of suspected lymphoproliferative disorders: Comparison of polymerase chain reaction and southern blot analysis.South Med. J., 1996,89, 39–45.

    PubMed  CAS  Google Scholar 

  20. SKLAR J. — Antigen receptor genes: Structure, function, and techniques for analysis of their rearrangements, in Knowles DM (ed): Neoplastic Haematopathology, Baltimore, MD,Williams & Wilkins, 1992, pp 215–244.

    Google Scholar 

  21. KNOWLES D. M. — Immunophenotypic and immunogenotypic approaches useful in distinguishing benign and malignant lymphoid proliferations.Seminars in Oncology, 1993,20, 6:583–610.

    PubMed  CAS  Google Scholar 

  22. KORSMEYER S. J., HIETER P. A., RAVETCH J. V., POPLACK D. G., WALDMANN T. A., LEDER P. — Developmental hierarchy of immunoglobulin gene rearrangements in human leukemic pre-B-cells.Proc. Natl. Acad. Sci. USA, 1981,78, 7096–7100.

    Article  PubMed  CAS  Google Scholar 

  23. BERMAN J., MELLIS S. J., POLLOCK R.et al. — Content and organization of the human Ig VH locus: definition of three new VH families and linkage to the Ig CH locus.EMBO, 1988,7, 727–738.

    CAS  Google Scholar 

  24. ICHIHARA Y., MATSUOKA H., KUROSAWA Y. — Organization of human immunoglobulin heavy chain diversity loci.EMBO, 1988,7, 4141–4150.

    CAS  Google Scholar 

  25. TONEGAWA S. — Somatic generation of antibody diversity.Nature, 1983,302, 575–581.

    Article  PubMed  CAS  Google Scholar 

  26. CLEARY M. L., CHAO J., WARNKE R., SKLAR J. — Immunoglobulin gene rearrangement as a diagnostic criterion of B-cell lymphoma.Proc. Natl. Acad. Sci. USA, 1984,81, 593–597.

    Article  PubMed  CAS  Google Scholar 

  27. CLAVEL C., BINNINGER I., POLETTE M., BOUTTERIN M.C., BIREMBAUT P.H. — Polymérase chain reaction (P.C.R.) et pathologie.Ann. Pathol., 1993,13, 88–96.

    PubMed  CAS  Google Scholar 

  28. SCHORDERET D. F. — La polymerase chain reaction: principes de base. Schweiz. Rundsch.Med. Prax., 1994,83, 588–594.

    CAS  Google Scholar 

  29. SAIKI R. K., GELFAND D. H., STOFFEL S.,et al. — Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.Science, 1988,239, 487–491.

    Article  PubMed  CAS  Google Scholar 

  30. NARAYANAN S. — Overview of principles and current uses of DNA probes in clinical and laboratory medicine.Ann. Clin. Lab. Sci., 1992,22, 353–376.

    PubMed  CAS  Google Scholar 

  31. BILLIALD P., GOYFFON M. — PCR — Principe et perspectives en biologique clinique.Ann. Pharmaceutiques Françaises, 1995,53, 155–162.

    CAS  Google Scholar 

  32. CHIEN A., EDGAR D. B., TRELA J. M. — Deoxyribonucleic acid polymerase from the extreme thermophile Thermus aquaticus.J. Bacteriol., 1976,127, 1550–1557.

    PubMed  CAS  Google Scholar 

  33. LANDEGREN U., KAISER R., CASKEY C. T., HOOD L. — DNA diagnostics — Molecular techniques and automation.Science, 1988,242, 229–237.

    Article  PubMed  CAS  Google Scholar 

  34. MC CARTHY K. P., SLOANE J. P., WIEDEMANN L. M. — Rapid method for distinguishing clonal from polyclonal B cell populations in surgical biopsy specimens.J. Clin. Pathol., 1990,43, 429–432.

    Article  CAS  Google Scholar 

  35. ACHILLE A., SCARPA A., MONTRESOR M.,et al. — Routine application of polymerase chain reaction in the diagnosis of monoclonality of B-cell lymphoid proliferations.Diagn. Mol. Pathol., 1995,4, 14–24.

    Article  PubMed  CAS  Google Scholar 

  36. BRISCO M. J., TAN L. W., ORSBORN A. M., MORLEY A. A. — Development of a highly sensitive assay, based on the polymerase chain reaction, for rare B-lymphocyte clones in a polyclonal population.Br. J. Haematol., 1990,75, 163–167.

    Article  PubMed  CAS  Google Scholar 

  37. RAMASAY I., BRISCO M., MORLEY A. — Improved PCR method for detecting monoclonal immunoglobulin heavy chain rearrangement in B cell neoplasms.J. Clin. Pathol., 1992,45, 770–775.

    Article  Google Scholar 

  38. INGHIRAMI G., SZABOLICS M. J., YEEN H. T., CORRADINI P., CESARMAN E., KNOWLES D. M. — Detection of immunoglobulin gene rearrangement of B cell Non Hodgkin’s lymphomas and leukemias in fresh, unfixed and formalin-fixed, paraffin-embedded tissue by polymerase chain reaction.Lab. Invest., 1993,68, 746–757.

    PubMed  CAS  Google Scholar 

  39. DEANE M., NORTON J. D. — Immunoglobulin gene “fingerprinting”: an approach to analysis of B lymphoid clonality in lymphoproliferative disorders.Br. J. Haematol., 1991,77, 274–281.

    Article  PubMed  CAS  Google Scholar 

  40. DEANE M., MC CARTHY K. P., WIEDEMANN L. M., NORTON J. D. — An improved method for detection of B-lymphoid clonality by polymerase chain reaction.Leukemia, 1991,5, 726–730.

    PubMed  CAS  Google Scholar 

  41. GRIBBEN J. G., NADLER L. M. — Detection of minimal residual disease in patients with lymphomas using polymerase chain reaction. Important Advances in Oncology, edited by De Vitz V T, Hellman S, Rosenberg S A. J.B. Lippincott Company, Philadelphia 1994.

    Google Scholar 

  42. ROGERS B. B., ALPERT L. C., HINE E. A. S., BUFFONE G. J. — Analysis of DNA in fresh and fixed tissue by the polymerase chain reaction.Am. J. Pathol., 1990,136, 541–548.

    PubMed  CAS  Google Scholar 

  43. ALGARA P., MARTINEZ P., SANCHEZ L.,et al. — The detection of B-cell monoclonal populations by polymerase chain reaction: Accuracy of approach and application in gastric endoscopic specimens.Hum. Pathol., 1993,24, 1184–1188.

    Article  PubMed  CAS  Google Scholar 

  44. SUKPANICHNANT S., VNENCAK-JONES C. L., MC CURLEY T. L. — Determination of B-cell clonality in paraffin-embedded endoscopic biopsy specimens of abnormal lymphocytic infiltrates and gastrointestinal lymphoma by polymerase chain reaction.Am. J. Clin. Pathol., 1994,102, 299–305.

    PubMed  CAS  Google Scholar 

  45. SLACK D. N., MC CARTHY K. P., WIEDEMANN L. M., SLOANE J. P. — Evaluation of sensitivity, specificity, and reproducibility of an optimized method for detecting clonal rearrangements of immunoglobulin and T-cell receptor genes in formalin-fixed, paraffin-embedded sections.Diagn. Mol. Pathol., 1993,2, 223–232.

    PubMed  CAS  Google Scholar 

  46. TRAINOR K. J., BRISCO M. J., STORY C. J., MORLEY A. A. — Monoclonality in B-lymphoproliferative disorders detected at the DNA level.Blood, 1990,75, 2220–2222.

    PubMed  CAS  Google Scholar 

  47. STELTLER-STEVENSON M., RAFFELD M., COHEN P., COSSMAN J. — Detection of occult follicular lymphoma by specific DNA amplification.Blood, 1988,72, 1822–1825.

    Google Scholar 

  48. CHAN W. C., GREINER T. C. — Diagnosis of lymphomas by the polymerase chain reaction.Am. J. Clin. Pathol., 1990,102, 273–274.

    Google Scholar 

  49. KWOK S., HIGUCHI R. — Avoiding false positives with PCR.Nature, 1989,339, 237–238.

    Article  PubMed  CAS  Google Scholar 

  50. MAPSTONE N. P., QUIRKE P. — Molecular biology and infections of the gut.Gut, 1992,33, 1441–1443.

    Article  PubMed  CAS  Google Scholar 

  51. DISS T.C., PENG H., WOTHERSPOON A.C., ISAACSON P.G., PAN L. — Detection of monoclonality in low-grade B-cell lymphomas using the polymerase chain reaction is dependent on primer selection and lymphoma type.J. Pathol., 1993,169, 291–295.

    Article  PubMed  CAS  Google Scholar 

  52. WOTHERSPOON A.C., DOGLIONI C., DISS T.C., MOSCHINI A., DE BONI M., ISAACSON P.G. — Regression of primary low-grade B-cell gastric lymphoma of mucosa-associated lymphoid tissue after eradication of Helicobacter pylori.Lancet, 1993,342, 571–574.

    Article  Google Scholar 

  53. KNOWLES D., HALPER J. P., JAKOBIEC F. A. — The immunologic characterization of 40 extranodal lymphoid infiltrates. Usefulness in distinguishing between benign pseudolymphoma and malignant lymphoma.Cancer, 1982,49, 2321–2335.

    Article  PubMed  Google Scholar 

  54. EIMOTO T., FUTAMI K., NAITO H., TAKESHITA M., KIKUCHI M. — Gastric pseudolymphoma with monotypic cytoplasmic immunoglobulin.Cancer, 1985,55, 788–793.

    Article  PubMed  CAS  Google Scholar 

  55. WOLF J. A., SPJUT H. J. — Focal lymphoid hyperplasia of the stomach preceding gastric lymphoma: Case report and review of the literature.Cancer, 1981,48, 2518–2523.

    Article  PubMed  Google Scholar 

  56. SIGAL H.S., SAUL S.H., AUERBACH H.E., RAFFENSPERGER E., KANT J.A., BROOKS J.J. — Gastric small lymphocytic proliferation with immunoglobulin gene rearrangement in pseudolymphoma versus lymphoma.Gastroenterology, 1989,97, 195–201.

    PubMed  CAS  Google Scholar 

  57. SPENCER J., DISS T.C., ISAACSON P.G. — Primary B-cell gastric lymphoma. A genotypic analysis.Am. J. Pathol. 1989,135, 557–564.

    PubMed  CAS  Google Scholar 

  58. SWEENEY J.F., MUUS C., McKEOWN P.P., ROSEMURGY S. — Gastric pseudolymphoma. Not necessarily a benign lesion.Dig. Dis. Sci., 1992,37, 939–945.

    Article  PubMed  CAS  Google Scholar 

  59. HEY M.M., FELLER A.C., KIRCHNER T., MÜLLER J., MÜLLER-HERMELINK H. — Genomic Analysis of T-cell Receptor and Immunoglobulin Antigen Receptor Genes and Breakpoint Cluster Regions in Gastrointestinal Lymphomas.Hum. Pathol., 1990,21, 1283–1287.

    Article  PubMed  CAS  Google Scholar 

  60. FEND F., SCHWAIGER A., WEYRER K.,et al. — Early diagnosis of gastric lymphoma: gene rearrangement analysis of endoscopic biopsy samples.Leukemia, 1994,8, 35–39.

    PubMed  CAS  Google Scholar 

  61. ONO H., KONDO H., SAITO D.et al. — Rapid diagnosis of gastric malignant lymphoma from biopsy specimens: Detection of immunoglobulin heavy chain rearrangement by polymerase chain reaction.Jpn. J. Cancer Res., 1993,84, 813–817.

    PubMed  CAS  Google Scholar 

  62. SANCHEZ L., ALGARA P., VILLUENDAS R.et al. — B-cell clonal detection in gastric low-grade lymphomas and regional lymph nodes: an immunohistologic and molecular study.Am. J. Gastroenterol., 1993,88, 413–419.

    PubMed  CAS  Google Scholar 

  63. DISS T.C., PENG H., WOTHERSPOON A.C., PAN L., SPEIGHT P.M., ISAACSON P.G. — Brief report: A single neoplastic clone in sequential biopsy specimens from a patient with primary gastric-mucosa-associated lymphoid-tissue lymphoma and Sjögren syndrome.N. Engl. J. Med., 1993,329, 172–175.

    Article  PubMed  CAS  Google Scholar 

  64. MC CORMICK C., PHILP E., MANSI J., LIVNI N., MC CARTHY K. — Clonal analysis of three morphologically distinct lymphomas occurring in the same patient.J. Clin. Pathol., 1994,47, 1038–1042.

    Article  CAS  Google Scholar 

  65. KAWAMATA N., MIKI T., FUKUDA T.et al. — Determination of a common clonal origin of gastric and pulmonary mucosa-associated lymphoid tissue lymphomas presenting five years apart.Internal Medicine, 1995,34, 220–223.

    Article  PubMed  CAS  Google Scholar 

  66. MONTALBAN C., MANZANAL A., CASTRILLO J.M., ESCRIBANO L., BELLAS C. — Low grade gastric B-cell MALT lymphoma progressing into high grade lymphoma. Clonal identity of the two stages of the tumour, unusual bone involvement and leukaemic dissemination.Histopathol., 1995,27, 89–91.

    Article  CAS  Google Scholar 

  67. LASOTA J., HYJEK E., KOO C.H., BLONSKI J., MIETTINEN M. — Cytokeratin-positive large-cell lymphomas of B-cell lineage. A study of five phenotypically unusual cases verified by polymerase chain reaction.Am. J. Surg. Pathol., 1996,20, 346–354.

    Article  PubMed  CAS  Google Scholar 

  68. GALTON D. A. G., CATOVSKY D., WILTSHAW E. — Clinical spectrum of lymphoproliferative diseases.Cancer, 1978,42, 901–910.

    Article  PubMed  CAS  Google Scholar 

  69. FISHLEDER A., TUBBS R., HESSE B., LEVINE H. — Uniform detection of immunoglobulin-gene rearrangement in benign lymphoepithelial lesions.N. Engl. J. Med., 1987,316, 1118–1121.

    Article  PubMed  CAS  Google Scholar 

  70. TAKANO Y., KATO Y., SATO Y., OKUDAIRA M. — Clonal Ig-gene rearrangement in some cases of gastric RLH detected by PCR method.Path. Res. Pract., 1992,188, 973–980.

    PubMed  CAS  Google Scholar 

  71. WOTHERSPOON A.C., DISS T.C., PAN L., SAVIO A., ISAACSON P.G. — Diagnosis of low grade MALT lymphoma in gastric biopsies: histology, PCR or both?J. Pathol., 1994,173, 151A.

  72. CALVERT R., RANDERSON J., EVANS P.,et al. — Genetic abnormalities during transition from Helicobacter-pylori-associated gastritis to low-grade MALToma.Lancet, 1995,345, 26–27.

    Article  PubMed  CAS  Google Scholar 

  73. CALVERT R. J., EVANS P. A. S., RANDERSON J. A., JACK A. S., MORGAN G. J., DIXON M. F. — The significance of B-cell clonality in gastric lymphoid infiltrates.J. Pathol., 1996,180, 26–32.

    Article  PubMed  CAS  Google Scholar 

  74. FERRACCIOLI G. F., SORRENTINO D., DE VITA S.,et al. — B cell clonality in gastric lymphoid tissue of patients with Sjögren’s syndrome.Ann. Rheum. Dis., 1996,55, 311–316.

    Article  PubMed  CAS  Google Scholar 

  75. HSI E. D., GREENSON J. K., SINGLETON T. P., SIDDIQUI J., SCHNITZER B., ROSS C. W. — Detection of immunoglobuline heavy chain gene rearrangement by polymerase chain reaction in chronic active gastritis associated with Helicobacter pylori.Hum. Pathol., 1996,27, 290–296.

    Article  PubMed  CAS  Google Scholar 

  76. SAVIO A., FRANZIN G., WOTHERSPOON A.C.et al. — Diagnosis and posttreatment follow-up of Helicobacter Pylori-positive gastric lymphoma of mucosa-associated lymphoid tissue: histology, polymerase chain reaction, of both?Blood, 1996,87, 1255–1260.

    PubMed  CAS  Google Scholar 

  77. SORRENTINO D., FERRACIOLI G. F., DE VITA S.et al. — B-cell clonality and infection with Helicobacter pylori: implications for development of gastric lymphoma.Gut, 1996,38, 837–840.

    Article  PubMed  CAS  Google Scholar 

  78. SEVERSON R.K., DAVIS S. — Increasing incidence of primary gastric lymphoma.Cancer, 1990,66, 1283–1287.

    Article  PubMed  CAS  Google Scholar 

  79. SORRENTINO D., FERRACIOLI G. F., LABOMBARDA A.,et al. — Helicobacter pylori, gastric MALT and B-cell clonality.Clinical Experimental Rheumatology, 1996,14 (Suppl 14), 51–54.

    Google Scholar 

  80. ISAACSON P. G. — Recent developments in our understanding of gastric lymphomas.Am. J. Surg. Pathol., 1996,20 (Suppl. 1), S1-S7.

    PubMed  Google Scholar 

  81. WOTHERSPOON A.C., ORTIZ-HIDALGO C., FALZON M.R., ISAACSON P.G. — Helicobacter pylori-associated gastritis and primary B-cell gastric lymphoma.Lancet, 1991,338, 1175–1176.

    Article  PubMed  CAS  Google Scholar 

  82. EIDT S., STOLTE M., FISCHER R. — Helicobacter pylori gastritis and primary gastric non-Hodgkin’s lymphomas.J. Clin. Pathol., 1994,47, 436–439.

    Article  PubMed  CAS  Google Scholar 

  83. DOGLIONI C., WOTHERSPOON A.C., MOSCHINI A., DE BONI M., ISAACSON P. — High incidence of primary gastric lymphoma in northeastern Italy.Lancet, 1992,339, 834–835.

    Article  PubMed  CAS  Google Scholar 

  84. WOTHERSPOON A.C., DOGLIONI C., DISS T.C., MOSCHINI A., DE BONI M., ISAACSON P.G. — Regression of primary low-grade B-cell gastric lymphoma of mucosa-associated lymphoid tissue after eradication of Helicobacter pylori.Lancet, 1993,342, 571–574.

    Article  Google Scholar 

  85. STOLTE M., EIDT S. — Healing gastric MALT lymphomas by eradicating H pylori?Lancet, 1993,342, 568.

    Article  PubMed  CAS  Google Scholar 

  86. OKAZAKI K., MORITA M., YAMAMOTO Y. — Gene rearrangements, Helicobacter pylori, and gastric MALT lymphoma.Lancet, 1994,343, 1636.

    Article  PubMed  CAS  Google Scholar 

  87. WEBER D.M., DIMOPOULOS M.A., ANANDU D.P., PUGH W.C., STEINBACH G. — Regression of gastric lymphoma of mucosa-associated lymphoid tissue with antibiotic therapy for Helicobacter pylori.Gastroenterology, 1994,107, 1835–1838.

    PubMed  CAS  Google Scholar 

  88. WOTHERSPOON A.C., DOGLIONI C., DE BONI M., SPENCER J., ISAACSON P.G. — Antibiotic treatment for low-grade gastric MALT lymphoma.Lancet, 1994,343, 1503.

    Article  PubMed  CAS  Google Scholar 

  89. BAYERDÖRFFER E., NEUBAUER A., RUDOLPH B.et al. — Regression of primary gastric lymphoma of mucosa-associated lymphoid tissue type after cure of Helicobacter pylori infection.Lancet, 1995,345. 1591–1594

    Article  PubMed  Google Scholar 

  90. ROGGERO E., ZUCCA E., PINOTTI G.et al. — Eradication of Helicobacter pylori infection in primary low-grade gastric lymphoma of mucosa-associated lymphoid tissue.Ann. Intern. Med., 1995,122, 767–769.

    PubMed  CAS  Google Scholar 

  91. PESCATORE P., HEINE M., MANEGOLD B.C. — Cure of gastric lymphomas with antibiotics.Gastroenterology, 1995,109, 334–335.

    Article  PubMed  CAS  Google Scholar 

  92. RELMAN D.A., SCHMIDT T.M., MACDERMOTT R.P., FALKOW S — Identification of the uncultured bacillus of Whipple’s disease.N. Engl. J. Med., 1992,327, 293–301.

    Article  PubMed  CAS  Google Scholar 

  93. LAGE A.P., GODFROID E., FAUCONNIER A.et al. — Diagnosis of Helicobacter pylori infection by PCR: Comparison with other invasive techniques and detection of CagA gene in gastric biopsy specimens.J. Clin. Microbiol., 1995,33, 2752–2756.

    PubMed  CAS  Google Scholar 

  94. BLUM H. E. — Molekularbiologie: Einsatz in der Hepato-Gastroenterologie.Leber, Magen, Darm, 1993,23, 144–149.

    PubMed  CAS  Google Scholar 

  95. TAHARA E. — Molecular biology of gastric cancer.World. J. Surgery, 1995,19, 484–490.

    Article  CAS  Google Scholar 

  96. GOLDSTONE A. R., QUIRKE P., DIXON M. F. — Helicobacter pylori infection and gastric cancer.J. Pathol., 1996,179, 129–137.

    Article  PubMed  CAS  Google Scholar 

  97. TSUJI S., KAWANO S., TSUJII M., FUSAMOTO H., KAMADA T. — Roles of hepatocyte growth factor and its receptor in gastric mucosa. A cell biological and molecular biological study.Dig. Dis. Sci., 1995,40, 1132–1139.

    Article  PubMed  CAS  Google Scholar 

  98. NAKAMURA R. M. — Overview and principles of in-situ hybridization.Clin. Biochem., 1990,23, 255–259.

    Article  PubMed  CAS  Google Scholar 

  99. O’LEARY J. J., CHETTY R., GRAHAM A. K., MCGEE J. O’D. — In situ PCR: pathologist’s dream or nightmare.J. Pathol., 1996,178, 11–20.

    Article  PubMed  CAS  Google Scholar 

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  1. A. Driessen

    Present address: Department of Pathology, U.Z. Sint-Rafaël, Minderbroedersstraat 12, 3000, Leuven, Belgium

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  1. Department of Pathology, University Hospitals, K.U. Leuven, Belgium

    A. Driessen, N. Ectors, S. Pittaluga, M. Maes, J. Delabie & C. De Wolf-Peeters

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Driessen, A., Ectors, N., Pittaluga, S. et al. La technique de PCR est une méthode plus précise pour déterminer la monoclonalité des lymphomes gastriques. Acta Endosc 27, 101–115 (1997). https://doi.org/10.1007/BF02968931

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