Abstract
Background
In this study we activated breast cancer-specific cytotoxic T lymphocytes (CTL) from regional lymph node lymphocytes (RLNL) of HLA-A2-positive patients with breast cancer.
Melthods
Freshly isolated RLNL were stimulated with solid phase anti-CD3 monoclonal antibody followed by expansion with recombinant interleukin-2. Subsequently, the RLNL were stimulated with an irradiated HLA 0201 breast cancer cell line, MCF-7, at a responder/stimulator ratio of 10/1 once a week for 2 weeks.
Results
The cultured RLNL exhibited specific lysis against MCF-7 in all 5 HLA-A2-positive patients tested, but not in 2 HLA-A2-negative patients. Cytotoxicity against MCF-7 was substantially inhibited by addition of anti-HLA-A2 mAb. In 3 of 5 HLA-A2-positive patients, anti-MCF-7 CTL also exhibited a substantial level of reactivity against PC-9, an HLA-A0206-positive lung adenocarcinoma cell line. Conversely, anti-PC-9-specific CTL were inducible by multiple stimulations of RLNL with PC-9 cells in 2 of 3 patients.
Conclusions
These results suggest that several common tumor antigens might exist among HLA-A2-positive breast cancers, some of which may be shared with lung adenocarcinomas.
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Abbreviations
- CTL:
-
Cytotoxic T lymphocyte
- RLNL:
-
Regional lymph node lymphocyte
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Eifuku, R., Yoshino, I., Imahayashi, S. et al. Induction of tumor-specific cytotoxic T lymphocytes from regional lymph node lymphocytes of human breast cancer. Breast Cancer 5, 367–373 (1998). https://doi.org/10.1007/BF02967433
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DOI: https://doi.org/10.1007/BF02967433