Skip to main content
SpringerLink
Log in

Flow cytometric analysis to assess the malignant potential of phyllodes tumor

Breast Cancer volume 1, pages 139–143 (1994)Cite this article

Abstract

To evaluate DNA content as a prognostic indicator in phyllodes tumor of the breast, flow cytometric DNA analyses were performed retrospectively in 13 patients who were selected, based on the availability of paraffin-embedded tumor specimens. All were women with an average age of 41. 9 years and a mean follow-up of 51.2 months. Pathologically, 3 patients (23.0%) had malignant tumor and 10 (77.7%) had benign lesions. The patients with malignant tumors tended to be older and had larger tumors. Aneuploidy was seen in one patient with malignant tumor (7.7%), with the S-phase fraction ranging from 0.8 to 12.6. This patient died of lung metastases 6 months after mastectomy. One benign tumor showing diploid, developed local recurrence 100 months after lumpectomy. DNA stem cell lines were not detected in the only patient with aneuploid tumor. There was no significant correlation between DNA content and local recurrence. In this series, the number of patients was too small to draw the certain conclusions from data. However, it appears that, compared to diploid tumors, aneuploid tumor are more often malignant and have poorer prognosis. Our study indicates that DNA content has little prognostic value, DNA aneuploidy appears to be associated with a poor prognosis.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

USD 39.95

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Abbreviations

HPF:

High power field

SPF:

S-phase fraction

References

  1. Keelan PA, Myers JL, Wold LE,et al: Phyllodes tumor; Clinicopathologic review of 60 patients and flow cytometric analysis in 30 patients.Hum Pathol 23:1048–1054, 1992.

    Article  PubMed  CAS  Google Scholar 

  2. Tsuchiya A, Hoshi K, Ando Y,et al: Relationship between histopathological findings and nuclear DNA content of malignant phyllodes tumors of the breast.Pathol Clin Med 11:215–220, 1992 (in Japanese with English summary).

    Google Scholar 

  3. Hedley DW, Friedlander ML, Taylor IW,et al: Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry.J Histochem Cytochem 31:1333–1335, 1983.

    PubMed  CAS  Google Scholar 

  4. Bilchert-Toft M, Hansen I, Hansen O,et al: Clinical course of cystosarcoma phyllodes related to histological appearance.Surg Gynecol Obstet 140:929- 932, 1975.

    Google Scholar 

  5. Contarini O, Urdaneta L, Hagan W,et al: Cystosarcoma phyllodes of the breast; A new therapeutic proposal.Am Surg 48:157–166, 1982.

    PubMed  CAS  Google Scholar 

  6. Kessinger A, Foley J, Lemon H,et al: Metastatic cystosarcoma phyllodes; A case report and a review of the literature.J Surg Oncol 4:131–147, 1972.

    Article  PubMed  CAS  Google Scholar 

  7. Norris HJ, Taylor HB: Relationship of histologic features to behavior of cystosarcoma phyllodes; Analysis of ninety-four cases.Cancer 20:2090–2099, 1967.

    Article  PubMed  CAS  Google Scholar 

  8. Murad TM, Hines JR, Beal J,et al: Histological and clinical correlations of cystosarcoma phyllodes.Arch Pathol Lab Med 112:752–756, 1988.

    PubMed  CAS  Google Scholar 

  9. Pietruszka M, Barnes L: Cystosarcoma phyllodes; A clinicopathologic analysis of 42 cases.Cancer 41:1974–1983, 1978.

    Article  PubMed  CAS  Google Scholar 

  10. Hajdu SI, Espinosa MH, Robbins GF: Recurrent cystosarcoma phyllodes; A clinicopathologic study of 32 cases.Cancer 38:1402–1406, 1976.

    Article  PubMed  CAS  Google Scholar 

  11. Lindquist KD, van Heerden JA, Weiland LH,et al: Recurrent and metastatic cystosarcoma phyllodes.Am J Surg 144:341–343, 1982.

    Article  PubMed  CAS  Google Scholar 

  12. Ward RM, Evans HL: Cystosarcoma phyllodes; A clinicopathologic study of 26 cases. Cancer 58:2282- 2289, 1986.

    Article  PubMed  CAS  Google Scholar 

  13. Hart J, Layfield LJ, Trumbull WE,et al: Practical aspects in the diagnosis and management of cystosarcoma phyllodes.Arch Surg 123:1079–1083, 1988.

    PubMed  CAS  Google Scholar 

  14. Hart WR, Bauer RC, Oberman HA: Cystosarcoma phyllodes; A clinicopathologic study of twenty-six hypercellular periductal stromal tumors of the breast.Am J Clin Pathol 70:211–216, 1978.

    PubMed  CAS  Google Scholar 

  15. Thorud E, Fossa SD, Vaage S,et al: Primary breast cancer; Flow cytometric DNA pattern in relation to clinical and histopathologic characteristics.Cancer 57:808–811, 1986.

    Article  PubMed  CAS  Google Scholar 

  16. Abandowitz HM, Ow KT, Hardy D,et al: Relationship between flow cytometric parameters, steroid receptors and menopausal status in breast cancers.Oncology 44:24–29, 1987.

    Article  PubMed  CAS  Google Scholar 

  17. Dressier LG, Seamer LC, Owens MA,et al: DNA flow cytometry and prognostic factors in 1331 frozen breast cancer specimens.Cancer 61:420–427, 1988.

    Article  Google Scholar 

  18. Leyfield LJ, Hart J, Neuwirth H,et al: Relation between DNA ploidy and the clinical behavior of phyllodes tumors.Cancer 64:1486–1489, 1989.

    Article  Google Scholar 

  19. Palko MJ, Wang SE, Shackney SE,et al: Flow cytometric S-fraction as a predictor of clinical outcome in cystosarcoma phyllodes.Arch Pathol Lab Med 114:949–952, 1990.

    PubMed  CAS  Google Scholar 

  20. El-Nagger AK, Ro JY, Mclemore D,et al: DNA content and proliferative activity of cystosarcoma phyllodes of the breast.Am J Clin Pathol 93:480–485, 1990.

    Google Scholar 

  21. Tagawa Y, Yamaguchi H, Lin S,et al: Correlation between local recurrence and DNA ploidy on breast phyllodes tumor.J Jpn Soc Clin Surg 52:2540–2546, 1991 (in Japanese with English summary).

    Google Scholar 

  22. Chua CL, Thomas A: Cystosarcoma phyllodes tumors.Surg Gynecol Obstet 166:302–306, 1988.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. First Department of Surgery, Ryukyu University School of Medicine, 207 Uehara, Nishihara-cho, 903-01, Okinawa, Japan

    Shuji Tomita, Shigeru Deguchi, Toshiomi Kusano & Yoshihiro Muto

  2. Divisions of Surgery, Okinawa Prefectural Naha Hospital, Japan

    Nobumitsu Tamaki

  3. Divisions of Pathology, Okinawa Prefectural Naha Hospital, Japan

    Yoshiaki Nagamine

Authors
  1. Shuji Tomita
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shigeru Deguchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Toshiomi Kusano
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yoshihiro Muto
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Nobumitsu Tamaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yoshiaki Nagamine
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Tomita, S., Deguchi, S., Kusano, T. et al. Flow cytometric analysis to assess the malignant potential of phyllodes tumor. Breast Cancer 1, 139–143 (1994). https://doi.org/10.1007/BF02967044

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02967044

Key words

search

Navigation