To evaluate DNA content as a prognostic indicator in phyllodes tumor of the breast, flow cytometric DNA analyses were performed retrospectively in 13 patients who were selected, based on the availability of paraffin-embedded tumor specimens. All were women with an average age of 41. 9 years and a mean follow-up of 51.2 months. Pathologically, 3 patients (23.0%) had malignant tumor and 10 (77.7%) had benign lesions. The patients with malignant tumors tended to be older and had larger tumors. Aneuploidy was seen in one patient with malignant tumor (7.7%), with the S-phase fraction ranging from 0.8 to 12.6. This patient died of lung metastases 6 months after mastectomy. One benign tumor showing diploid, developed local recurrence 100 months after lumpectomy. DNA stem cell lines were not detected in the only patient with aneuploid tumor. There was no significant correlation between DNA content and local recurrence. In this series, the number of patients was too small to draw the certain conclusions from data. However, it appears that, compared to diploid tumors, aneuploid tumor are more often malignant and have poorer prognosis. Our study indicates that DNA content has little prognostic value, DNA aneuploidy appears to be associated with a poor prognosis.
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Keelan PA, Myers JL, Wold LE,et al: Phyllodes tumor; Clinicopathologic review of 60 patients and flow cytometric analysis in 30 patients.Hum Pathol 23:1048–1054, 1992.
Tsuchiya A, Hoshi K, Ando Y,et al: Relationship between histopathological findings and nuclear DNA content of malignant phyllodes tumors of the breast.Pathol Clin Med 11:215–220, 1992 (in Japanese with English summary).
Hedley DW, Friedlander ML, Taylor IW,et al: Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry.J Histochem Cytochem 31:1333–1335, 1983.
Bilchert-Toft M, Hansen I, Hansen O,et al: Clinical course of cystosarcoma phyllodes related to histological appearance.Surg Gynecol Obstet 140:929- 932, 1975.
Contarini O, Urdaneta L, Hagan W,et al: Cystosarcoma phyllodes of the breast; A new therapeutic proposal.Am Surg 48:157–166, 1982.
Kessinger A, Foley J, Lemon H,et al: Metastatic cystosarcoma phyllodes; A case report and a review of the literature.J Surg Oncol 4:131–147, 1972.
Norris HJ, Taylor HB: Relationship of histologic features to behavior of cystosarcoma phyllodes; Analysis of ninety-four cases.Cancer 20:2090–2099, 1967.
Murad TM, Hines JR, Beal J,et al: Histological and clinical correlations of cystosarcoma phyllodes.Arch Pathol Lab Med 112:752–756, 1988.
Pietruszka M, Barnes L: Cystosarcoma phyllodes; A clinicopathologic analysis of 42 cases.Cancer 41:1974–1983, 1978.
Hajdu SI, Espinosa MH, Robbins GF: Recurrent cystosarcoma phyllodes; A clinicopathologic study of 32 cases.Cancer 38:1402–1406, 1976.
Lindquist KD, van Heerden JA, Weiland LH,et al: Recurrent and metastatic cystosarcoma phyllodes.Am J Surg 144:341–343, 1982.
Ward RM, Evans HL: Cystosarcoma phyllodes; A clinicopathologic study of 26 cases. Cancer 58:2282- 2289, 1986.
Hart J, Layfield LJ, Trumbull WE,et al: Practical aspects in the diagnosis and management of cystosarcoma phyllodes.Arch Surg 123:1079–1083, 1988.
Hart WR, Bauer RC, Oberman HA: Cystosarcoma phyllodes; A clinicopathologic study of twenty-six hypercellular periductal stromal tumors of the breast.Am J Clin Pathol 70:211–216, 1978.
Thorud E, Fossa SD, Vaage S,et al: Primary breast cancer; Flow cytometric DNA pattern in relation to clinical and histopathologic characteristics.Cancer 57:808–811, 1986.
Abandowitz HM, Ow KT, Hardy D,et al: Relationship between flow cytometric parameters, steroid receptors and menopausal status in breast cancers.Oncology 44:24–29, 1987.
Dressier LG, Seamer LC, Owens MA,et al: DNA flow cytometry and prognostic factors in 1331 frozen breast cancer specimens.Cancer 61:420–427, 1988.
Leyfield LJ, Hart J, Neuwirth H,et al: Relation between DNA ploidy and the clinical behavior of phyllodes tumors.Cancer 64:1486–1489, 1989.
Palko MJ, Wang SE, Shackney SE,et al: Flow cytometric S-fraction as a predictor of clinical outcome in cystosarcoma phyllodes.Arch Pathol Lab Med 114:949–952, 1990.
El-Nagger AK, Ro JY, Mclemore D,et al: DNA content and proliferative activity of cystosarcoma phyllodes of the breast.Am J Clin Pathol 93:480–485, 1990.
Tagawa Y, Yamaguchi H, Lin S,et al: Correlation between local recurrence and DNA ploidy on breast phyllodes tumor.J Jpn Soc Clin Surg 52:2540–2546, 1991 (in Japanese with English summary).
Chua CL, Thomas A: Cystosarcoma phyllodes tumors.Surg Gynecol Obstet 166:302–306, 1988.
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Tomita, S., Deguchi, S., Kusano, T. et al. Flow cytometric analysis to assess the malignant potential of phyllodes tumor. Breast Cancer 1, 139–143 (1994). https://doi.org/10.1007/BF02967044