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Cytométrie de flux du DNA sur biopsies endoscopiques dans le dépistage de la dégénérescence de la colite ulcéreuse. Etude basée sur les caractères du DNA au niveau des tumeurs gastro-intestinales

DNA flow cytometry of endoscopic biopsies for detection of malignancy in ulcerative colitis. A study based on the DNA patterns of gastro-intestinal tumours

  • Published:
Acta Endoscopica

Résumé

Les caractères du DNA cellulaire de la muqueuse de 254 patients atteints de colite ulcéreuse ont été mesurés par méthode de cytométrie de flux, sur biopsies prélevées en cours de coloscopie. Les caractères de DNA ont été comparés avec ceux ďadénocarcinomes du tractus gastro-intestinal. Dans environ 10% des cas, un caractère DNA aneuploïde a été objectivé, indiquant une transformation maligne. La fréquence de la transformation aneuploïde augmente avec la durée de la maladie. A 1’opposé du caractère localisé des modifications aneuploïdes dans les cancers colorectaux, ces mêmes anomalies dans la muqueuse colitique sont souvent largement disséminées. Les résultats font apparaître la possibilité de combiner coloscopie et mesures quantitatives du DNA dans la détection précoce ďune dégénérescence maligne.

Summary

The cellular DNA content of mucosa in 254 patients with ulcerative colitis was measured by means of flow cytometry in biopsies taken at colonoscopy. The DNA patterns were compared to those of manifest adenocarcinomas of the gastro-intestinal tract. In approximately 10% of the cases an aneuploid DNA pattern was found indicating malignant transformation. The frequency of occurrence of aneuploidy increased with duration of disease. In contrast to the localized colorectal adenocarcinomas the aneuploid changes of the colitic mucosa were frequently widely spread. The results demonstrate the possibility of combining colonoscopy with quantitative DNA measurements for early detection of malignancy.

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Authors and Affiliations

  1. Department of Medical Radiobiology, Karolinska Institute, Stockholm, Sweden

    B. Tribukait & C. Hammarberg

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  1. B. Tribukait
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  2. C. Hammarberg
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Additional information

Tirés à part:Professor Bernhard Tribukait, Dept of Medical Radiobiology, Box 60204, S-104 01 Stockholm (Sweden).

Cette étude a été accomplie avec le soutien de la Stockholm Cancer Society, du Karolinska Institute et des Fondations Bergwall, Juhlin et Wallström.

This research was supported by grants from the Stockholm Cancer Society, the Karolinska Institute, the Bergwall, Juhlin, and Wallström Foundations.

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Tribukait, B., Hammarberg, C. Cytométrie de flux du DNA sur biopsies endoscopiques dans le dépistage de la dégénérescence de la colite ulcéreuse. Etude basée sur les caractères du DNA au niveau des tumeurs gastro-intestinales. Acta Endosc 15, 257–264 (1985). https://doi.org/10.1007/BF02966118

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  • DOI: https://doi.org/10.1007/BF02966118

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