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Biochemical pathogenesis of post-traumatic epilepsy

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An Erratum to this article was published on 01 October 1990

Abstract

Head trauma is often followed by epilepsy and may be related to the breakdown of red blood cells and hemoglobin within the CNS. Injection of hemoglobin or iron salts into the rat cortex is known to induce a chronic epileptic focus. We observed the formation of superoxide anion (O2) and hydroxyl radical (·OH) after ferric chloride injection into the rat cerebral cortex and suggest that these radicals, especially ·OH, may be responsible for the initiation of lipid peroxidation in neuronal membranes and for the accelerated production of guanidine compounds in the brain, which may in turn lead to epileptogenicity. Then, we found that treatment with epigallocatechin (EGC) or a phosphate diester of vitamins E and C (EPC), which are potent ·OH scavengers, significantly inhibited the formation of malondialdehyde and epileptic discharges in the iron-induced epileptic focus.

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References

  • Aisen, P. (1977) Some physiochemical aspects of iron metabolism.Ciba Foundation Symposium, 51, 1–14.

    Google Scholar 

  • Fukushima, M. (1987) Guanidino compounds in iron-induced epileptogenic foci of rats.Okayama Igakkai Zasshi, 99, 1–20 (in Japanese with English abstract).

    Google Scholar 

  • Grisar, T., and Delgado-Escueta, A. V. (1986) Astroglial contribution in human temporal lobe epilepsy: K+ activation of Na+,K+-ATPase in bulk isolated glial cells and synaptosomes.Brain Research, 364, 1–11.

    Article  PubMed  Google Scholar 

  • Grisar, T., Franck, G., and Delgado-Escueta, A. V. (1983) Glia contribution to seizure: K+ activation of Na+, K+-ATPase in bulk isolated glial cells and synaptosomes of epileptogenic cortex.Brain Research, 261, 75–84.

    Article  PubMed  Google Scholar 

  • Guidice, M. A., and Berchou, R. C. (1987) Posttraumatic epilepsy following head injury.Brain Injury, 1, 61–64.

    Article  PubMed  Google Scholar 

  • Gutteridge, J. M. C. (1986) Iron promoters of the Fenton reaction and lipid peroxidation can be released from hemoglobin by peroxides.FEBS Letters, 201, 291–295.

    Article  PubMed  Google Scholar 

  • Hiramatsu, M., Mori, A., and Kohno, M. (1984) Formation of peroxyl radical after FeCl3 injection into rat isocortex.Neurosciences, 10, 281–284.

    Google Scholar 

  • Hiramatsu, M., Fukushima, M., Kabuto, H., Edamatsu, R., and Mori, A. (1987) Amino acids, 5-hydroxytryptamine, and catecholamines in iron-induced epileptogenic foci of the rat.Advances in Epileptology, 16, 151–154.

    Google Scholar 

  • Hirayasu, Y., Morimoto, K., Otsuki, S., and Mori, A. (1989) Regional changes of guanidino compounds in the amygdala kindled rat brain.American Epilepsy Society (Abstract).

  • Janjua, N. A., Mori, A., and Hiramatsu, M. (1990) Increased aspartic acid release from the iron-induced epileptogenic focus.Epilepsy Research (in press).

  • Kalsbeek, W. D., McLaurin, R. L., Harris, B. S. H., and Miller, J. D. (1980) Report on the national head and spinal cord survey.Journal of Neurosurgery, 53, 519–531.

    Google Scholar 

  • Khochi, H. (1983) Brain active oxygen, free radicals, lipid peroxide and the redox state of glutathione in the Fe3+ induced epileptic focus of rat.Okayama Igakkai Zasshi, 95, 271–282 (in Japanese with English abstract).

    Google Scholar 

  • Matsumoto, M., Kobayashi, K., Kishikawa, H., and Mori, A. (1976) Convulsive activity of methylguanidine in cats and rabbits.IRCS Medical Science, 4, 65.

    Google Scholar 

  • Minotti, G., and Aust, S. (1987) An investigation into the mechanism of citrate-Fe2+-dependent lipid peroxidation.Free Radical Biology and Medicine, 3, 379–387.

    Article  PubMed  Google Scholar 

  • Mori, A. (1987) Biochemistry and neurotoxicology of guanidino compounds: History and recent advances.Pavlovian Journal of Biological Science, 22, 85–94.

    PubMed  Google Scholar 

  • Mori, A., and Hiramatsu, M. (1984) A role of radical reaction on the neuronal membrane in the seizure mechanism.Annual Report of Research “Cause and Treatment of Refractory Epilepsies” due to Grant from the Ministry of Health and Welfare of Japan, pp. 175–179.

  • Mori, A., Watanabe, Y., and Yokoi, I. (1986) Effect of ferric ion on activities of Na+,K+-ATPase in rat brain.IRCS Medical Science, 140, 707–708.

    Google Scholar 

  • Mori, A., Edamatsu, R., Kohno, M., and Ohmori, S. (1989a) A new hydroxyl radical scavenger: EPCK1.Neurosciences, 15 (in press).

  • Mori, A., Hiramatsu, M., Edamatsu, R., and Kohno, M. (1989b) Possible involvement of oxygen free radicals in the pathogenesis of post-traumatic epilepsy. In O. Hayaishiet al. (Eds.),Medical, Biochemical and Chemical Aspects of Free Radicals. Amsterdam: Elsevier, pp. 1249–1252.

    Google Scholar 

  • Nagase, S., Aoyagi, K., Narita, M., and Tojo, S. (1986) Active oxygen in methylguanidine synthesis.Nephron, 44, 299–306.

    Article  PubMed  Google Scholar 

  • O’Brien, P. J. (1969) Intracellular mechanisms for the decomposition of a lipid peroxide by metal ions, heme compounds and nucleophiles.Canadian Journal of Biochemistry, 47, 485–492.

    Article  PubMed  Google Scholar 

  • Ogata, K. (1986)Phosphoric Acid Diesters or Their Salts and Process for Producing the Same. U.S. Patent No. 4564,686.

  • Rapport, R. L., II, Harris, A. B., Friel, P. N., and Ojemann, G. A. (1975) Human epileptic brain: Na,K-ATPase activity and phenytoin concentration.Archives of Neurology, 32, 549–554.

    PubMed  Google Scholar 

  • Rosen, A. D., and Frumin, N. V. (1979) Focal epileptogenesis after intracortical hemoglobin injection.Experimental Neurology, 66, 277–284.

    Article  PubMed  Google Scholar 

  • Ruggles, K. H., Hansotia, P. L., and Ahmann, P. A. (1988) Head trauma and epilepsy.Wisconsin Medical Journal, 87, 16–18.

    PubMed  Google Scholar 

  • Sadrzadeh, S. M. H., Panter, S. S., Hallaway, P. E., and Eaton, J. W. (1984) Hemoglobin: A biological Fenton reagent.The Journal of Biological Chemistry, 259, 14354–14356.

    PubMed  Google Scholar 

  • Uchida, S., Edamatsu, R., Hiramatsu, M., Mori, A., Nonaka, G., Niwa, M. and Ozaki, M. (1987) Condensed tannins scavenge active oxygen free radicals.Medical Science Research, 15, 831–832.

    Google Scholar 

  • Ueno, I., Kohno, M., Yasuhisa, K., and Hirano, I. (1984) Quantitative determination of the superoxide radicals in the xanthine oxidase reaction by measurement of the electron spin resonance signal of the superoxide radical spin adduct of 5,5-dimethylpyrroline-l-oxide.Journal of Pharmacobio-Dynamics, 7, 563–569.

    PubMed  Google Scholar 

  • Wever, R., Qudega, B., and Van Gelder, B. F. (1973) Generation of superoxide radicals during the autoxidation of mammaliam oxyhemoglobin.Biochemica et Biophysica Acta, 302, 475–478.

    Google Scholar 

  • Willmore, L. J., and Rubin, J. J. (1981) Antiperoxidant pretreatment and iron-induced epileptiform discharges in the rate: EEG and histopathologic studies.Neurology, 31, 63–69.

    PubMed  Google Scholar 

  • Willmore, L. J., and Rubin, J. J. (1982) Formation of malonaldehyde and focal brain edema induced by subpial injection of FeCl2 into rat isocortex.Brain Research, 246, 113–119.

    Article  PubMed  Google Scholar 

  • Willmore, L. J., and Rubin, J. J. (1984) Effects of antiperoxidants on FeCl2-induced lipid peroxidation and focal edema in rat brain.Experimental Neurology, 82, 62–70.

    Article  Google Scholar 

  • Willmore, L. J., Sypert, G. W., Munson, J. B., and Hurd, R. W. (1978a) Chronic focal epileptiform discharges induced by injection of iron into rat and cat cortex.Science, 200, 150–153.

    Article  Google Scholar 

  • Willmore, L. J., Hura, R. W., and Supert, G. W. (1978b) Epileptiform activity initiated by pial iontophoresis of ferrous and ferric chloride on rat cerebral cortex.Brain Research, 152, 406–410.

    Article  PubMed  Google Scholar 

  • Willmore, L. J., Sypert, G. W., and Munson, J. B. (1978c) Recurrent seizures induced by cortical iron injection: A model of post-traumatic eplipsy.Annuals of Neurology, 4, 329–336.

    Article  Google Scholar 

  • Willmore L. J., Hiramatsu, M., Khochi, H., and Mori, A. (1983) Formation of superoxide radicals after FeCl3 injection into rat isocortex.Brain Research, 277, 293–296.

    Article  Google Scholar 

  • Willmore, L. J., Triggs, W. J., and Gray, J. D. (1986) The role of iron-induced hippocampal peroxidation in acute epileptogenesis.Brain Research, 382, 422–426.

    Article  PubMed  Google Scholar 

  • Wills, E. D. (1965) Mechanism of lipid peroxide formation in tissues: Role of metals and haematin proteins in the catalysis of the oxidation of unsaturated fatty acid.Biochimica et Biophysica Acta, 98, 238–251.

    PubMed  Google Scholar 

  • Yokoi, I., Kabuto, H., Yamamoto, M., and Mori, A. (1990) Effect of ferric ion and active oxygen scavenger on monoamine release in the striatum of the rat.Japanese Journal of Psychiatry and Neurology, 44 (in press).

  • Zhang, Z. H., Zuo, H., and Wu, X. R. (1989) Effects of lipid peroxidation of GABA uptake and release in iron-induced seizures.Chinese Medical Journal, 102, 24–27.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Institute for Neurobiology, Okayama University Medical School, 700, Okayama, Japan

    Akitane Mori M.D., PhD., Midori Hiramatsu Ph.D., Isao Yokoi M.D., Ph.D. & Rei Edamatsu B.P.

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  1. Akitane Mori M.D., PhD.
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  2. Midori Hiramatsu Ph.D.
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  3. Isao Yokoi M.D., Ph.D.
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  4. Rei Edamatsu B.P.
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Additional information

This study was supported by a grant-in-aid for scientific research from the Japanese Ministry of Education, Science, and Culture.

An erratum to this article is available at http://dx.doi.org/10.1007/BF02900705.

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Mori, A., Hiramatsu, M., Yokoi, I. et al. Biochemical pathogenesis of post-traumatic epilepsy. Pav. J. Biol. Sci. 25, 54–62 (1990). https://doi.org/10.1007/BF02964604

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