Skip to main content
SpringerLink
Log in

A study using an isotope probe comparing immunoassay with serology in detection of brucella abortus antibody

  • Published:
Irish Journal of Medical Science Aims and scope Submit manuscript

Summary

We report a comparison of radioimmunoassay with conventional serology in the detection of brucella abortus antibody from three laboratories. Overall agreement by Chi squared analysis is 5%. There are significant differences between laboratories and a significant number of sero negative suspect sera (from 20% – 60%) were positive by radio-immunoassay test. We suspect that conventional serology under-reports the incidence of antibody to brucella abortus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Cruckshank, R., Duguid, J. P., Marmion, B. P., Swain, R. H. A. 1973. Medical Microbiology—A Guide to the Laboratory Diagnosis and Control of Infection. 12th Edition. Vol. 1. Churchill Livingstone Publishers.

  • Cunningham, B., Hillary, I. 1985. Brucellosis in cattle and humans. I.M.J. 78, 202.

    Google Scholar 

  • Cunningham, B. 1968. The control and eradication of brucellosis Veterinary Record. 6 Jan. 1982, 7–11.

  • Devlin, J. G., Cunningham, B., Redington, F., Stephenson, M., Blake, P. 1982. Letter. Lancet 8299: II, 669.

    Google Scholar 

  • Kerr, W. R., McCaughey, W. J., Coghlan, J. D., Payne, D. J. H., Quaife, R. A., Robertson, L., Farrell, I. D. 1968. Techniques and interpretation in the serological diagnosis of brucellosis in man. J. Med. Microbiol. Vol. 1, 1981–193.

    Article  Google Scholar 

  • Magee, J. T. 1980. An enzyme-labelled immunosorbent assay for brucella abortus antibodies. J. Med. Microbiol. Vol. 13, 167–172.

    PubMed  CAS  Google Scholar 

  • Mondell, Douglas, Bennett. 1979. Principles of Infectious Disease. Wiley Medical Publications 1772–1784.

  • McConahey, P. G., Dixon, F. J. 1966. A Method of Trace lodination of Proteins for Immunological Studies. Int. Archives of Allergy and Applied Immunology. 29, 185.

    CAS  Google Scholar 

  • Parratt, D., Nielsen, K. H., White, R. G., Payne, D. J. H. 1977. Radio-immunoassay of IgM, IgG and IgA brucella antibodies. Lancet. May 21, 1075–1078.

    Google Scholar 

  • White, R. G. 1978. Immunoglobulin profiles of the chronic antibody response: discussion in relation to brucellosis infections. Postgraduate Med. J. 54, 595–602.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. St. Laurence’s Hospital, Dublin 7

    J. G. Devlin, F. Redington & M. Stephenson

Authors
  1. J. G. Devlin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. F. Redington
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Stephenson
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Devlin, J.G., Redington, F. & Stephenson, M. A study using an isotope probe comparing immunoassay with serology in detection of brucella abortus antibody. I.J.M.S. 155, 349–352 (1986). https://doi.org/10.1007/BF02960716

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02960716

Keywords

Search

Navigation