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A quantitative study of the perikaryon and the basal dendritic tree in rat hippocampus (CA1) pyramids following different experimental procedures

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Abstract

A quantitative study of perikaryon and basal dendritic branching of hippocampal (CA1) neurons in male Wistar rats, in three experimental groups is reported. The groups of animals were exposed to treatment with either a tricyclic antidepressant, or a saline control injection or no handling. The hippocampal tissue was studied following Golgi-Kopsch impregnation and comparative measurements made using semi-automatic image analysis and light microscopy. Measurements of the extent of basal dendritic branching showed a significant difference (p<0.01) between the saline-injected and unhandled control groups. The drug-injected and saline-injected groups had similar values although the drug-injected group did not prove to be significantly different from the unhandled controls. No statistical difference was noted in the perikaryon size of the three experimental groups. These results are discussed with reference to the effects of sensory stimulation on neuronal plasticity.

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References

  1. Feldman, M. L. Dendritic changes in aging rat brain: Pyramidal cell dendrite length and ultrastructure. In: AgeingBrain and Senile Dementia. Ed. K. Nandy, I. Sherwin, New York, Plenum Press, 1977: pp. 23–37.

    Google Scholar 

  2. Ferrer, I., Guionnet, N., Cruz-Sanchez, F., Tunon, T. Neuronal alterations in patients with senile dementia: a Golgi study on biopsy samples. Neurosci. Lett. 1990:114,11–16.

    Article  PubMed  CAS  Google Scholar 

  3. Mussa, G. C., Zaffaroni, M., Mussa, F. Thyroid and growth: thyroid hormones and development of the nervous system. J. Endocrinol. Invest. 1989:12,85–89.

    PubMed  CAS  Google Scholar 

  4. Woolley, C. S., Gould, E., Frankfurt, M., McEwen, B. S. Naturally occurring fluctuation in dendritic spine density on adult hippocampal pyramidal neurons. J. Neurosci. 1990:10, 4035–4039.

    PubMed  CAS  Google Scholar 

  5. Horner, C. H., O’Regan, M., Arbuthnott, E. Neural plasticity of the hippocampal (CA1) pyramidal cell- quantitative changes in spine density following handling and injection for drug testing. J. Anat. 1991: 174, 229–238.

    PubMed  CAS  Google Scholar 

  6. Bryan, G. K., Riesen, A H. Deprived somatosensory-motor experiences in stumptailed monkey neocortex: dendritic spine density and dendritic branching of layer 111 B pyramidal cells. J. Comp. Neurol. 1989:286, 208–217.

    Article  PubMed  CAS  Google Scholar 

  7. Schapiro, S., Vukovich, K. R. Early experience effects upon cortical dendrites: a proposed model for development. Science 1970: 167,292–294.

    Article  PubMed  CAS  Google Scholar 

  8. Pentney, R. J. Quantitative analysis of ethanol effects on Purkinje cell dendritic tree. Brain Res. 1982: 249, 397–401.

    Article  PubMed  CAS  Google Scholar 

  9. Feldman, M. L. Ageing changes in the morphology of cortical dendrites. In: Neurobiology of Ageing. Ed. R. D. Terry, S. Gershon, New York: Raven Press, 1976: pp. 211–227.

    Google Scholar 

  10. McConnell, P., Berry, M. The effects of malnutrition on Purkinje cell dendritic growth in the rat. J. Comp. Neurol. 1978: 177, 159–172.

    Article  PubMed  CAS  Google Scholar 

  11. Nunzi, M. G., Milan, F., Guidolin, D., Polato, P., Toffano, G. Effects of phosphatidylserine administration of age-related structural changes in the rat hippocampus and septal complex. Pharmacopsychiatry 1989: 22, 125–128.

    Article  PubMed  Google Scholar 

  12. Uylings, H. B. M., Kuypers, K., Diamond, M. C., Veltman, W. A. M. Effects of differential environments on plasticity of dendrites of cortical pyramidal neurons in adult rats. Exp. Neurol. 1978: 62, 658–677.

    Article  PubMed  CAS  Google Scholar 

  13. Volkmar, F. R., Greenough, W. T. Rearing complexity affects branching of dendrites in the visual cortex of the rat. Science 1972: 176,1445–1447.

    Article  PubMed  CAS  Google Scholar 

  14. Greenough, W. T., Volkmar, F. R. Pattern of dendritic branching in occipital cortex of rats reared in complex environments. Exp. Neurol. 1973: 40,491–504.

    Article  PubMed  CAS  Google Scholar 

  15. Purves, D., Hadley, R. D. Changes in the dendritic branching of adult mammalian neurones revealed by repeated imaging in situ. Nature 1985: 315,404–406.

    Article  PubMed  CAS  Google Scholar 

  16. Juraska, J.M., Fitch, J. M., Washburne, D. L. The dendritic morphology of pyramidal neurons in the rat hippocampal CA3 area. II Effects of gender and environment. Brain Res. 1989:479,115–119.

    Article  PubMed  CAS  Google Scholar 

  17. Juraska, J. M. Sex differences in dendritic response to differential experience in the rat visual cortex. Brain Res. 1984: 295, 29–34.

    Article  Google Scholar 

  18. Hillman, D. E., Chen, S. Vulnerability of cerebellar development in malnutrition -1. Quantitation of layer volume and neuron numbers. Neuroscience 1981: 6, 1249–1262.

    Article  PubMed  CAS  Google Scholar 

  19. Hollister, L. E. Clinical Pharmacology of Psychotherapeutic Drugs (2nd Ed.) New York: Churchill Livingstone, 1983: pp.94

    Google Scholar 

  20. Riley, J. N. A reliable Golgi-Kopsch modification. Brain Res. Bull. 1979: 4, 127–129.

    Article  PubMed  CAS  Google Scholar 

  21. Snedecor, G. W., Cochran, W. G. Statistical Methods (6th Ed.) Ames, Iowa: Iowa State Univ. Press, 1973.

    Google Scholar 

  22. Bradley, P., Berry, M. Quantitative effects of climbing fibre deafferentation on the adult Purkinje cell dendritic tree. Brain Res. 1976: 112, 133–140.

    Article  PubMed  CAS  Google Scholar 

  23. Globus, A., Rosenzweig, M. R., Bennett, E. L., Diamond, M. C. Effects of differential experience on dendritic spine counts in rat cerebral cortex. J. Comp. Physiol. Psychol. 1973:82,175–181.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Anatomy, Trinity College, Dublin 2

    C. H. Horner

  2. Department of Statistics, Trinity College, Dublin 2

    M. O’Regan

  3. Department of Physiology, Trinity College, Dublin 2

    E. Arbuthnott

Authors
  1. C. H. Horner
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  2. M. O’Regan
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  3. E. Arbuthnott
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Horner, C.H., O’Regan, M. & Arbuthnott, E. A quantitative study of the perikaryon and the basal dendritic tree in rat hippocampus (CA1) pyramids following different experimental procedures. I.J.M.S. 162, 258–262 (1993). https://doi.org/10.1007/BF02957574

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