Skip to main content
SpringerLink
Log in

Follistatin has characteristics of a primary response gene in porcine granulosa cells

  • Papers
  • Published:
Endocrine Aims and scope Submit manuscript

Abstract

To explore the regulation of follistatin gene expression, porcine granulosa cells were incubated with the translational inhibitor, cycloheximide (CHX), for periods from 6–24 h. This resulted in a 3 to 10-fold increase in follistatin mRNA accumulation compared to vehicle treated control cultures. At 20 h, CHX augmented the follicle stimulating hormone (FSH) induced stimulation of follistatin mRNA accumulation by a mean of more than sixfold. Over 6 h, CHX elevated follistatin mRNA abundance twofold, while epidermal growth factor (EGF) increased the message threefold. CHX in the presence of EGF produced an effect additive to the EGF response. Results in the longer term differed, as pretreatment of granulosa cells with CHX for 20 h suppressed the induction of follistatin gene expression by both EGF and phorbol 12-myristate-13-acetate. By blockade of transcription with Actinomyocin D, an estimate of the half-life of follistatin mRNA between 4 and 8 h was made. Half-life did not appear to be affected by the CHX suppression of protein translation. From the observations of the occurrence of follistatin gene expression independent of protein synthesis, superinduction in the presence of CHX and FSH, and the interactions between CHX and EGF, it is concluded that follistatin is a primary response gene in porcine granulosa cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Alliston, T., Firestone, G.L., Simmons, D.L. & Richards, J.S. (1994). Prog. 10th Ov. Workshop Serono Symp. Ann Arbour MI (Abst).

  • Chedrese, P.J., Luu The, V., Labrie, F., Juorio, A.V. & Murphy, B.D. (1990).Endocrinology,126, 2228–2230.

    PubMed  CAS  Google Scholar 

  • Delidow, B.C., White, B.A. & Peluso, J.J. (1990).Endocrinology,126, 2302–2306.

    PubMed  CAS  Google Scholar 

  • De Paolo, L.V., Bicsak, T.A., Erickson, G.F., Shimasaki, S. & Ling, N. (1991).Proc. Soc. Exptl. Biol. Med.,198, 500–512.

    Google Scholar 

  • Esch, F.S., Shimasaki, S., Mercado, M., Cooksey, K., Ling, N., Ying, S., Ueno, N. & Guillemin, R. (1987).Mol. Endocrinol.,1, 849–855.

    PubMed  CAS  Google Scholar 

  • Findlay, J.K. (1993).Biol. Reprod.,48, 15–23.

    Article  PubMed  CAS  Google Scholar 

  • Fletcher, B.S., Lim, R.W. Varnum, B.C., Kujubu, D.A., Koski, R.A. & Herschman, H.R. (1991).J. Biol. Chem.,266, 14511–14518.

    PubMed  CAS  Google Scholar 

  • Gospodarowicz, D. & Bialecki, H. (1978).Endocrinology,104, 757–764.

    Google Scholar 

  • Esch, F.S., Shimasaki, S., Mercado, M., Cooksey, K., Ling, N., Ying, S., Ueno, N. & Guillemin, R. (1987).Mol. Endocrinol.,1, 849–855.

    Article  PubMed  CAS  Google Scholar 

  • Findlay, J.K. (1993).Biol. Reprod.,48, 15–23.

    Article  PubMed  CAS  Google Scholar 

  • Fletcher, B.S., Lim, R.W. Varnum, B.C., Kujubu, D.A., Koski, R.A. & Herschman, H.R. (1991).J. Biol. Chem.,266, 14511–14518.

    PubMed  CAS  Google Scholar 

  • Gospodarowicz, D. & Bialecki, H. (1978).Endocrinology,104, 757–764.

    Google Scholar 

  • Hemmati-Brivaniou, A., Kelly, O.G. & Melton, D.A. (1994).Cell,77, 283–296.

    Article  Google Scholar 

  • Herschman, H.R. (1991).Ann. Rev. Biochem.,60, 281–319.

    Article  PubMed  CAS  Google Scholar 

  • Inouye, S., Guo, Y., de Paolo, L., Shimonaka, M., Ling, N. & Shimasaki, S. (1991).Endocrinology,129, 815–822.

    PubMed  CAS  Google Scholar 

  • Kingston, R.E. (1989). Guanidinium method for total RNA preparation.Current protocols in molecular biology. F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, K. Struhl (eds.). John Wiley and Sons, New York. Vol. I, pp. 4.2.1–4.2.5.

    Google Scholar 

  • Klein, R., Robertson, D.M., Shukovski, L., Findlay, J.K. & de Kretser, D.M. (1991).Endocrinology,128, 1048–1056.

    PubMed  CAS  Google Scholar 

  • Lamb, N.J.C., Fernandez, A., Tourkine, N., Jeanteur, P. & Blanchard, J.-M. (1990).Cell,61, 485–496.

    Article  PubMed  CAS  Google Scholar 

  • Li, R., Phillips, D.M. & Mather, J.P. (1995).Endocrinology,136, 849–856.

    Article  PubMed  CAS  Google Scholar 

  • Lindsell, C.D., Misra, V. & Murphy, B.D. (1994).J. Reprod. Fert.,100, 591–597.

    Article  CAS  Google Scholar 

  • Lindsell, C.E., Misra, V. & Murphy, B.D. (1993).Endocrinology,132, 1630–1636.

    Article  PubMed  CAS  Google Scholar 

  • Matzuk, M.M., Lu, N., Vogel, H., Sellheyer, K., Roop, D.R. & Bradley, A. (1995).Nature,374, 360–363.

    Article  PubMed  CAS  Google Scholar 

  • Michel, U., McMaster, J.W. & Findlay, J.K. (1992).J. Mol. Endrocrinol.,9, 147–156.

    CAS  Google Scholar 

  • Miyanaga, K., Shimasaki, S. (1993).Mol. Cell. Endocrinol.,92, 99–109.

    Article  PubMed  CAS  Google Scholar 

  • Murphy, B.D. (1994).Biol. Reprod.,50 (Suppl. 1), 133 (Abs 316).

    Google Scholar 

  • Nakamura, T., Takio, K., Eto, Y., Shibai, H., Titani, K. & Sugino, H. (1990).Science,247, 836–838.

    Article  PubMed  CAS  Google Scholar 

  • Nakatani, A., Shimasaki, S., de Paolo, L.V., Erickson, G.F. & Ling, N. (1991).Endocrinology,129, 603–611.

    PubMed  CAS  Google Scholar 

  • Richards, J.S. (1994).Endocrine Rev.,15, 725–751.

    Article  CAS  Google Scholar 

  • Robertson, D.M., Klein, R., de Vos, F.L., McLachlan, R.I., Wettenhall, R.E., Hearn, M.T.W., Burger, H.G. & de Kretser, D.M. (1987).Biochem. Biophys. Res. Commun.,149, 744–749.

    Article  PubMed  CAS  Google Scholar 

  • Shi, L.-Y., Zhang, Z.-W. & Li, Wei-Xiong. (1994).Endocrinology,134, 2431–2437.

    Article  PubMed  CAS  Google Scholar 

  • Shimasaki, S., Koga, M., Esch, F., Cooksey, K., Mercado, M., Koba, A., Ueno, N., Ying, S.-Y., Ling, N. & Guillemin, R. (1988a).Proc. Natl. Acad. Sci. USA,85, 4218–4222.

    Article  PubMed  CAS  Google Scholar 

  • Shimasaki, S., Koga, M., Esch, F., Mercado, M., Cooksey, K., Koba, A. & Ling, N. (1988b).Biochem. Biophys. Res. Commun.,152, 717–723.

    Article  PubMed  CAS  Google Scholar 

  • Shimasaki, S., Koga, M., Buscaglia, M.L., Simmons, D.M., Bicsak, T.A. & Ling, N. (1989).Mol. Endocrinol.,3, 651–659.

    PubMed  CAS  Google Scholar 

  • Shiozaki, M., Sakai, R., Tabuchi, M., Nakamura, T., Sugino, K., Sugino, H. & Eto, Y. (1992).Proc. Nat. Acad. Sci. USA,89, 1553–1556.

    Article  PubMed  CAS  Google Scholar 

  • Sugino, K., Kurosawa, N., Nakamura, T., Takio, K., Shimasaki, S., Ling, N., Tinati, K. & Sugino, H. (1993).J. Biol. Chem.,268, 15579–15587.

    PubMed  CAS  Google Scholar 

  • Themmen, A.P.N., Blok, L.J., Post, M., Baacerds, W.M., Hoogertrugge, J.W., Parmentier, M., Vascart, G. & Groitegoed, J.A. (1991).Mol. Cell Endo.,78, 1207–1213.

    Article  Google Scholar 

  • Tuuri, T., Eramaa, M., Hilden, K., Ritvos, O. (1994).Endocrinology,135, 2196–2203.

    Article  PubMed  CAS  Google Scholar 

  • Ueno, N., Ling, N., Ying, S.-Y., Esch, F., Shimasaki, S. & Guillemin, R. (1987).Proc. Natl. Acad. Sci. USA,84, 8282–8286.

    Article  PubMed  CAS  Google Scholar 

  • Woodruff, T.K., Lyon, R.J., Hanson, S.E., Rice, G.C. & Mather, J.P. (1990).Endocrinology,127, 3196.

    PubMed  CAS  Google Scholar 

  • Xiao, S., Findlay, J.K. & Robertson, D.M. (1990).Mol. Cell. Endocrinol.,69, 1–8.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Obstetrics and Gynecology, UCLA School of Medicine, Los Angeles, CA, USA

    C. E. Lindsell

  2. Department of Biological Chemistry, UCLA School of Medicine, Los Angeles, CA, USA

    C. E. Lindsell

  3. Department of Veterinary Microbiology, University of Saskatchewan, S7N OW0, Saskatoon, Saskatchewan

    V. Misra

  4. Centre de recherche en reproduction animale, Université de Montréal, C.P. 5000, J2S 7C6, St Hyacinthe, PQ, Canada

    B. D. Murphy

Authors
  1. C. E. Lindsell
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. V. Misra
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. B. D. Murphy
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lindsell, C.E., Misra, V. & Murphy, B.D. Follistatin has characteristics of a primary response gene in porcine granulosa cells. Endocr 3, 609–614 (1995). https://doi.org/10.1007/BF02953027

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02953027

Keywords

Search

Navigation