Abstract
We have described the expression of the prolactin (PRL) gene and the occurrence of PRL-like immunoreactive proteins in the hypothalamic-neurophypophyseal system of the rat. Here, we investigated the nature of neurohypophyseal PRL-like antigens, by studying the biological activity of medium conditioned by incubated neurohypophyses in specific bioassays for PRL and for the 16 kDa N-terminal fragment of PRL (16K PRL). Neurohypophyseal conditioned medium (NHCM) obtained after incubating neurohyphyseal lobes (1 h at 37°C) was enriched with proteins of 14 kDa and 23 kDa, that crossreacted with PRL-and 16K PRL-directed antisera. The NHCM stimulated in a dose-dependent fashion the proliferation of Nb2-lymphoma PRL-dependent cells. This effect paralleled that of PRL and 16K PRL standards and was neutralized by different dilutions of both PRL-and 16K PRL-antisera. Also, the NHCM inhibited the proliferation of endothelial cells in culture, an antiangiogenic-effect exerted by 16K PRL. The antiangiogenic effect of the NHCM was parallel to that of 16K PRL standard and neutralized by 16K PRL antiserum in a dose-dependent fashion. These results indicate that NHCM contains proteins that share receptor activation properties as well as antigenic determinants with both PRL and 16K PRL.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Andries, M., Tilemans, D. & Denef, C. (1992).Biochem. J.,281, 393–400.
Baldocchi, R.A., Tan, L., King, D.S. & Nicoll, C. (1993).Endocrinology,133, 935–938.
Clapp, C. (1987).Endocrinology,121, 2055–2064.
Clapp, C., Sears, P.S., Russell, D.H., Richards, J., Levay-Young, B.K. & Nicoll, C.S. (1988).Endocrinology,122, 2892–2898.
Clapp, C. & Weiner, R.I. (1992).Endocrinology,130, 1380–1386.
Clapp, C., Martial, J.A., Guzman, R.C., Rentier-Delrue, F. & Weiner, R.I. (1993).Endocrinology,133, 1292–1299.
Clapp, C., Torner, L., Gutiérrez-Ospina, G., Alcántara, E., López-Gómez, F.J., Nagano, M., Kelly, P.A., Mejía, S., Morales, M.A. & Martínez de la Escalera, G. (1994).Proc. Natl. Acad. Sci. USA,91, 10384–10388.
Cunningham, B.C. & Wells, J.A. (1989).Science,244, 1081–1085.
Cunningham, B.C. & Wells, J.A. (1991).Proc. Natl. Acad. Sci. USA,88, 3407–3411.
Cunningham, B.C., Ultsch, M., de Vos, A.M., Mulkerrin, M.G., Clauser, K.R. & Wells, J.A. (1991).Science,254, 821–825.
DeVito, W.J. (1988).Biochem. Biophys. Res. Commun.,150, 599–604.
Dutt, A., Kaplitt, M.G., Kow, L.M. & Pfaff, D.W. (1994):Neuroendocrinology,59, 413–419.
Emanuele, N.V., Metcalfe, L., Wallock, L., Tentler, J., Hagen, T.C., Beer, C.T., Martinson, D., Gout, P.W., Kirsteins, L. & Lawrence, A.M. (1986).Neuroendocrinology,44, 217–221.
Emanuele, N.V., Metcalfe, L., Wallock, L., Tentler, J., Hagen, T.C., Beer, C.T., Martinson, D., Gout, P.W., Kirsteins, L. & Lawrence, A.M. (1987).Brain Res.,421, 255–262.
Emanuele, N.V., Jurgens, J.K., Halloran, M.M., Tentler, J.J., Lawrence, A.M. & Kelley, M.R. (1992).Mol. Endocrinol.,6, 35–42.
Ferrara, N., Clapp, C. & Weiner, R.I. (1991).Endocrinology,129, 896–900.
Ferrara, N., Houck, K., Jakeman, L. & Leung, D.W. (1992).Endocr. Rev.,13, 18–32.
Fuh, G., Colosi, P., Wood, W.I. & Wells, J.A. (1993).J. Biol. Chem.,268, 5376–5381.
Harlan, R.E., Shivers, B.D., Fox, S.R., Kaplove, K.A., Schachters, B.S. & Pfaff, D.W. (1989).Neuroendocrinology,49, 7–22.
Harlan, R.E. & Scammell, J.G. (1991).J. Histochem. Cytochem.,39, 221–224.
Hooper, K.P., Padmanabhan, R. & Ebner, K.E. (1993).J. Biol. Chem.,268, 22347–22352.
Jackson, D., Volpert, O.V., Bouck, N. & Linzer, D.I.H. (1994).Science,266, 1581–1584.
Paut-Pagano, L., Roky, R., Valatx, J-L., Kitahama, K. & Jouvet, M. (1993).Neuroendocrinology,58, 682–695.
Rui, H., Lebrun, J-J., Kirken, R.A., Kelly, P.A. & Farrar, W.L. (1994).Endocrinology,135, 1299–1306.
Scammell, J.G., Wear, L.B. & Von Haven, R. (1990).Mol. Cell. Endocrinol.,71, 125–131.
Siaud, P., Manzoni, O., Balmefrezol, M., Barbanel, G., Assenmacher, I. & Alonso, G. (1980).Cell Tissue Res.,255, 107–115.
Sinha, Y.N. (1992).Trends Endocrinol. Metab.,3, 100–106.
Tanaka, T., Shiu, R.P.C., Gout, P.W., Beer, C.T., Noble, R.L. & Friesen, H.G. (1980).J. Clin. Endocrinol. Metab.,51, 1058–1063.
Torner, L., López, F., Mejía, S., Uribe, E., Noris, G., Quintanar, A., Martínez de la Escalera, G. & Clapp, C. (1994).76th Annual Meeting of the Endocrine Society, Anaheim: CA. p 249 (abstract).
Wilson III, D.M., Emanuele, N.V., Jurgens, J.K. & Kelley, M.R. (1992).Endocrinology,131, 2488–2490.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
López-Gómez, F.J., Torner, L., Mejía, S. et al. Immunoreactive prolactins of the neurohypophyseal system display actions characteristic of prolactin and 16K prolactin. Endocr 3, 573–578 (1995). https://doi.org/10.1007/BF02953021
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02953021