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Expression and localization of messenger RNA for tumor necrosis factor receptor (TNF-R) I and TNF-RII in pregnant mouse uterus and placenta

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Abstract

The temporal and cell-specific localization of tumor necrosis factor (TNF) receptor mRNAs in the uterus and placenta during pregnancy in the mouse was investigated. Messenger RNA for TNF and the TNF receptors (TNF-RI, p55/p60 and TNF-RII, p75/p80) was assessed by northern blot andin situ hybridization. TNF, TNF-RI and TNF-RII specific transcripts were present on days 7 through 18 of pregnancy. Relative concentrations of TNF mRNA decreased from days 7 to 18 with levels being higher in the uterus than the placenta. In contrast TNF-RI mRNA levels were constant throughout gestation and no differences were seen between steady state levels in the uterus and placenta. Two transcripts for TNF-RII (3.6 and 4.5 kb) were identified in all tissues. Steady state levels of TNF-RII mRNA increased throughout gestation and levels were higher in the placenta than in the uterus. On day 9 of gestation, TNF-RI and TNF-RII mRNAs were localized to undecidualized endometrium, mesometrial decidual cells, and the developing placenta. In addition, muscle cells contained TNF-RI but not TNF-RII mRNA. By day 15 of gestation, TNF-RI and TNF-RII transcripts were primarily localized to the uterine epithelium and trophoblast giant cells and spongiotrophoblast cells in the placenta. The results of these studies reveal the uterine and placental cell-specific expression of TNF receptor mRNAs during pregnancy in the mouse and provide insight into the cellular targets of TNF action.

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Author notes

  1. Nihay Laham

    Present address: Department of Perinatal Medicine, Royal Women’s Hospital, Carlton, Victoria, Australia

  2. Hendrick Kröning

    Present address: Abtl. Hematologie/Onkologie, Universitatsklininkum, Zentrum fur Innere Medizin, Magdeburg, Germany

Authors and Affiliations

  1. Department of Anatomy and Cell Biology, University of Kansas Medical Center, 66160-7400, Kansas City, Kansas, USA

    Katherine F. Roby, Nihay Laham, Hendrick Kröning & Joan S. Hunt

  2. Department of Physiology and Obstetrics and Gynecology, University of Kansas Medical Center, 66160-7400, Kansas City, Kansas, USA

    Paul F. Terranova

  3. Department of Pathology and Oncology, University of Kansas Medical Center, 66160-7400, Kansas City, Kansas, USA

    Joan S. Hunt

Authors
  1. Katherine F. Roby
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  2. Nihay Laham
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  3. Hendrick Kröning
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  4. Paul F. Terranova
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  5. Joan S. Hunt
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Roby, K.F., Laham, N., Kröning, H. et al. Expression and localization of messenger RNA for tumor necrosis factor receptor (TNF-R) I and TNF-RII in pregnant mouse uterus and placenta. Endocr 3, 557–562 (1995). https://doi.org/10.1007/BF02953019

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  • DOI: https://doi.org/10.1007/BF02953019

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