Applied Biochemistry and Biotechnology

, Volume 36, Issue 1, pp 63–74

Fructose production

Coimmobilized amyloglucosidase, pullulanase, and glucose isomerase on BIOBONETM
  • Doris Y. Schafhagser
  • Kenneth B. Storey

DOI: 10.1007/BF02950775

Cite this article as:
Schafhagser, D.Y. & Storey, K.B. Appl Biochem Biotechnol (1992) 36: 63. doi:10.1007/BF02950775


Amyloglucosidase, pullulanase, and glucose isomerase were coimmobilized onto granular chicken bone (BIOBONETM). Enzyme ratios showing optimum glucose and fructose production (0.7:10:22.3 U amyloglucosidase: pullulanase: glucose isomerase) resulted in 14.4±1.9% of activity bound relative to an equal amount of free enzyme. The estimated specific activity for these enzymes decreased 4.6-fold with immobilization. ReactionpH strongly influenced the yield and ratio of glucose and fructose produced. Net hexose production from the immobilized system was optimal atpH 6.5 and 55°C with a fructose yield of about 20%.

Index Entries

Amyloglucosidase chicken bone pullulanase enzyme coimmobilization glucose isomerase high fructose syrup 

Copyright information

© Humana Press Inc. 1992

Authors and Affiliations

  • Doris Y. Schafhagser
    • 1
  • Kenneth B. Storey
    • 1
  1. 1.Institute of Biochemistry, Departments of Biology and ChemistryCarleton UniversityOttawaOntario Canada

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