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Biocatalytic preparation of chiral epichlorohydrins using recombinantPichia pastoris expressing epoxide hydrolase ofRhodotorula glutinis

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Abstract

The use of enantioselective hydrolysis for preparing chiral epichlorohydrins was investigated using recombinantPichia pastoris with the enantioselective epoxide hydrolase ofRhodotorula glutinis. The rate of the recombinant epoxide hydrolase-catalyzed enantioselective hydrolysis of racemic epichlorohydrins was enhanced by the addition of 5% (v/v) Tween 20. Enantiopure (R)-epichlorohydrins with an enantiopurity of 100%ee and a yield of 26% were obtained within 5 min from 50 mM racemates.

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Authors and Affiliations

  1. Department of Food Science and Technology, Kyungsung University, 608-736, Busan, Korea

    Hee Sook Kim & Eun Yeol Lee

  2. Department of Bioscience and Biotechnology, Silla University, 617-736, Busan, Korea

    Jae-Hwa Lee

  3. Department of Chemical and Biochemical Engineering, Pusan National University, 609-735, Busan, Korea

    Sunghoon Park

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  1. Hee Sook Kim
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  2. Jae-Hwa Lee
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  3. Sunghoon Park
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  4. Eun Yeol Lee
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Kim, H.S., Lee, JH., Park, S. et al. Biocatalytic preparation of chiral epichlorohydrins using recombinantPichia pastoris expressing epoxide hydrolase ofRhodotorula glutinis . Biotechnol Bioproc E 9, 62–64 (2004). https://doi.org/10.1007/BF02949324

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  • DOI: https://doi.org/10.1007/BF02949324

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