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Use of moving aeration membrane bioreactor for the efficient production of tissue type plasminogen activator in serum free medium

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Abstract

A moving aeration-membrane (MAM) bioreactor was employed for the production of 2 μg/mL of tissue type Plasminogen Activator (tPA) in serum free medium from normal human fibroblast cells. This system could maintain high cell density for long periods of steady state conditions in perfusion cultivation. Under normal operating conditions, shear stress was as low as 0.65 dynes/cm2 at the agitation speed of 80 rpm. Even though cell density gradually decreased with increasing agitation speed, tPA production increased linearly with increasing shear stress within a moderate range. This culture system allowed production of 2 μg tPA/mL while maintaining a high cell density of 1.0×107 viable cells/mL.

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Authors and Affiliations

  1. Division of Food and Biotechnology, Kangwon National University, 200-701, Chunchon, Korea

    Hyun Koo Kim, Moon Sun Ham, Jong Soo Hong, Jin Ha Lee, Kyung Yu Park & Hyeon Yong Lee

  2. Department of Medicine, Hanllym Medical School, 200-601, Chunchon, Korea

    Hyun Koo Kim, Moon Sun Ham, Jong Soo Hong, Jin Ha Lee, Kyung Yu Park & Hyeon Yong Lee

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  1. Hyun Koo Kim
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  2. Moon Sun Ham
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  3. Jong Soo Hong
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  4. Jin Ha Lee
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Correspondence to Hyeon Yong Lee.

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Kim, H.K., Ham, M.S., Hong, J.S. et al. Use of moving aeration membrane bioreactor for the efficient production of tissue type plasminogen activator in serum free medium. Biotechnol. Bioprocess Eng. 1, 32–35 (1996). https://doi.org/10.1007/BF02949141

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