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Effect of incorrectly estimated parameters on the control of specific growth rate inE. coli fed-batch fermentation

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Abstract

An exponential feeding strategy has been frequently used in fed-batch fermentation of recombinantE. coli. In this feeding scheme, growth yield and initial cell concentration, which can be erroneously determined, are needed to calculate the feed rate for controlling specific growth rate at the set point. The effect of the incorrect growth yield and initial cell concentration on the control of the specific growth rate was theoretically analyzed. Insignificance of the correctness of those parameters for the control of the specific growth rate was shown theoretically and experimentally.

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Author notes

  1. Tai Hyun Park

    Present address: Department of Chemical Engineering, Seoul National University, Seoul, Korea

Authors and Affiliations

  1. Department of Genetic Engineering, Sung Kyun Kwan University, Suwon, Korea

    Tai Hyun Park

  2. Lucky Biotech Research Institute, P.O. Box 10, Daejeon, Korea

    Sung Kwan Yoon

  3. Department of Chemical Engineering, Han Nam University, Daejeon, Korea

    Whan Koo Kang

Authors
  1. Tai Hyun Park
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  2. Sung Kwan Yoon
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  3. Whan Koo Kang
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Park, T.H., Yoon, S.K. & Kang, W.K. Effect of incorrectly estimated parameters on the control of specific growth rate inE. coli fed-batch fermentation. Biotechnol. Bioprocess Eng. 1, 22–25 (1996). https://doi.org/10.1007/BF02949139

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