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Effect ofin vitro digestion on fumonisin B1 in corn flakes

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Abstract

Low levels of fumonisins have been found frequently in corn based breakfast cereals and can occur bound to protein and other matrix components.In vitro digestion of two samples of corn flakes was carried out under "fed conditions." Fumonisins were measured as o-phthaldialdehyde/mercaptoethanol derivatives by LC-fluorescence. One sample of corn flakes (FN12) had high concentrations of fumonisin B1 (FB) (average 125 ng/g) and total bound FB1, (TB FB1) (average 92 ng/g) and the other (FN11) had a low level of free FB1 (average 29 ng/g) and no detectable TB FB1. After incubation of the samples with gastrointestinal tract solutions simulating saliva plus stomach and duodenal juices, chyme was analysed for FB1, hydrolyzed FB1 (HFB1) and partially hydrolyzed fumonisin B1 (PHFB1). The bioaccessibility (percentage of FB1 released from corn flakes into chyme) was 38-78% for incurred FB1 in FN12, 8-54% for incurred plus spiked FB1 in FN12, and 19-66% for incurred plus spiked FB1 in FN11. HFB1 and PHFB1 were not detected. If free FB1 was first extracted from sample FN12, no FB1 was detected in the chyme, indicating no contribution from TB FB1. Concentrations were corrected for method recovery of FB1 or, for bound FB1, partial method recovery of HFB1

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References

  1. Marasas WFO (2001) Discovery and occurrence of the fumonisins: a historical perspective. Environ Health Perspect 109 (suppl. 2): 239–243

    Article  PubMed  CAS  Google Scholar 

  2. Jackson L, Jablonski J (2004) Fumonisins. In: Nagan M, Olsen M (eds) Mycotoxins in Food. Detection and Control. Woodhead, Cambridge, 367–405

    Google Scholar 

  3. Soriano JM, Dragacci S (2004) Occurrence of fumonisins in foods. Food Res Int 37: 985–1000

    Article  CAS  Google Scholar 

  4. Rheeder JP, Marasas WFO, Thiel PF, Sydenham EW, Shephard GS, vanSchalkwyk DJ (1992)Fusarium moniliforme and fumonisins in corn in relation to human esophageal cancer in Transkei. Phytopathology 82: 353–357

    Article  Google Scholar 

  5. Chu FS, Li GY (1994) Simultaneous occurrence of fumonisin B1 and other mycotoxins in moldy corn collected from the People’s Republic of China in regions with high incidences of esophageal cancer. Appl Environ Microbiol 60: 847–852

    PubMed  CAS  Google Scholar 

  6. Sadler TW, Merrill AH, Stevens VL, Sullards MC, Wang E, Wang P (2002) Prevention of fumonisin B1-induced neural tube defects by folic acid. Teratology 66: 169–176

    Article  PubMed  CAS  Google Scholar 

  7. Marasas WF, Riley RT, Hendricks KA, Stevens VL, Sadler TW, Gelineau-van Waes J, Missmer SA, Cabrera J, Torres O, Gelderblom WCA, Allegood J, Martinez C, Maddox J, Miller JD, Starr L, Sullards MC, Roman AV, Voss KA, Wang E, Merrill AHJr (2004) Fumonisins disrupt sphingolipid metabolism, folate transport, and neural tube development in embryo culture and in vivo: a potential risk factor for human neural tube defects among populations consuming fumonisin-contaminated maize. J Nutr 134: 711–716

    PubMed  CAS  Google Scholar 

  8. Missmer SA, Suarez L, Felkner M, Wang E, Merrill AHJr, Rothman KJ, Hendricks KA (2006) Exposure to fumonisins and the occurrence of neural tube defects along the Texas-Mexico border. Environ Health Perspect 114: 237–241

    Article  PubMed  Google Scholar 

  9. Humpf H-U, Voss KA (2004) Effects of thermal food processing on the chemical structure and toxicity of fumonisin mycotoxins. Mol Nutr Food Res 48: 255–269

    Article  PubMed  CAS  Google Scholar 

  10. Castelo MM, Katta SK, Sumner SS, Hanna MA, Bullerman LB (1998) Extrusion cooking reduces recoverability of fumonisin B1 from extruded corn grits. J Food Sci 63: 696–698

    CAS  Google Scholar 

  11. Abd Alla El-Sayed AM, Aly Soher E, Sahab AF (2003) Occurrence of certain mycotoxins in corn and corn-based products and the thermostability of fumonisin B1 during processing. Nahrung 47: 222–225

    Article  CAS  Google Scholar 

  12. Jackson LS, Katta SK, Fingerhut DD, De Vries JW, Bullerman LB (1997) Effects of baking and frying on the fumonisin B1 content of cornbased foods. J Agric Food Chem 45: 4800–4805

    Article  CAS  Google Scholar 

  13. Meister U, Springer M (2004) Mycotoxins in cereal products-occurrence and changes during processing. J Appl Bot Food Qual 78: 168–173

    CAS  Google Scholar 

  14. Katta SK, Jackson LS, Sumner SS, Hanna MA, Bullerman LB (1999) Effect of temperature and screw speed on stability of fumonisin B1 in extrusion-cooked corn grits. Cereal Chem 76: 16–20

    Article  CAS  Google Scholar 

  15. Dombrink-Kurtzman MA, Dvorak TJ, Barron ME, Rooney LW (2000) Effect of nixtamalization (alkaline cooking) on fumonisin-contaminated corn for production of masa and tortillas. J Agric Food Chem 48: 5781–5786

    Article  PubMed  CAS  Google Scholar 

  16. Cassaniga D, Basilico JC, Gonzalez RJ, Torres RL, de Greef DM (2001) Mycotoxins inactivation by extrusion cooking of corn flour. Lett Appl Microbiol 33: 144–147

    Article  Google Scholar 

  17. Saunders DS, Meredith FI, Voss KA (2001) Control of fumonisin: Effects of processing. Environ Health Perspect 109, Suppl 2: 333–336

    Article  PubMed  CAS  Google Scholar 

  18. Castells M, Marin S, Sanchis V, Ramos AJ (2005) Fate of mycotoxins in cereals during extrusion cooking: A review. Food Addit Contam 22: 150–157

    Article  PubMed  CAS  Google Scholar 

  19. De Girolamo A, Solfrizzo M, Visconti A (2001) Effect of processing on fumonisin concentration in corn flakes. J Food Protect 64: 701–705

    Google Scholar 

  20. Jackson LS, Hlywka JJ, Senthil KR, Bullerman LB, Musser SM (1996) Effects of time, temperature, and pH on the stability of fumonisin B1 in an aqueous model system. J Agric Food Chem 44: 906–912

    Article  CAS  Google Scholar 

  21. Shier WT, Abbas HK, Badria FA (1997) Structure-activity relationships of the corn fungal toxin fumonisin B1: Implications for food safety. J Nat Toxins 6: 225–242

    CAS  Google Scholar 

  22. Kim E-K, Scott PM, Lau BP-Y (2003) Hidden fumonisin in corn flakes. Food Addit Contam 20: 161–169

    Article  PubMed  CAS  Google Scholar 

  23. Park JW, Scott PM, Lau BP-Y, Lewis DA (2004) Analysis of heat-processed corn foods for fumonisins and bound fumonisins. Food Addit Contam 21: 1168–1178

    Article  PubMed  CAS  Google Scholar 

  24. Meister U (2001) Investigations on the change of fumonisin content of maize during hydrothermal treatment of maize. Analysis by means of HPLC methods and ELISA. Eur Food Res Technol 213: 187–193

    Article  CAS  Google Scholar 

  25. Seefelder W, Humpf H-U (2001) Formation of fumonisin artefacts in thermal treated food. Mycotoxin Research 17A: 116–119

    Article  Google Scholar 

  26. Seefelder W, Knecht A, Humpf, H-U (2003) Bound fumonisin B1: Analysis of fumonisin-B1 glyco and amino acid conjugates by liquid chromatography-electrospray ionization-tandem mass spectrometry. J Agric Food Chem 51: 5567–5573

    Article  PubMed  CAS  Google Scholar 

  27. Marasas WFO, Miller JD, Riley RT, Visconti A (2000) Fumonisins. Environmental Health Criteria 219. World Health Organization, Geneva

    Google Scholar 

  28. Shephard GS, Thiel PG, Sydenham EW, Savard ME (1995) Fate of a single dose of14C-labelled fumonisin B) in vervet monkeys. Nat Toxins 3: 145–150

    Article  PubMed  CAS  Google Scholar 

  29. Shephard GS, Thiel PG, Sydenham EW, Alberts JF, Cawood ME (1994) Distribution and excretion of a single dose of the mycotoxin fumonisin B1 in a non-human primate. Toxicon 32: 735–741

    Article  PubMed  CAS  Google Scholar 

  30. Norred WP, Plattner RD, Chamberlain WJ (1993) Distribution and excretion of [14C] fumonisin B1 in male Sprague-Dawley rats. Nat Toxins 1: 341–346

    Article  PubMed  CAS  Google Scholar 

  31. Shephard GS, Thiel PG, Sydenham EW, Alberts JF, Gelderblom WCA (1992) Fate of a single dose of the14C-labelled mycotoxin, fumonisin B1, in rats. Toxicon 30: 768–770.

    Article  PubMed  CAS  Google Scholar 

  32. Hopmans EC, Hauck CC, Hendrich S, Murphy PA (1997) Excretion of fumonisin B1 hydrolyzed fumonisin B1, and the fumonisin B1-fructose adduct in rats. J Agric Food Chem 45: 2618–2625

    Article  CAS  Google Scholar 

  33. Shier WT (2000) The fumonisin paradox: a review of research on oral bioavailability of fumonisin B1, a mycotoxin produced byFusarium moniliforme. J Toxicol Toxin Rev 19: 161–187

    CAS  Google Scholar 

  34. Oomen AG, Rompelberg JM, Bruil MA, Dobbe CJG, Pereboom DPKH, Sips AJAM (2003) Development of anin vitro digestion model for estimating the bioaccessibility of soil contaminants. Arch Environ Contam 44: 281–287

    Article  CAS  Google Scholar 

  35. Versantvoort CHM, Oomen AG, Van de Kamp E, Rompelberg CJM, Sips AJAM (2005) Applicability of an in vitro digestion model in assessing the bioaccessibility of mycotoxins from food. Food Chem Toxicol 43: 31–40

    Article  PubMed  CAS  Google Scholar 

  36. Rice LG, Ross PF (1994) Methods for detection and quantitation of fumonisins in corn, cereal products and animal excreta. J Food Protect 57: 536–540

    Google Scholar 

  37. Thakur RA, Smith JS (1996). Determination of fumonisins B1 and B2 and their major hydrolysis products in corn, feed and meat, using HPLC. J Agric Food Chem 44: 1047–1052

    Article  CAS  Google Scholar 

  38. Xie W, Mirocha CJ, Chen J (1997) Detection of two naturally occurring structural isomers of partially hydrolyzed fumonisin B1 in corn by on-line capillary liquid chromatography-fast atom bombardment mass spectrometry. J Agric Food Chem 45: 1251–1255

    Article  CAS  Google Scholar 

  39. Sydenham EW, Thiel GP, Shephard GS, Koch KR, Hutton T (1995) Preparation and isolation of partially hydrolyzed moiety of fumonisin B1. J Agric Food Chem 43: 2400–2405

    Article  CAS  Google Scholar 

  40. Scott PM, Lawrence GA (1996) Determination of hydrolyzed fumonisin B1 in alkali-processed corn foods. Food Addit Contam 13: 823–832

    PubMed  CAS  Google Scholar 

  41. Shephard GS, Thiel PG, Sydenham EW, Vleggaar R, Alberts JF (1994) Determination of the mycotoxin fumonisin B1 and identification of its partially hydrolysed metabolites in the faeces of non-human primates. Food Chem Toxicol 32: 23–29

    Article  PubMed  CAS  Google Scholar 

  42. Täubel M, Moll, WD, Vekiru E, Frank A, Loibner AP, Braun R, Schatzmayr G (2006) Biological detoxification of fumonisins by new bacterial isolates. Mycoglobe International Conference, Monopoli (Bari), Italy, 26–29 September, 2006, poster 15

  43. Karlovsky P (1999) Biological detoxification of fungal toxins and its use in plant breeding, feed and food production. Nat Toxins 7: 1–23

    Article  PubMed  CAS  Google Scholar 

  44. Benedetti R, Nazzi F, Locci R, Firrao G (2006) Degradation of fumonisin B1 by a bacterial strain isolated from soil. Biodegradation 17: 31–38

    Article  PubMed  CAS  Google Scholar 

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  1. E. L. Motta

    Present address: Departamento de Quimica, Facultad de Ciencias Exactas y Naturales, Universidad Mar del Plata, (7600) Funes 3350, Mar del Plata, Argentina

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  1. Bureau of Chemical Safety, Health Canada, Address Locator 2203D, K1A0K9, Ottawa, Ontario, Canada

    E. L. Motta & P. M. Scott

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Motta, E.L., Scott, P.M. Effect ofin vitro digestion on fumonisin B1 in corn flakes. Mycotox Res 23, 166–172 (2007). https://doi.org/10.1007/BF02946043

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