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Development of a proteomic approach to monitor protein synthesis in mycotoxin producing moulds

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Abstract

In general, proteome studies compare different states of metabolism to investigate external or internal influences on protein expression. In the context of mycotoxin production the method could open another view on this complex and could be helpful to gain knowledge about proteins which are involved in metabolism (enzymes, transporters). In this short technical report, we describe a new protocol suitable for protein preparation for whole proteome analysis ofFusarium graminearum. Cell lysis was performed by grinding the mycelium with liquid nitrogen. Proteins were extracted with TCA/acetone and then cleaned; the isolated proteins were separated in a 2D-gel electrophoresis system (BioRad) using different pH gradients. The protocol established seems also generally applicable for other mycotoxin producing fungi.

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Authors and Affiliations

  1. Institut für Ernährungs-und Lebensmittelwissenschaften, Lebensmittel-Mikrobiologie und Hygiene, Rheinische Friedrich-Wilhelms-Universität Bonn, Meckenheimer Allee 168, 53115, Bonn, Germany

    J. Milles & J. Krämer

  2. Mikrobiologie und Lebensmittelhygiene, Fachbereich Oecotrophologie, Hochschule Niederrhein, Rheydter Straße 277, 41065, Mönchengladbach, Germany

    A. Prange

  3. Center for Advanced Microstructures & Devices (CAMD), Louisiana State University, 6980 Jefferson Hwy., 70806, Baton Rouge, LA, USA

    A. Prange

Authors
  1. J. Milles
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  2. J. Krämer
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  3. A. Prange
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Correspondence to A. Prange.

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Financial support: Cusanuswerk (doctoral scholarship)

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Milles, J., Krämer, J. & Prange, A. Development of a proteomic approach to monitor protein synthesis in mycotoxin producing moulds. Mycotox Res 23, 161–165 (2007). https://doi.org/10.1007/BF02946042

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  • DOI: https://doi.org/10.1007/BF02946042

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