Abstract
Callus induction, plantlet regeneration and their field establishment were achieved in the genusErianthus which is closely related toSaccharum. The species utilised wasE. elephantinus with the lowest chromosome number of the genus (2n = 20). The donor clone SES 305 is cytologically stable. The 64 somaclones which attained maturity and were studied did not show much morphological variation. However, many somaclones showed consistantly low pollen fertility over years. Although chromosome numerical variation was rare structural aberrations such as occurrence of a fragment, chromosome fusion, ring chromosomes, laggards, bridges etc. were noticed during root tip mitosis and meiosis. The data did not indicate any relationship between pollen fertility and meiotic abnormalities.
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References
Ahloowalia, B.S. (1976). Chromosomal changes in parasexually produced rye grass, pp. 115–117.In: K., Jones, PE, Brandham (eds). Current Chromosome Research, Amsterdam, Elsevier.
Alexander, M.P. (1969). Differential staining of aborted and non aborted pollen.Stain Tech.,44: 117–122.
Cheng, T.Y. and Smith, H.H. (1975). Organogenesis from callus culture ofHordeum vulgare.Planta,123: 307–310.
Darlington, C.D and Cour, F. La. (1976). Handling of chromosomes. George allen and Un Win Ltd. Six Edition, pp. 131–132.
Gamorg, O.L., Shyluk, J.P., Brar, D.S. and Constable, F. (1977). Morphogenesis and plant regeneration from callus of immature embryos of Sorghum.Plant Sci. Lett,10: 67–74.
Gosch-Wackerle. G., Avivi, L. and Galun, E. (1979). Induction, culture and differentiation of callus from immature ra-chises, seeds and embryo ofTriticum.Z. pflanzenphysiol,91: 267–278.
Hanna, W.W., Lu., C. and Vasil, I.K. (1984). Uniformity of plants regenerated from somatic embryos ofPanicum maximum Jacq (Guinea grass).Theor. Appl. Genet,67: 155–159.
Hayadu, Z. and Vasil, I.K. (1981). Somatic embryogenesis and plant regeneration from leaf tissues and anthers ofPennisetum purpureum Schum.Theor. Appl. Genet.,59: 29–273.
Heinz, D.J., Krishnamurthy, M., Nickeil, L.G. and Maretzki, A. (1977). Cell tissue and organ culture in sugarcane improvement, pp 7–17.In: Applied and fundamental aspects of plant cell, tissue and organ culture Reinert and YPS, Bajaj (eds) Berlin, Springier.
Jagathesan, D. and Sreenivasan, T.V. (1971). Cytogenetical studies inErianthus II: Occurence of chromosome knobs.Caryologia,24 : 27–31.
Kasperbauer, M.J., Buekner, R.C. and Bush, L.P. (1979). Tissue culture of annual grass tall fescue F1 hybrids. Callus establishment and plant regeneration.Crop Science,19: 457–460.
Larikin, P.J., Rayan, S.A., Bretell, R.I.S. and Scowcroft, W.R. (1984). Heritable somaclonal variation in wheat.Theor. Appl. Genet.,67: 443–455.
Lu, C. and Vasil, I.K. (1981). Somatic embryogenesis and plant regeneration from leaf tissues ofPanicum maximum Jacq.Theor. Appl. Genet,59: 275–280.
Lu, C. and Vasil, I.K. (1982). Somatic embryogenesis and plant regeneration in tissue culture ofPanicum maximum Jacq.Am. J. Bot.,69: 77–81.
Lu, C., Vasil, I.K. and Ozias-Akins, P. (1982). Somatic embryogenesis inZea mays L.Theor. Appl. Genet,62: 109–112.
Me Coy, T.J. and Philip, R.L. (1982). Chromosomal stability in maize (Zea mays L) tissue cultures and sectoring among regenerated plants.Can J. Genet Cytol,24: 259–566.
Me Coy, T.J., Philip, R.L. and Rines, H.W. (1982). Cytoge-netic analysis of plants regenerated from Oat (Avena sativa) tissue culture, high frequency of partial chromosome loss.Can J. Genet. Cytol,24: 37–50.
Murata, M. and Orton, T.J. (1984). Chromosome fusions in cultured cells of celery.Can J. Genet. Cytol.,26: 395–400.
Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bioassay with tobacco tissue culture.Physiol. Plant,15: 473–497.
Nishi, T. and Mitsuoka, S. (1969). Occurrence of various ploidy plants from anther and ovary culture of rice plant,Jap J. Genet,44: 341–346.
Oono, K. (1978). Test tube breeding of rice by tissue culture.Trop. Agric. Res. Ser,11: 109–123.
Orton, T.J. (1980). Chromosomal variability in tissue cultures and regenerated plants ofHordeum.Theor. Appl. Genet,56: 101–112.
Ostergren, G. and Heneen, W.K. (1962). A squash technique for chromosome morphological studies.Hereditas,33: 261–296.
Ozias-Akins, P. and Vasil, I.K. (1982). Plant regeneration from cultured immature embryos and inflorescence ofTriticum aestivum (Wheat) Evidence for somatic embryogenesis. Protoplasma,110: 95–105.
Shimada T. and Yamada, A. (1979). Wheat plants regenerated from embryo cell cultures,Jap. J. Genet,54: 379–385.
Singh, R.J. (1986). Chromosome variation in immature embryo derived calluses of Barley (Hordeum vulgare L).Theor. Appl. Cenet,72: 710–716.
Vasil, V. and Vasil, I.K. (1981 a). Somatic embryogenesis and plant regeneration from tissue cultures ofPennisetum americanum andP. purpureum hybrid.Am. J. Bot,68: 864–872.
Vasil, V. and Vasil, I.K. (1981 b). Somatic embryogenesis and plant regeneration from suspension cultures of pearl millet (Pennisetum americanum).Ann. Bot,47: 669–678.
Vasil, I.K., Vasil, V., Lu., C.P., Ozias-Akins, Hayadu., Z. and Wang, D. (1982). Somatic embryogenesis in cereals and grasses, pp. 3–21.In: (eds) Variability in plants regenerated from tissue Culture E. Earle, Y. Demarly, New York, Praeger.
Wang, D and Vasil, I.K. (1982). Somatic embryogenesis and plant regeneration from inflorescence segments ofPennisetum purpureum (Napier or elephant grass).Plant Sci. Lett,25: 147–154.
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Jalaja, N.C., Sreenivasan, T.V. In vitro Regeneration and Cytological Studies on Somaclones ofErianthus elephantinus Hook. F.. Sugar Tech 1, 132–138 (1999). https://doi.org/10.1007/BF02945187
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DOI: https://doi.org/10.1007/BF02945187