Abstract
Selected formulations of the entomopathogenic fungusBeauveria brongniartii were evaluated for conidial viability by media plating method and bioassay against eggs and larvae of its target hostHolotrichia serrata F. (Coleoptera: Scarabaeidae). When inoculated on rose Bengal agar medium, talc formulation (9 months) produced the highest colony forming units (CFUs) among all the formulations giving formulation strength of 2.4 - 4.2 x 106 CFUs/g in pour-plate method and 1.2 - 6.0 x 106 CFUs/g in spread-plate method. Lignite (4 months) produced colonies by both methods giving variable formulation strength at different dilutions. The detected strength (CFUs/g) was far lower than the label strength (spores/g) for these formulations. Formulations with vermicast, lignite (both 16 months) and dry yeast (7 months) as carrier materials did not produce colonies. In bioassay tests withH. serrata eggs, formulations produced lower infection rates (0-12%) than pure conidial suspension (28%). In third instar grubs too, formulations produced lower mortality rates (0-40%) than pure conidial suspensions (100%). The study indicated suitability of talc and lignite as carrier material and a shelf life of less then 12 months.
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Srikanth, J., Santhalakshmi, G. & Tamizharasi, V. Viability and virulence of selectedbeauveria brongniartii formulations againstHolotrichia serrata . Sugar Tech 8, 152–154 (2006). https://doi.org/10.1007/BF02943651
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DOI: https://doi.org/10.1007/BF02943651