Abstract
In order to analyze liquid growth media for fumonisin B1, B2 and hydrolyzed fumonisin B1 (HFB1) after incubation with microorganisms for degradation studies, a liquid chromatography/electrospray ionisation mass spectrometry (LC/ESI-MS) method was developed. The use of phytosphingosine hydrochloride (PSH) and T-2-toxin-d3 (T2d3) as internal standard has been tested. The detection limit established with PSH as an internal standard was about 20 ng/mL for FB1 and FB2 and about 50 ng/mL for HFB1. The developed method allows the rapid, simultaneous and quantitative determination of these analytes in microbial culture media without any cleanup.
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Vekiru, E., Fuchs, E., Schatzmayr, G. et al. Determination of fumonisins and hydrolyzed fumonisin B1 in microbial culture media by LC/ESI-MS. Mycotox Res 19, 198–202 (2003). https://doi.org/10.1007/BF02942965
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DOI: https://doi.org/10.1007/BF02942965