Abstract
To enhance the productivity and activity of nitrile hydratase inRhodococcus rhodochrous M33, a glucose-limited fed-batch culture was performed. In a fed-batch culture where the glucose was controlled at a limited level and cobalt was supplemented during the fermentation period, the cell mass and total activity of nitrile hydratase both increased 3.3-fold compared to that in the batch fermentation. The productivity of nitrile hydratase also increased 1.9-fold compared to that in the batch fermentation. The specific activity of nitrile hydratase in the whole cell preparation when using a fed-batch culture was 120 units/mg-DCW, which was similar to that in the batch culture.
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Kim, BY., Kim, JC., Lee, HH. et al. Fed-batch fermentation for production of nitrile hydratase byRhodococcus rhodochrous M33. Biotechnol. Bioprocess Eng. 6, 11–17 (2001). https://doi.org/10.1007/BF02942244
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DOI: https://doi.org/10.1007/BF02942244