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Characterization of the α-mannosidase gene family in filamentous fungi: N-glycan remodelling for the development of eukaryotic expression systems

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Abstract

Although filamentous fungi are used extensively for protein expression, their use for the production of heterologous glycoproteins is constrained by the types of N-glycan structures produced by filamentous fungi as compared to those naturally found on the glycoproteins. Attempts are underway to engineer the N-glycan synthetic pathways in filamentous fungi in order to produce fungal expression strains which can produce heterologous glycoproteins carrying specific N-glycan structures. To fully realize this goal, a detailed understanding of the genetic components of this pathway in filamentous fungi is required. In this review, we discuss the characterization of the α-mannosidase gene family in filamentous fungi and its implications for the elucidation of the N-glycan synthetic pathway.

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  1. Department of Biology, University of Victoria, P.O. Box 3020 STN CSC, V8W 3N5, Victoria, British Columbia, Canada

    C. Joshua Eades & William E. Hintz

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  1. C. Joshua Eades
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Correspondence to William E. Hintz.

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Eades, C.J., Hintz, W.E. Characterization of the α-mannosidase gene family in filamentous fungi: N-glycan remodelling for the development of eukaryotic expression systems. Biotechnol. Bioprocess Eng. 5, 227–233 (2000). https://doi.org/10.1007/BF02942178

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