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Modeling cassava starch saccharification with amyloglucosidase

  • Session 3 Bioprocessing Research
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Abstract

A solution of α-amylase liquefied cassava starch, 30% (w/v), was saccharified with amyloglucosidase at 45°C, pH 4.5, in a batch reactor in the presence and absence of added glucose. Reactor conversion results were modeled with a multisubstrate model that considers intermediate dextrins of starch hydrolysis, reversibility of some reactions, substrate and product inhibition, and competition among dextrins and isomaltose formation. Kinetic parameters were obtained from initial velocity saccharification tests at different starch concentrations and from the literature. The model can represent well the saccharification of cassava starch even in the presence of a great excess of glucose (100 g/L), added to test its capability.

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Abbreviations

CA :

liquefied starch concentration, g/L

CAo :

initial starch concentration, 300 g/L

Cg :

glucose concentration, g/L

Cga :

concentration of added glucose, g/L

Cgi :

glucose concentration at the start of saccharification, g/L

E:

enzyme concentration, mL of enzyme stock solution/L of substrate solution

f:

ratio of molecular weights for the anhydroglucose unit in starch and glucose, f = 162/180 = 0.9

G:

glucose molar concentration, mol/L

G2 :

maltose molar concentration, mol/L

G3 :

maltotriose molar concentration, mol/L

G4 :

susceptible oligosaccharides molar concentration, mol/L

G6 :

resistant oligosaccharides molar concentration, mol/L

GI :

isomaltose molar concentration, mol/L

Geq, G2eq, G3eq, Gleq :

quilibrium molar concentration for glucose, maltose, maltotriose, and isomaltose, respectively, mol/L

kcat :

reaction rate constant related to product formation, ol/(h·mL of enzyme)

Keq2, Keq3, KeqI :

equilibrium constants for maltose (mol/L), maltotriose (mol/L), and isomaltose (L/mol), respectively

Ki :

product (glucose) inhibition constant, mol/L

Km2, Km3, Km4, Km6 :

Michaelis-Menten constants for maltose, maltotriose, susceptible oligosaccharides, and resistant oligosaccharides, respectively, mol /L

KS :

substrate inhibition constant, mol/L

MG :

molecular weight of glucose, 180 g/gmol

n:

average degree of polymerization, dimensionless

r2, r3, r4, r6, r1 :

rate of reaction for maltose, maltotriose, susceptible oligosaccharides, resistant oligosaccharides, and isomaltose, respectively, mol/(L·h)

t:

reaction time, h

V:

initial rate of glucose production, g/(L·h)

VIM :

second-order rate constant for isomaltose, L2/(mol·h·mL of enzyme)

Vm2, Vm3, Vm4, Vm6 :

maximum velocity constants associated with the reaction rate of maltose, maltotriose, susceptible oligosaccharides, and resistant oligosaccharides, respectively, mol/(h·mL of enzyme)

XA :

conversion of liquefied starch to glucose, %

α:

parameter in Eq. (22), a = (CAo/kcat) (1-Km/K+CAo/KS), mL of enzyme·h/L

β:

parameter in Eq. (22), β = (Km/kcat) (1 + Cgi/Ki + CAo/Ki), mL of enzyme·h/L

γ:

parameter in Eq. (22), γ = C 2Ao /(2K S kcat), ml of enzymeh/L

τb :

normalized reaction time, τb = E·t, mL of enzyme·h/L

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Authors and Affiliations

  1. Chemical Engineering Department, State University of Maringá, 87020-900, Maringá, PR, Brazil

    Gisella Maria Zanin & Flávio Faria De Moraes

Authors
  1. Gisella Maria Zanin
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  2. Flávio Faria De Moraes
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Zanin, G.M., De Moraes, F.F. Modeling cassava starch saccharification with amyloglucosidase. Appl Biochem Biotechnol 57, 617–625 (1996). https://doi.org/10.1007/BF02941742

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