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Conversion of lignocellulosics pretreated with liquid hot water to ethanol

  • Session 1 Thermal, Chemical, and Biological Processing
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Abstract

Lignocellulosic materials pretreated using liquid hot water (LHW) (220°C, 5 MPa, 120 s) were fermented to ethanol by batch simultaneous saccharification and fermentation (SSF) usingSaccharomyces cerevisiae in the presence ofTrichoderma reesei cellulase. SSF of sugarcane bagasse (as received), aspen chips (smallest dimension 3 mm), and mixed hardwood flour (−60 +70 mesh) resulted in 90% conversion to ethanol in 2–5 d at enzyme loadings of 15–30 FPU/g. In most cases, 90% of the final conversion was achieved within 75 h of inoculation. Comminution of the pretreated substrates did not affect the conversion to ethanol. The hydrolysate produced from the LHW pretreatment showed slight inhibition of batch growth ofS. cerevisiae. Solids pretreated at a concentration of 100 g/L were as reactive as those pretreated at a lower concentration, provided that the temperature was maintained at 220°C.

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Authors and Affiliations

  1. Thayer School of Engineering, Dartmouth College, 03755, Hanover, NH

    G. Peter van Walsum, Mark S. Laser & Lee R. Lynd

  2. Hawaii Natural Energy Institute, University of Hawaii at Manoa, Honolulu, HI

    Stephen G. Allen, Mark J. Spencer & Michael J. Antal

Authors
  1. G. Peter van Walsum
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  2. Stephen G. Allen
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  3. Mark J. Spencer
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  4. Mark S. Laser
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  5. Michael J. Antal
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  6. Lee R. Lynd
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van Walsum, G.P., Allen, S.G., Spencer, M.J. et al. Conversion of lignocellulosics pretreated with liquid hot water to ethanol. Appl Biochem Biotechnol 57, 157–170 (1996). https://doi.org/10.1007/BF02941696

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