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The effect of external reflux on the foam fractionation of proteins

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Abstract

Foam fractionation of proteins is especially advantageous in treating dilute solutions where other separation methods run into economical limitations. Improvement in separation efficiency can be obtained by adding external reflux to the column. This work studied operational variables, such as superficial gas velocity and solution concentration, on the enrichment of the foam phase when external reflux was added to the separation. Bovine serum albumin, cytochrome C, and barley malt α-amylase were the proteins fractionated in a column 3.2 cm in diameter and 105 cm in height operated by bubbling nitrogen in pH-controlled solutions. The determination of solute enrichment and purification factor for several external reflux ratios demonstrated the importance of this operational variable on the efficiency of foam fractionation of proteins.

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Authors and Affiliations

  1. Departamento de Termofluidodinâmica, Universidade Estadual de Campinas Caixa, Postal 6066, CEP 13081-970, Campinas, SP, Brazil

    Sérgio Luiz de Lucena & Cesar Costapinto Santana

  2. Departamento de Processos Químicos, Faculdade de Engenharia Química, Universidade Estadual de Campinas Caixa, Postal 6066, CEP 13081-970, Campinas, SP, Brazil

    Everson Alves Miranda

Authors
  1. Sérgio Luiz de Lucena
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  2. Everson Alves Miranda
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  3. Cesar Costapinto Santana
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de Lucena, S.L., Miranda, E.A. & Santana, C.C. The effect of external reflux on the foam fractionation of proteins. Appl Biochem Biotechnol 57, 57–65 (1996). https://doi.org/10.1007/BF02941688

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  • DOI: https://doi.org/10.1007/BF02941688

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