Abstract
The glucose consumption in tumoursin vivo as reflected by uptake of [18F]2-fluoro-2-deoxy-D-glucose (18FDG) using positron emission tomography (PET) is currently under investigation as a measure of tumour response to radiotherapy. The calculation of cerebral metabolic rate of glucose from18FDG-PET data requires a proportionality factor referred to as the lumped constant. In the presentin vitro study, the utilizations of18FDG and glucose have been measured in a human glioblastoma cell line (86HG-39) as a function of γ-radiation dose with various post-irradiation times and of different fractionation modes. The ratio of utilization of18FDG to that of glucose (RF/G), assumed to correspond to the lumped constant, was observed to increase 12 and 24 h after single fraction γ-exposure by factors ranging from 1.2 to 1.5 compared with the non-irradiated controls. It decreased after multiple fraction γ-exposure (4 × 2 Gy) by a factor of 0.7 compared with the single fraction schedule (1 × 8 Gy). The results suggest that the affinities of glucose transporters or hexokiriase enzyme or both for18FDG and glucose could be influenced by γ-irradiation in this tumour cell linein vitro. Apparent changes of the glucose consumption determined with PET in human tumours following radiotherapy may, therefore, not be solely due to changes in cellular metabolism or cell number but may also be due to changes in RF/G.
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Gjedde A, Wienhard K, Heiss W-D, Kloster G, Diemer N H, Herholz K and Pawlik G 1985 Comparative regional analysis of 2-fluorodeoxyglucose and methylglucose uptake in brain of four stroke patients with special references to the regional estimation of the lumped constant;J. Cereb. Blood Flow Metabol. 5 163–178
Hamacher K, Coenen H H and Stöcklin G 1986 Efficient stereospecific synthesis of no-carrier-added 2-[18F]fluoro-2-deoxy-D-glucose using aminopolyether supported nucleophilic substitution;J. Nucl. Med. 27 235–238
Herholz K, Wienhard K and Heiss W-D 1990 Validity of PET studies in brain tumours;Cerebrovasc. Brain Metabol. Rev. 2 240–265
Kapoor R, Spence A M, Muzi M, Graham M M, Abbott G L and Krohn K A 1989 Determination of the deoxyglucose and glucose phosphorylation ratio and the lumped constant in rat brain and a transplantable rat glioma;J. Neurochem. 53 37–44
Langen K-J, Roosen N, Kuwert T, Herzog H, Kiwit J C W, Rota Kops E, Muzik O, Bock W J and Feinendegen L E 1989 Early effects of intra-arterial chemotherapy in patients with brain tumours studied with pet: preliminary results;Nucl. Med Commun. 10 779–790
Lucignani G, Schmidt K C and Moresco R M 1993 Measurement of regional cerebral glucose utilization with [18F]FDG and PET in heterogeneous tissue: Theoretical considerations and practical procedures;J. Nucl. Med. 34 360–369
Nalimov V V 1972 Ausreissertest nach Nalimov; inElementare Tests zur Beurteilung von Messdaten (eds) RKaiser and GGottschalk (Wein: Bibliographisches Institut Mannheim) pp 18–21
Reivich M, Alavi A, Wolf A, Fowler J, Russell J, Arnett C, MacGregor R R, Shiue C Y, Atkins A, Anand A, Dann R and Greenberg J H 1985 Glucose metabolic rate kinetic model parameter determination in humans: the lumped constants and rate constants for [18F]Fluorodeoxyglucose and [11C]Deoxyglucose;J. Cereb. Blood Flow Metabol. 5 179–192
Schmidt K C, Lucignani G and Sokoloff L 1996 Fluorine-18-Fluocodeoxyglucose PET to determine regional cerebral glucose utilization: A re-examination;J. Nucl. Med. 37 394–399
Sokoloff L, Reivich M, Kennedy C, Rosier M H D, Patlak C S, Pettigrew K D, Sakurada O and Shinohara M 1977 The [14C]deoxyglucose method for the measurement of local cerebral glucose utilization: Theory, procedure, and normal values in the conscious and anesthetized albino rat;J. Neurochem. 28 897–916
Som P, Atkins H L and Bandoypadhyay D 1980 A fluorinated glucose analog, 2-[18F]Fluoro-2-deoxy-D-glucose: nontoxic tracer for rapid tumor detection;J. Nucl. Med. 21 670–675
Spence A M, Muzi M, Graham M M, Freeman S D, Krohn K A, Link J M, Lewellen T K and OíSullivan F 1995 MRGIc and FDG lumped constant in malignant gliomas. Response to radiotherapy;J. Cereb. Blood Flow Metabol. 15 S174
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Schneeweiss, F.H.A., Sharan, R.N., Hautzel, H. et al. Effect of γ-radiation on the ratio of [18F]2-fluoro-2-deoxy-D-glucose to glucose utilization in human glioblastoma cellsin vitro . J. Biosci. 24, 49–52 (1999). https://doi.org/10.1007/BF02941106
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DOI: https://doi.org/10.1007/BF02941106