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Factor IV—ionised calcium—its requirement for glass-contacting of plasma

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Summary

AFTER prolonged dialysis of citrated platelet-free non-contact plasma against isotonic saline, its behaviour on recalcification alone and in the presence of added platelets, tissue thromboplastin, and long-chain fatty acids was relatively unchanged, but in the presence of a glass surface its behaviour was quite different. Unlike native plasma or citrated plasma, dialysed plasma was insensitive and could be incubated in glass apparatus with little or no contact activation, the amount of change induced being inversely proportional to the duration of the dialysing process. When such dialysed plasma was exposed to glass in the presence of ionised calcium at suitable low concentration, contacting occurred and coagulation on subsequent full recalcification in siliconed apparatus was much accelerated. With calcium citrate the contact activity induced was proportional to the concentration of calcium during the glass-contact stage. The total activity was related to the ionic strength of the electrolytes in the plasma test mixture. The system was sensitive to the monovalent cation species present, sodium being appreciably more active than potassium in promoting coagulation.

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  1. Department of Experimental Medicine, Trinity College, Saint Luke’ s Hospital, 6, Rathgar, Dublin

    W. A. Boggust & R. A. Q. O’Meara

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  1. W. A. Boggust
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  2. R. A. Q. O’Meara
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Boggust, W.A., O’Meara, R.A.Q. Factor IV—ionised calcium—its requirement for glass-contacting of plasma. Ir J Med Sci 140, 491–504 (1971). https://doi.org/10.1007/BF02939857

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