High amount of dietary carbohydrate does not cause an adaptational increase of stimulated human pancreatic amylase secretion

  • Ralf-Marco Liehr
  • Maria-Elisabeth Herrmann
  • Carsten Emde
  • Rüdiger Hopert
  • Ernst-Otto Riecken


In order to investigate whether the human pancreas is capable of adapting to a diet with high-carbohydrate, low-fat, and normal protein content, 10 healthy volunteers were given a defined elemental diet (60% of calories as carbohydrates, 22% as fat, and 18% as protein) for 7 d. For the next 7 d they received an elemental diet with a further increased carbohydrate content (76% of calories) and a decreased fat content (10% of calories). A complete secretin-pancreozymin test was carried out at the end of the first wk and at d 14. The results show that an increase in dietary carbohydrate does not provoke an adaptational response of stimulated secretion rates of amylase, trypsin, and chymotrypsin in humans, as expected from animal experiments.

Key Words

Amylase secretion adaptation elemental diet diet composition 


  1. 1.
    Sommer H, Kasper H. Dietary effects on pancreatic exocrine function. Ann. Nutr. Metab. 1981; 25: 381–388.PubMedCrossRefGoogle Scholar
  2. 2.
    Vidon M, Hecketsweiler P, Butel J, Bernier JJ. Effect of continuous jejunal perfusion of elemental and complex nutritional solutions on pancreatic enzyme secretion in human subjects. Gut 1978; 19: 194–198.CrossRefGoogle Scholar
  3. 3.
    Perrault J, Devroede G, Bounous G. Effects of an elemental diet in healthy volunteers. Gastroenterology 1973; 64: 569–576.PubMedGoogle Scholar
  4. 4.
    Emde C, Liehr R-M, Gregor M, Pleul O, Riecken EO, Menge H. Lack of adaptive changes in human pancreatic amylase and lipase secretion in response to high-carbohydrate, low-fat diet applied by a 10-day continuous intraduodenal infusion. Dig. Dis. Sci. 1985; 30: 204–210.PubMedCrossRefGoogle Scholar
  5. 5.
    Bernfeld P. Amylases. Colowick CD, Caplan ND, eds., Methods in enzymology. Academic Press, NY, 1955: 149–158.CrossRefGoogle Scholar
  6. 6.
    Hummel BCW. A modified spectrophotometric determination of chymotrypsin, trypsin, and thrombin. Can. J. Biochem. Physiol. 1959; 37: 1393–1399.PubMedGoogle Scholar
  7. 7.
    Soergel KH, Hogan WJ. On the suitability of poorly absorbed markers as dilution indicators in the gastrointestinal tract. Gastroenterol. 1967; 52: 1056–1057.Google Scholar
  8. 8.
    Hyden S. A turbidometric method for determination of higher polyethylene glycols in biological materials. Ann. R. Agric. Coll. Sweden 1955; 22: 139–145.Google Scholar
  9. 9.
    Otte M, Stahlheber H, Zoelch M, Forell MM. Pankreassekretion des Menschen nach Stimulation mit Cholecystokinin/Pankreozymin, Sekretin und Galle. Methodik und Normalwerte, Klin. Wschr. 1973; 51: 915–920.CrossRefGoogle Scholar
  10. 10.
    Bortz J. Lehrbuch der empirischen Forschung. Springer, Berlin, 1984.Google Scholar
  11. 11.
    Sachs L. Angewandte Statistik. Springer, Berlin, 1974.Google Scholar
  12. 12.
    Grossman MI, Greengard H, Iry AC. The effect of dietary composition on pancreatic enzymes. Am. J. Physiol. 1943; 138: 676–682.Google Scholar
  13. 13.
    Christophe J, Camus J, Deschodt-Lanckman M, Rathe J, Robberecht P, Vandermeers-Piret MC, Vandermeers A. Factors regulating biosynthesis, intracellular transport, and secretion of amylase and lipase in the rat exocrine pancreas. Horm. Metab. Res. 1971; 3: 393–403.PubMedCrossRefGoogle Scholar
  14. 14.
    St<öckmann F, and S<öling HD. Regulation of biosynthesis of trypsinogen and chymotrypsinogen by nutritional and hormonal factors in the rat. Eur. J. Clin, Invest. 1981; 11: 121–132.CrossRefGoogle Scholar
  15. 15.
    Bourdel G. Effects of separate feeding of proteins and lipids on pancreatic adaptation in the rat. Am. J. Physiol. 1983; 244: G125-G130.PubMedGoogle Scholar
  16. 16.
    Poort SR, Poort C. Effect of diet composition on the protein synthetic pattern of the rat pancreas after a feeding period of five days. Biochim. Biophys. Acta 1980; 606: 138–147.Google Scholar
  17. 17.
    Snook JT. Dietary regulation of pancreatic enzymes in the rat with emphasis on carbohydrate. Am. J. Physiol. 1971; 221: 1383–1387.PubMedGoogle Scholar
  18. 18.
    Haberich FJ, Bozkurt T. Physiological studies of the exocrine pancreatic secretion in conscious rats. 6. Communication: Steady-state adaptation to different food composition. Z. Gastroenterol. 1984; 22: 259–267.PubMedGoogle Scholar
  19. 19.
    Saraux B, Girard-Globa A, Ouagued M, Vacher D. Response of the exocrine pancreas to quantitative and qualitative variations in dietary lipids. Am. J. Physiol. 1982; 243: G10-G15.PubMedGoogle Scholar
  20. 20.
    Allan BJ, White TT. A demonstration that the ratios between human pancreatic enzymes can change in 4 days. Digestion 1976; 14: 232–241.PubMedCrossRefGoogle Scholar
  21. 21.
    Lahaie RG, Dagorn JC. Dietary regulation of pancreatic protein synthesis. II. Kinetics of adaptation of protein synthesis and its effect on enzyme content. Biochim. Biophys. Acta 1981; 654: 119–213.PubMedGoogle Scholar
  22. 22.
    Vidon N, Pfeiffer A, Franchisseur C, Bovet M, Rongier M, Bernier JJ. Effect of different caloric loads in human jejunum on meal-stimulated and nonstimulated biliopancreatic secretion. Am. J. Clin. Nutr. 1988; 47: 400–405.PubMedGoogle Scholar
  23. 23.
    Tuten MB, Wogt ST, Dasse F, Leider Z. Utilization of prealbumin as a nutritional parameter. JPEN 1985; 9: 709–711.Google Scholar
  24. 24.
    Bach AC, Babayan VK, Medium-chain triglycerides: an update. Am. J. Clin. Nutr. 1982; 36: 950–962.PubMedGoogle Scholar
  25. 25.
    Winitz M, Seedeman DA, Graff J. Studies in metabolic nutrition employing chemically defined diet. Extended feeding of normal human adult males. Am. J. Clin. Nutr. 1970; 23: 525–545.PubMedGoogle Scholar

Copyright information

© Humana Press Inc. 1989

Authors and Affiliations

  • Ralf-Marco Liehr
    • 1
  • Maria-Elisabeth Herrmann
    • 1
  • Carsten Emde
    • 1
  • Rüdiger Hopert
    • 1
  • Ernst-Otto Riecken
    • 1
  1. 1.Medizinische Klinik und PoliklinikKlinikum Steglitz der FU BerlinBerlin 45FRG

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