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A rapid, convenient enzyme linked immunosorbent assay for total immunoglobulin a in serum

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Summary

A Specific, sensitive and convenient Elisa for total IgA in serum, carried out on microtitre plates and based on commercially available reagents, was developed and extensively validated. The cross-reactivity of both IgG and IgM was much less than 0.1% and other serum proteins were undetectable. The detection limit was 0.18 ng/well or 90 micrograms/ml of serum, allowing for pre-dilution. Sera from normal (1.4-4.1 mg/ml), coeliacs (1.6-13.52 mg/ml) and IgA deficient individuals (0.05-0.4 mg/ ml) were analysed.

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References

  • Ammann, A. J. and Hong, R. 1971. Selective IgA deficiency-Presentation of 30 cases and a review of the literature. Medicine, 50, 223.

    Article  PubMed  CAS  Google Scholar 

  • Asquith, P., Thompson, R. A. and Cooke, W. T. 1969. Serum immunoglobulins in adult coeliac disease. Lancet ii, 129.

    Article  Google Scholar 

  • Baklien, K., Brandtzaeg, P. and Fausa, G. 1977. Immunoglobulins in jejunal mucosa and serum from patients with adult coeliac disease. Scand. J. Gastro. 12, 149–159.

    CAS  Google Scholar 

  • Cleere, W. F., Gosling, J. P., Morris, M. C., Charleton, M. F., Moloney, B. T., Fottrell, P. F. and Sreenan, J. M. 1985.A high performance, highfi throughput enzyme immunoassay for the analysis of progesterone in pasma or milk. Ir. Vet. J. 39, 6–14.

    Google Scholar 

  • Delacroix, D. L. and Vaerman, J. J. 1982. Influence of molecular size of IgA on its immunoassay by various techniques. III. Immunonephelometry. J. Immunol. Methods 51, 49–55.

    Article  CAS  Google Scholar 

  • Delacroix, D. L., Dehennin, J. P. and Vaerman, J. P. 1982. Influence of molecular size of IgA on its immunoassay by various techniques. II. Solid-phase radioimmunoassay. J. Immunol. Methods 48, 327–337.

    CAS  Google Scholar 

  • Eildelman, S., Davis, S. D., Lagunoff, D. and Rubin, C. E. 1966. The relationship between intestinal plasma cells and serum immunoglobulin A (IgA) in man. J. Clin. Invest. 45, 1003–1004.

    Google Scholar 

  • Hobbs, J. R. 1984. News Sheet, Association of Clinical Biochemists. 252, 17.

    Google Scholar 

  • Ishiguro, Y., Kanefusa, K. and Takahiro, I. 1981. Sensitive solid phase enzyme immunoassay for human IgA, secretory IgA and secretory component. Clin. Chim. Acta 116, 237–243.

    Article  PubMed  CAS  Google Scholar 

  • Kricka, J. L., Carter, T. N. J., Burt, S. M., Kennedy, J. H., Holder, R. L., Halliday, M. I., Telford, M. E. and Wisdom, G. B. 1980. Variability in the adsorbtion properties of microtitre plates used as solid supports in enzyme immunoassay. Clin. Chem. 26, 741–744.

    PubMed  CAS  Google Scholar 

  • Laurell, C. B. 1966. Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Anal. Biochem. 15, 45–52.

    Article  PubMed  CAS  Google Scholar 

  • Lems-Van Kan, P., Verspaget, H. W. and Pena, A. S. 1983. ELISA for quantitative measurement of human immunoglobulins IgA, IgG, and IgM in nanograms. J. Immunol. Methods 57, 51–57.

    Article  PubMed  CAS  Google Scholar 

  • Mancini, G., Carlionara, A. O. and Heremans, J. F. 1965. Immuno chemical quantitation of antigens by single radial immunodiffusion. Immunochemistry 2, 235–254.

    Article  PubMed  CAS  Google Scholar 

  • Power, M. J., Cleere, W. F., Gosling, J. P., Fottrell, P. F., Langley, O. H. and Sreenan, J. M. 1985. A direct high throughput, enzyme immunoassay for oestrone sulphate in the milk of cows. Ir. Vet. J. 39, 18–24.

    Google Scholar 

  • Roitt, J. M. 1980. Essential Immunology. London, Blackwell Scientific Publications. p. 39.

    Google Scholar 

  • Sack, D. A., Neogli, P. K. B. and Khorshed Alam, M. D. 1880. Immunobead enzyme linked immunosorbent assay for quantitating immunoglobulin A in human secretions and serum. Infect. Immun. 29, 281, 283.

    Google Scholar 

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Authors and Affiliations

  1. Department of Medicine, University College, Galway

    M. C. Booth, J. P. Gosling, P. F. F. Fottrell & C. F. McCarthy

  2. Department of Biochemistry, University College, Galway, Ireland

    M. C. Booth, J. P. Gosling, P. F. F. Fottrell & C. F. McCarthy

Authors
  1. M. C. Booth
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  2. J. P. Gosling
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  3. P. F. F. Fottrell
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  4. C. F. McCarthy
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Additional information

We wish to thank the Medical Research Council of Ireland for supporting this project as part of its funding of the Centre for Digestive Disorders, Galway, and for a postgraduate funding grant to M.C.B. We also wish to thank Dr. Fiona Stevens and Dr. Eilis Cryan. J.P.G. acknowledges the Agricultural Institute for a grant in aid.

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Booth, M.C., Gosling, J.P., Fottrell, P.F.F. et al. A rapid, convenient enzyme linked immunosorbent assay for total immunoglobulin a in serum. I.J.M.S. 154, 146–152 (1985). https://doi.org/10.1007/BF02937257

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  • DOI: https://doi.org/10.1007/BF02937257

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