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Reduced gastric surface mucus layer in experimental portal hypertension

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Abstract

The pathogenesis of portal hypertensive gastropathy has not yet been thoroughly elucidated. Changes in the gastric surface mucus layer in prehepatic portal hypertensive and cirrhotic rat models were studied by observing frozen sections fixed with formaldehyde vapor and stained with hematoxylin and eosin. We produced prehepatic portal hypertensive rats by partial ligation of the portal vein, and cirrhotic rats by prolonged administration of carbon tetrachloride (CCl4) and phenobarbital sodium. The thickness of the corporal and antral gastric surface mucus was significantly reduced in prehepatic portal hypertensive and cirrhotic rats compared with the values obtained in control rats for portal hypertension (subjected to sham operation) and control rats for cirrhosis (treated with phenobarbital but not CCl4). These results indicate that the hemodynamic changes associated with portal hypertension reduce the thickness of the gastric surface mucus layer and may be one of the causes of the gastropathy associated with portal hypertension.

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References

  1. McCormack TT, Sims J, Eyre-Brook I, et al. Gastric lesions in portal hypertension: Inflammatory or congestive? Gut 1985;26: 1226–1232.

    Article  PubMed  CAS  Google Scholar 

  2. Sato I, Imamura M, Sasaki I, et al. Gastric acid secretion and gastric inhibitory polypeptide release in cirrhotic patients. Am J Gastroenterol 1985;80:163–169.

    PubMed  CAS  Google Scholar 

  3. Gaur SK, Vij JC, Sarin SK, et al. Gastric secretion in cirrhosis and non-cirrhotic portal fibrosis. Digestion 1988;39:151–155.

    Article  PubMed  CAS  Google Scholar 

  4. Blanco CDV, Caporaso N, Mele A, et al. Morphologic and functional damage of gastric mucosa in liver cirrhosis. Panminerva Med 1983;25:225–230.

    Google Scholar 

  5. Arakawa T, Satoh H, Fukuda T, et al. Endogenous prostaglandin E2 in gastric mucosa of patients with alcoholic cirrhosis and portal hypertension. Gastroenterology 1987;93:135–140.

    PubMed  CAS  Google Scholar 

  6. Levine BA, Schwesinger WH, Sirinek KR. Hemorrhagic gastritis in cirrhotics: A prostaglandin connection? Surg Res Commun 1991;10:15–20.

    Google Scholar 

  7. Sarfeh IJ, Malki A, Tarnawski A, et al. Portal hypertension and gastric mucosal injury in rats: Effects of alcohol. Gastroenterology 1983;84:987–993.

    PubMed  CAS  Google Scholar 

  8. Sarfeh IJ, Tarnawski A, Hadjuczek A, et al. The portal hypertensive gastric mucosa: Histologic, ultrastructural and functional analysis after aspirin-induced damage. Surgery 1988;104:79–85.

    PubMed  CAS  Google Scholar 

  9. Sarfeh IJ, Tarnawski A, Maeda R, et al. The gastric mucosa in portal hypertension: Effects of topical bile acid. Scand J Gastroenterol 1984;19(Suppl 92): 189–194.

    Google Scholar 

  10. Kerss S, Allen A, Garner A. A simple method for measuring thickness of the mucus gel layer adherent to rat, frog and human gastric mucosa: Influence of feeding, prostaglandin, N-acetylcysteine and other agents. Clin Sci 1982;63:187–195.

    PubMed  CAS  Google Scholar 

  11. Flemstrom G, Garner A. Gastroduodenal HCO3 transport: Characteristics and proposed role in acidity regulation and mucosal protection. Am J Physiol 1982;242:G183-G193.

    PubMed  CAS  Google Scholar 

  12. Allen A, Hutton DA, Leonard AJ, et al. The role of mucus in the protection of the gastroduodenal mucosa. Scand J Gastroenterol 1986;(Suppl 125): 71–77.

    Article  Google Scholar 

  13. Allen A. Gastrointestinal mucus. In: Rauner BB (ed). Handbook of physiology. The gastrointestinal system, vol. 3, Salivary, gastric, pancreatic and hepatobiliary secretion. New York: Oxford University Press, 1989;359–382.

    Google Scholar 

  14. Sakata T, Engelhardt WV. Luminal mucin in the large intestine of mice, rats and guinea pigs. Cell Tissue Res 1981;219:629–635.

    PubMed  CAS  Google Scholar 

  15. Ota H, Katsuyama T. Alternating laminated array of two types of mucin in the human gastric mucous layer. Histochem J 1992;24:86–92.

    Article  PubMed  CAS  Google Scholar 

  16. Chojkier M, Groszmann RJ. Measurement of portal systemic shunting in the rat by using g-labeled microspheres. Am J Physiol 1981;240:G371-G375.

    PubMed  CAS  Google Scholar 

  17. McLean EK, McLean AEM, Sutton PM. Instant cirrhosis. An improved method for producing cirrhosis of the liver in rats by simultaneous administration of carbon tetrachloride and phenobarbitone. Br J Exp Pathol 1969;50:502–506.

    PubMed  CAS  Google Scholar 

  18. Ma JJ, Cho CH, Ogle CW. Portal hypertension: Its effects on gastric function and ethanol-induced gastric mucosal damage in rats. Dig Dis Sci 1993;38:2203–2208.

    Article  PubMed  CAS  Google Scholar 

  19. Allen A. The structure and function of gastrointestinal mucus. In: Harmon JW (ed) Basic mechanisms of gastrointestinal mucosal cell injury and protection. Baltimore: William and Wilkins, 1981;351–367.

    Google Scholar 

  20. McQueen S, Hutton D, Allen A, et al. Gastric and duodenal surface mucus gel thickness in rat: Effects of prostaglandins and damaging agents. Am J Physiol 1983;245:G388-G393.

    PubMed  CAS  Google Scholar 

  21. Morris GP, Wallace JL. The roles of ethanol and of acid in the production of gastric mucosal erosions in rats. Virchows Arch B Cell Pathol 1981;38:23–38.

    Article  CAS  Google Scholar 

  22. Lacy ER, Ito S. Microscopic analysis of ethanol damage to rat gastric mucosa after treatment with prostaglandin. Gastroenterology 1982;83:169–625.

    Google Scholar 

  23. Florey H. Mucin and the protection of the body. Proc R Soc Lond [Biol] 1955;143:619–625.

    Article  Google Scholar 

  24. Bickel M, Kaufman GL. Gastric gel mucus thickness: Effect of distention, 16,16-dimethyl prostaglandin E2, and carbenoxolone. Gastroenterology 1981;80:770–775.

    PubMed  CAS  Google Scholar 

  25. Sellers LA, Carroll NJH, Allen A. Misoprostol-induced increases in adherent gastric mucus thickness and luminal mucus output. Dig Dis Sci 1986;31:91S-95S.

    Article  PubMed  CAS  Google Scholar 

  26. Allen A, Ward R, Cunliffe WJ, et al. Changes in adherent mucus gel and pepsinolysis in peptic ulcer patients (abstract). Dig Dis Sci 1985;30:365.

    Article  Google Scholar 

  27. Younan F, Pearson J, Allen A, et al. Changes in the structure of the mucus gel on the mucosal surface of the stomach in association with peptic ulcer disease. Gastroenterology 1982;82:827–831.

    PubMed  CAS  Google Scholar 

  28. Sandzén B, Blom H, Dahlgren S. Gastric mucus gel layer thickness measured by direct light microscopy. An experimental study in the rat. Scand J Gastroenterol 1988;23:1160–1164.

    Article  PubMed  Google Scholar 

  29. Sikuler E, Kravetz D, Grossmann RJ. Evolution of portal hypertension and mechanisms involved in its maintenance in a rat model. Am J Physiol 1985;248:G618-G625.

    PubMed  CAS  Google Scholar 

  30. Kameyama J, Suzuki Y, Suzuki A, et al. Gastric mucus secretion in portal hypertension. J Gastroenterol Hepatol 1989;Suppl 1: 126–128.

    Google Scholar 

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Authors and Affiliations

  1. Second Department of Surgery, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, 113, Tokyo, Japan

    Hiroaki Imanishi, Yasushi Harihara, Yasutsugu Bandai, Kensho Sanjo & Masatoshi Makuuchi

Authors
  1. Hiroaki Imanishi
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  2. Yasushi Harihara
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  3. Yasutsugu Bandai
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  4. Kensho Sanjo
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  5. Masatoshi Makuuchi
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Additional information

This article is based on a study first reported in abbreviated form. Imanishi H, Sanjo K. The gastric mucus in portal hypertension. In: Aoki H, Kobayashi M (eds) The pathophysiology and management of esophageal and gastric varices (in Japanese). Tokyo: Igakushoin, 1996; 120–122.

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Imanishi, H., Harihara, Y., Bandai, Y. et al. Reduced gastric surface mucus layer in experimental portal hypertension. J Gastroenterol 32, 720–725 (1997). https://doi.org/10.1007/BF02936946

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  • DOI: https://doi.org/10.1007/BF02936946

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