Pilot-scale studies of sugar separations by continuous chromatography

  • Charles H. Byers
  • Warren G. Sisson
  • Joseph P. Decarli
  • Giorgio Carta
Session 3 Bioengineering research


A pilot-scale continuous Chromatograph has been developed for optimization studies and scaling factor evaluations. The continuous annular Chromatograph consists of a slowly rotating annular bed of ion exchange resin to which feed is continuously introduced at a stationary point at the top of the bed while eluent flows over the remainder of the annulus. The rotation of the sorbent bed coupled with the simultaneous elution chromatography causes the separated components of the feed stream to appear as helical bands, each of which has a characteristic, stationary exit point.

The separation of sucrose, glucose, and fructose, using the calcium form of cationic ion exchange resin, uses water as the isocratic eluent. A synthetic mixture of the sugar was explored in fixed columns, then on a bench scale unit. The results almost perfectly scaled to the pilot unit when loading was low and feed mixtures were of low viscosity. Factors such as column loading, feed-to-eluent ratio, and feed concentration were explored. An industrial sugar mixture containing the three sugars and a number of higher molecular sugars was successfully separated. Based on these results, recommendations are made concerning an optimized scaling of the process.

Index Entries

Chromatography ion exchange sugars separations scaleup 



Particle-fluid interfacial area, cm2


Liquid-phase solute concentration, g/cm3


Feed solute concentration, g/cm3


Equilibrium concentration, g/cm3


Axial dispersion coefficient, g/cm2/s

Angular dispersion coefficient, g/cm2


Global mass transfer coefficient, cm/s


Equilibrium distribution coefficient


Sorbent solute concentration, gsolute/gsolid


Column loading of solute, gsolute/gsolid


Feed flowrate, g/s


Total flowrate, g/s


Outside radius of annular bed, cm


Time, s

\(\hat t\)

Chromatographie time, s


Superficial velocity, cm/s


Bed axial position, cm

Bed void fraction


Displacement from feed point, radians


Elution angle, radians


Feed angle, radians


Rotation rate, radians/s


  1. 1.
    Begovich, J. M. (1982), Ph.D. dissertation, The University of Tennessee, Knoxville, TN.Google Scholar
  2. 2.
    Begovich, J. M., Byers, C. H., and Sisson, W. G. (1983),Sep. Sci. Technol. 18, 1167.CrossRefGoogle Scholar
  3. 3.
    Broughton, D. B. (1966), US patent 3,291,726.13.Google Scholar
  4. 4.
    Carta, G. and Byers, C. H. (1988), “Novel Applications of Continuous Annular Chromatography,” to be published, ACS Symposium Series,New Directions in Sorption Technology, ed. G. E. Keller II and R. T. Yang.Google Scholar
  5. 5.
    Ching, C. B. and Ruthven, D. M. (1984),Can. J. Chem. Eng. 62, 398.CrossRefGoogle Scholar
  6. 6.
    Ching, C. B., Ruthven, D. M., and Hidajat, K. (1985),Chem. Eng. Sci. 40, 1411.CrossRefGoogle Scholar
  7. 7.
    Hashimoto, K., Adachi, S., Noujima, H., and Marayuma, H. (1983),J. Chem. Eng. Japan 16, 400.CrossRefGoogle Scholar
  8. 8.
    Heikkila, H. (1983),Chem. Eng. 24, 50.Google Scholar
  9. 9.
    Howard, A. J. (1987), MS Thesis, University of Virginia, Charlottesville, VA.Google Scholar
  10. 10.
    Howard, A. J., Carta, G., and Byers, C. H. (1987), “Separation of Sugars by Continuous Annular Chromatography,” Oak Ridge National Laboratory Report ORNL/TM-10318.Google Scholar
  11. 11.
    Lehninger, A. L. (1977),Biochemistry, Worth, New York.Google Scholar
  12. 12.
    Ruthven, D. M. (1984),Principles of Adsorption and Adsorption Processes, Wiley, New York.Google Scholar
  13. 13.
    Rohm and Haas Co. (1979),Amberlite Ion Exchange Resins Laboratory Guide, Philadelphia, PA.Google Scholar
  14. 14.
    Sherwood, R. K., Pigford, R. L., and Wilke, C. R. (1975), MassTransfer, McGraw-Hill, New York.Google Scholar
  15. 15.
    Wankat, P. C. (1977),AIChE J. 23, 859.CrossRefGoogle Scholar
  16. 16.
    Welstein, H. and Sauer, C. (1984),Ion Exchange Technology, ed. Naden, D., and Streat, M., Ellis Harwood, London.Google Scholar

Copyright information

© Humana Press Inc. 1989

Authors and Affiliations

  • Charles H. Byers
    • 1
  • Warren G. Sisson
    • 1
  • Joseph P. Decarli
    • 2
  • Giorgio Carta
    • 2
  1. 1.Chemical Technology DivisionOak Ridge National LaboratoryOak Ridge
  2. 2.Department of Chemical EngineeringUniversity of VirginiaCharlottesville

Personalised recommendations