Abstract
One of the next challenges in the use of biocatalysts (enzyme or microbial cells) is the upgrading of biological reactions of oxidoreduction. The oxidoreductases need cofactors that must be regenerated. Practical experience shows that this is most readily achieved by using living cells of microorganisms.
Living cells ofSaccharomyces cerevisiae are able to bioconvert vanillin to vanillyl alcohol (1). By working with a two-phase reactor (dodecanol—feeding medium) it has been possible to use higher vanillin concentrations without inhibiting the bioconversion (2).
Several parameters, such as, volume ratio of aqueous over organic phase, pH, vanillin concentration seem to influence the bioconversion greatly. Bearing this is mind, two-phase reactors have been set up. Productivities exceeded 5000 g/m3/d.
On the other hand,Saccharomyces cerevisiae mutants have been selected as vanillyl alcohol hyperproducers: vanillyl alcohol productivity of the best selected mutant is twice as high as wild-type strain productivity. Their specific behavior has been studied.
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De Wulf, O., Thonart, P. Bioconversion of vanillin to vanillyl alcohol in a two-phase reactor. Appl Biochem Biotechnol 20, 165–180 (1989). https://doi.org/10.1007/BF02936481
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DOI: https://doi.org/10.1007/BF02936481