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The role of ester groups in resistance of plant cell wall polysaccharides to enzymatic hydrolysis

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Abstract

Xylan backbones in native plant cell walls are extensively acety-lated. Previously, no direct investigations as to their role in cellulolytic enzyme resistance have been done, though indirect results point to their importance. An in vitro deesterification of aspen wood and wheat straw has been completed using hydroxylamine solutions. Yields of 90% acetyl ester removal for both materials have been accomplished, with little disruption of other fractions (i.e., lignin). Apparently, as the xylan becomes increasingly deacetylated, it becomes 5–7 times more digestible. This renders the cellulose fraction more accessible, and 2–3 times more digestible. This effect levels off near an acetyl removal of 75%, where other resistances become limiting.

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References

  1. Aspinall, G. O. (1959),Adv. Carbohydr. Chem. 14, 429.

    CAS  Google Scholar 

  2. Timell, T. E. (1964),Adv. Carbohydr. Chem. 19, 247.

    CAS  Google Scholar 

  3. Timell, T. E. (1965),Adv. Carbohydr. Chem. 20, 409.

    CAS  Google Scholar 

  4. Timell, T. E. (1967),Wood Science Technol. 1, 45.

    Article  CAS  Google Scholar 

  5. Wilkie, K C. B. (1979),Adv. Carbohydr. Chem. 36, 215.

    Article  CAS  Google Scholar 

  6. Lai, Y. Z. and Sarkanen, K. V. (1971),Lignins: Occurrence, Formation, Structure, and Reactions, Sarkanen, K. V. and Ludwig, C. H. eds., Wiley-Inter-science, NY, pp. 165–241.

    Google Scholar 

  7. Erins, P., Cinite, V., Jakobsons, M., and Gravitis, J. (1976),Appl. Polym. Symp. 28, 1117.

    CAS  Google Scholar 

  8. Smith, D. C. C. (1955),Nature. 176, 267.

    Article  CAS  Google Scholar 

  9. Higuchi, T., Ito, Y., Shimada, M., and Kawamura, I. (1967),Phytochem. 6, 1551.

    Article  CAS  Google Scholar 

  10. Higuchi, T., Ito, Y., and Kawamura, I. (1967),Phytochemistry 6, 875.

    Article  CAS  Google Scholar 

  11. Kuwatsuka, S. and Shindo, H. (1973),Soil Sci. Plant Nutr. (Tokyo) 19, 219.

    CAS  Google Scholar 

  12. Whitmore, F. W. (1974),Plant Physiol. 53, 728.

    CAS  Google Scholar 

  13. Markwalder, H. U. and Neukom, H. (1976),Phytochem. 15, 836.

    Article  CAS  Google Scholar 

  14. Harris, P. J. and Hartley, R. D. (1976),Nature 259, 508.

    Article  CAS  Google Scholar 

  15. Theander, O., Uden, P., and Aman, P. (1981),Agric. Environ. 6, 127.

    Article  CAS  Google Scholar 

  16. Hartley, R. D. and Haverkamp, J. (1984),J. Sci. Food Agric. 35, 14.

    Article  CAS  Google Scholar 

  17. Sealbert, A., Monties, B., Rolando, C, and Sierra-Escudero, A. (1986),Holz-forschung 40, 191.

    Google Scholar 

  18. Scalbert, A., Monties, B., Lallemand, J. Y., Guittet, E., and Rolando, C. (1985),Phytochemistry 24, 1359.

    Article  CAS  Google Scholar 

  19. Hartley, R. D. (1972),J. Sci. Food Agric. 23, 1347.

    Article  CAS  Google Scholar 

  20. Hartley, R. D. and Jones, E. C. (1977),Phytochem. 16, 1531.

    Article  CAS  Google Scholar 

  21. Sawai, A., Kondo, T., and Ara, S. (1983),J. Japan Grassl. Sci. 29, 175.

    CAS  Google Scholar 

  22. Morris, E. J. and Bacon, J. S. D. (1977),J. Agric. Sci. Camb.89, 327.

    Google Scholar 

  23. Bacon, J. S. D. and Gordon, A. H. (1980),J. Agric. Sci. Camb.94, 361.

    Article  CAS  Google Scholar 

  24. Bacon, J. S. D., Chesson, A., and Gordon, A. H. (1981),Agric. Environ. 6, 115.

    Article  CAS  Google Scholar 

  25. Chesson, A., Gordon, A. H., and Lomax, J. A. (1983),J. Sci. Food Agric. 34, 1330.

    Article  CAS  Google Scholar 

  26. Agosin, E., Monties, B., and Odier, E. (1985),J. Sci. Food Agric. 36, 925.

    Article  CAS  Google Scholar 

  27. Tarkow, H., Stamm, A. J., and Erickson, E. C. O. Acetylated Wood, US Forest Service Rept. No. 1593, U.S.D.A. Forest Products Laboratory, Publ., Madison, WI (1955), pp. 29.

    Google Scholar 

  28. Stamm, A. J. and Baechler, R. H. (1960),For. Prod. J. 10, 22.

    CAS  Google Scholar 

  29. Goldstein, I. S., Jeroski, E. B., Lund, A. E., Nielson, J. F., and Weaver, J. W. (1961),For. Prod. J. 11, 363.

    CAS  Google Scholar 

  30. Sinner, M., Parameswaran, N., and Dietrichs, H. H. (1979),Hydrolysis of Cellulose: Mechanisms of Enzymatic and Acid Catalysis, Brown, R. D. and Jurasek, L., eds., Adv. in Chemistry No. 181, American Chemical Society Publ., Washington, DC, pp. 303–329.

    Google Scholar 

  31. Biely, P., Puls, J., and Schneider, H. (1985),FEBS Lett. 186, 80.

    Article  CAS  Google Scholar 

  32. Biely, P., MacKenzie, C. R., Puls, J., and Schneider, H. (1986),Bio/Technology 4, 731.

    Article  CAS  Google Scholar 

  33. Biely, P., MacKenzie, C. R., Puls, J., and Schneider, H. (1987),Wood and Cel-lulosics: Industrial Utilization, Biotechnology, Structure, and Properties, Kennedy, J. F., Phillips, G. O., and Williams, P. A., eds., (1987), Horwood, Chichester, UK, pp. 283–289.

    Google Scholar 

  34. Shimizu, K. (1981),J.A.R.Q.; Japan Agricult. Res. Quart. 14, 244.

    CAS  Google Scholar 

  35. Fan, L. T., Lee, Y. H., and Gharpuray, M. M. (1982),Adv. Biochem. Eng.. 23, 157.

    CAS  Google Scholar 

  36. Han, Y. W. (1978),Adv. Appl. Microbiol. 23, 119.

    Article  CAS  Google Scholar 

  37. Millet, M. A., Baker, A. J., and Satter, L. D. (1976),Biotech. Bioeng. Syrnp. 6, 125.

    Google Scholar 

  38. Slavik, I., Pasteka, M., and Kucerova, M. (1967),Faserforsch. Textiltech. 18, 584.

    CAS  Google Scholar 

  39. Siggia, S. and Hanna, J. G. (1979),Quantitative Organic Analysis via Functional Groups, Wiley, New York, pp. 172–183.

    Google Scholar 

  40. Grohmann, K., Himmel, M., Rivard, C, Tucker, M., Baker, J., Torget, R., and Graboski, M. (1985),Biotech. Bioeng. Symp. 14, 137.

    Google Scholar 

  41. Moore, W. E. and Johnson, D. B.Procedures for the Chemical Analysis of Wood and Wood Products, Forest Products Laboratory, USDA, Madison, WI, Report No. 67–045.

  42. Official Test Methods (1983), Technical Association of Pulp and Paper Industry, Atlanta, GA.

  43. Horwitz, W. ed. (1980),Official Methods of Analysis of the Association of Official Analytical Chemists, 13th ed., AOAC, Washington, DC, Method No. 14,064.61

  44. Grohmann, K., Torget, R., and Himmel, M. (1985),Biotech. Bioeng. Symp. 15, 59.

    Google Scholar 

  45. Teese, E. T. and Mandels, M. (1980),Biotechnol. Bioeng. 22, 323.

    Article  Google Scholar 

  46. Wind, R. A., Anthonio, F. E., Duivestijn, M. J., Smidt, J., Trommel, J., and de Vette, G. M. C. (1983),J. Magn. Reson. 52, 424.

    CAS  Google Scholar 

  47. Himmel, M. E. and Oh, K. K. (1983),Biotech. Bioeng. Symp. 13, 583.

    CAS  Google Scholar 

  48. Goldschmid, O. (1971),Lignins: Occurence, Formation, Structure, and Reactions, Sarkanen, K. V. and Ludwig, C. H., eds., Wiley-Interscience, New York, pp. 241–264.

    Google Scholar 

  49. Sterk, H., Sattler, W., and Esterbauer, H. (1987),Carbohydr. Res. 164, 85.

    Article  CAS  Google Scholar 

  50. Haw, J. F., Maciel, G. E., and Schroeder, H. A. (1984),Anal. Chem. 56,1323.

    Article  CAS  Google Scholar 

  51. Ghose, T. K. (1987),Pure and Appl. Chem. 59, No. 2, 257.

    Article  CAS  Google Scholar 

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Grohmann, K., Mitchell, D.J., Himmel, M.E. et al. The role of ester groups in resistance of plant cell wall polysaccharides to enzymatic hydrolysis. Appl Biochem Biotechnol 20, 45–61 (1989). https://doi.org/10.1007/BF02936472

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