Abstract
Recombinant β-glucosidase fromThermus caldophilus GK24 was easily purified partially by a heat treatment procedure, resulting in 8-fold and recovery yield of 80% from crude enzyme. When the β-glucosidase was incubated with a 80% glucose solution (w/w), gentiobiose (β1,6-glucobiose) was the major product in the reaction mixture. The optimal conditions for producing gentiobiose (11% yields of total sugar) were pH 8–9 and 70°C for 72 h.
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Kim, TY., Lee, DS. & Shin, HJ. Gentiobiose synthesis from glucose using recombinant β-glucosidase fromThermus caldophilus GK24. Biotechnol. Bioprocess Eng. 8, 210–212 (2003). https://doi.org/10.1007/BF02935899
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DOI: https://doi.org/10.1007/BF02935899